Name | Poly(D,L-lactide)(PDLLA) |
Synonyms | Poly(D,L-lactide)(PDLLA) |
CAS | 51056-13-9 |
Xu Da-Chuan , Liao Hua , Yan Yuhua , Luo Jiwei
Abstract:
objective: to study the degradation performance of racemic polylactic acid/nerve growth factor slow release catheter (PDLLA/NGF), and to provide the basis for the repair of nerve defects. Methods: PDLLA/NGF slow release catheter was made and used to bridge the sciatic nerve defect in rats. The general observation and electron microscope examination of the catheter were performed at 1,2 and 3 months after operation. Results: postoperative gross observation and electron microscopy showed that PDLLA/NGF slow-release catheter could gradually degrade in rats with time, the inner layer degraded faster than the outer layer, by 3 months, the catheter remained intact, and the regenerated nerve had passed through the catheter lumen without compression and scar formation. Conclusion: the degradation time of PDLLA/NGF catheter in rats is more than 3 months, which can provide the time for nerve regeneration and provide a good microenvironment for nerve regeneration.
Key words:
Poly (l-lactide) nerve growth factor catheter degradation
DOI:
10.3969/j.issn.1001-165X.2006.02.026
cited:
year:
2006
Zhang Jian , Hu Min , Zhang Wenyi
Abstract:
objective to observe the effect of racemic polylactic acid (PDLLA) on the activity of vascular endothelial cells in order to determine whether it can be used as a cell scaffold material for tissue engineering. Methods two-week-old Japanese big-ear white rabbits (provided by the animal experimental center of PLA General Hospital) were collected under aseptic conditions. 1:250 trypsin and 1:100 Type II collagenase 1:1 mixed at 37 °c for 30min, centrifuged (1000r/min) for 10min. The precipitate was added to DMEM medium containing 10% fetal bovine serum to prepare a suspension of vascular endothelial cells, which were placed in a 5% carbon dioxide incubator at 37 ° C. For 72h for liquid change. PDLLA was immersed in 75% alcohol for 4H, distilled water 48H. 1G PDLLA was immersed in DMEM medium containing 0.1% fetal bovine serum for 15ml, and placed in an incubator at 37 ℃,5% carbon dioxide, 7d. Extract the leaching solution, set the leaching solution Group, 1/2 solution group. Aseptically sealed in a 4 ℃ refrigerator for later use. Disinfection good scaffold material was placed in a 24-well petri dish, and vascular endothelial cells were seeded on the scaffold and incubated at 37 °c in a 5% carbon dioxide incubator. The composite cell scaffold material was observed by scanning electron microscope on the 10th day. The third generation of vascular endothelial cells were inoculated into 96-well petri dishes at a concentration of 1 × 10~3, and newly prepared medium was added to the extract solution Group and 1/2 solution Group, respectively. The absorbance and cell viability were measured by 1,3,5, 7D MTT method. Then the relative proliferation rate (RGR) was calculated by the following formula = experimental group absorbance, negative control group absorbance X 100%. Evaluation of toxicity according to RGR value: RGR value ≥ 100% is grade 0, 75%-99% is Grade I, 50%-74% is Grade II, 25%-49% is grade III, 1-24% for grade IV, 0 for Grade V. Results The cells were observed by scanning electron microscope on the 10th day after inoculation, the cells were adsorbed on the surface of the material, the morphology was diverse, there were multiple protrusions, and the cells crossed the surface of the micro-pores or into the pores, the cells are connected by protrusions. The results of cell activity test showed that the scaffold extract had no effect on cell activity, and there was no significant difference in cell activity between the stock solution Group and the extract group (P>0.05).MTT assay confirmed that vascular endothelial cells grew well after 1,3,5, and 7 days of PDLLA extract culture. According to ISO and biological standards of medical materials, the relative proliferation rate of cells indicates that the toxicity of the material is graded as 0 or 1, which indicates that the material has no cytotoxicity and is qualified. Conclusion vascular endothelial cells grow well on the scaffold and can secrete cell matrix. PDLLA has no cytotoxicity and does not affect the growth and differentiation of cells.
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meeting name:
The 4th China International and 7th National Conference on oral and maxillofacial surgery
meeting place:
Chengdu, China
cited: