1,1-diphenyl-2-(2,4,6-trinitrophenyl)diazenium
2,2-Diphenyl-1-picrylhydrazyl
CAS: 1898-66-4
Molecular Formula: C18H12N5O6*
1,1-diphenyl-2-(2,4,6-trinitrophenyl)diazenium - Names and Identifiers
1,1-diphenyl-2-(2,4,6-trinitrophenyl)diazenium - Physico-chemical Properties
| Molecular Formula | C18H12N5O6* |
| Molar Mass | 394.32 |
| Density | 1.484 g/cm3 |
| Melting Point | ~135°C (dec.)(lit.) |
| Water Solubility | Soluble in dimethyl formamide, ethanol, chloroform, ether, carbon disulfinde, acetone, oils, carbon tetrachloride, glacial acetic acid and toluene. Insoluble in water. |
| Solubility | DMSO : 25 mg/mL (63.40 mM; Need ultrasonic) |
| Appearance | Light brown to black (Solid) |
| Color | green |
| Maximum wavelength(λmax) | ['515nm(MeOH)(lit.)'] |
| Merck | 14,3330 |
| BRN | 1846081 |
| Storage Condition | 2-8°C |
| Stability | Shock Sensitive |
| Sensitive | Sensitive to heat and air |
| MDL | MFCD00007231 |
| In vitro study | DPPH shows a strong absorption band at 517 nm due to its odd electron and solution appears a deep violet colour, the absorption vanishes as the electron pairs off. The resulting decolorization is stoichiometric with respect to the number of electrons taken up. The alcoholic solutions of 0.5 mM are densely colored and at this concentration, the Lambert-Beer law is obeyed over the useful range of absorption. DPPH assay is a rapid, simple, inexpensive and widely used method to measure the ability of compounds to act as free radical scavengers or hydrogen donors, and to evaluate antioxidant activity of foods. It can also be used to quantify antioxidants in complex biological systems, for solid or liquid samples. This method is easy and applies to measure the overall antioxidant capacity and the free radical scavenging activity of fruit and vegetable juice. This assay has been successfully utilized for investigating antioxidant properties of wheat grain and bran, vegetables, conjugated linoleic acids, herbs, edible seed oils, and flours in several different solvent systems including ethanol, aqueous acetone, methanol, aqueous alcohol and benzene. It is a convenient method for the antioxidant assay of cysteine, glutathione, ascorbic acid, tocopherol and polyhydroxy aromatic compounds, for olive oil, fruits, juices and wines. |
1,1-diphenyl-2-(2,4,6-trinitrophenyl)diazenium - Risk and Safety
| Hazard Symbols | Xn - Harmful |
| Risk Codes | 42/43 - May cause sensitization by inhalation and skin contact. |
| Safety Description | S22 - Do not breathe dust. S36/37 - Wear suitable protective clothing and gloves. S45 - In case of accident or if you feel unwell, seek medical advice immediately (show the label whenever possible.) |
| UN IDs | UN2811 |
| WGK Germany | 1 |
| RTECS | MW3250000 |
| TSCA | Yes |
| HS Code | 29280000 |
| Hazard Class | 6.1 |
| Packing Group | II |
1,1-diphenyl-2-(2,4,6-trinitrophenyl)diazenium - Reference Information
| EPA chemical information | Information provided by: ofmpub.epa.gov (external link) |
| Application | A,A diphenylB bitter hydrazinyl free radical, namely DPPH radical, is measured at a specific wavelength The change in the absorption value before and after the reaction of DPPH and antioxidant substances can quantitatively determine the antioxidant capacity of the tested substance. DPPH method is a fast and simple method to determine the antioxidant properties of tea. If the determination conditions are properly mastered, better determination stability can be obtained. 2, 2-biphenyl-1-picrylhydrazine is an organic hydrazine compound that can be used as a chemical reagent. |
| Biological activity | DPPH (2,2-Diphenyl-1-picrylhydrazyl) is a stable free radical that can be used to measure the free radical scavenging activity of antioxidants. The odd electrons of the nitrogen atom in DPPH are reduced to the corresponding hydrazine by absorption of the hydrogen atom from the antioxidant. The DPPH method can be used in aqueous and non-polar organic solvents and can be used to examine hydrophilic and lipophilic antioxidants. |
| use | biochemical research |
Last Update：2024-04-10 22:29:15
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View History
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