Solubility | H2O: soluble |
Storage Condition | Room Temprature |
WGK Germany | 3 |
Reference Show more | 1. [IF=6.843] Wenqin Li et al."The antibacterial and antibiofilm activities of mesoporous hollow Fe3O4 nanoparticles in an alternating magnetic field."Biomater Sci-Uk. 2020 Aug;8(16):4492-4507 2. [IF=2.391] Wu Zhihao et al."Preparation of Monascus-fermented ginkgo seeds: optimization of fermentation parameters and evaluation of bioactivity."FOOD SCIENCE AND BIOTECHNOLOGY. 2022 Apr;:1-10 |
appearance | beef maceration powder is a kind of medium raw material, which is made of refined beef and extracted by a new process, and the color is yellow-brown: |
preparation | raw beef powder is prepared from fresh beef by heat treatment, filtration, concentration and drying, the raw beef powder was obtained by hydrolysis, solid-liquid separation, refrigeration, filtration, concentration and drying. |
Application | is mainly used as a medium raw material. 1. An improved formula of blood agar medium, consisting of tryptone, beef extract, liver Digest, yeast extract, starch, sodium chloride, defibrated sheep blood and Agar, the medium contains 10~20g of tryptone, 3~8g of beef extract, 1~4g of liver Digest, 0.5~2g of starch, 3~8g of yeast powder, sodium chloride 3~8G, defibrated sheep blood 40~60ml, Agar 10~20g,pH = 7.2±0.2. In the present invention, the tryptone is made in China, and is prepared from fresh beef and bovine bone by trypsin digestion. It can provide more carbon and nitrogen sources, vitamins and growth factors than peptone; The beef leached powder is made in China, and fresh beef is used as raw material, and the raw beef powder is made by heat treatment, filtration, concentration and drying, the raw beef powder was obtained by hydrolysis, solid-liquid separation, refrigeration, filtration, concentration and drying. Less impurities, can provide organic acids, nucleotides, minerals, vitamins, etc.; The liver digest is imported, liver enzymatic hydrolysate, can provide rich liver glycogen, vitamins and amino acids, it has a significant growth promoting effect on various bacteria, especially for Brucella and amoebic protozoa, and can better culture anaerobic bacteria. The starch is domestic. Starch can provide part of the carbon and nitrogen, and promote the growth of Neisseria and enhance the hemolytic properties of streptococcus, and can absorb some of the toxins produced by bacteria, can make bacteria grow better; the yeast extract powder is imported and fresh yeast is used as raw material, which can provide B vitamins, various amino acids and sugars. The improved formula of the blood agar medium of the present invention can provide comprehensive nutrition, maintain bacterial osmotic pressure, and repair damaged bacteria. The optimized formula of the invention can not only meet the overall needs of common bacteria and most fastidious bacteria, but also facilitate the cultivation and identification of bacteria. 2. A medium for promoting high production of exotoxin by Clostridium perfringens, the medium consisting of the following components in parts by weight: beef extract 18? 30 parts by weight, 6? 15 parts by weight, glucose 4? 8 parts by weight, NaCl 3? 6 parts by weight, water was added to 1000 parts by weight. In a preferred embodiment of the present invention, the pH value of the culture medium is 7.0? 8.5, preferably pH 7.5? 8.0; The Clostridium perfringens is selected from Clostridium perfringens type A, Clostridium perfringens type B, Clostridium perfringens type C, Clostridium perfringens type D, any one of Clostridium perfringens type E or a combination thereof, preferably Clostridium perfringens type C. |