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天门冬氨酸氨基转移酶(AST)检测试剂盒(赖氏微板法)

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天门冬氨酸氨基转移酶(AST)检测试剂盒(赖氏微板法) - Names and Identifiers

天门冬氨酸氨基转移酶(AST)检测试剂盒(赖氏微板法) - Reference

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1. Qiu Xingan, Wang Zhenchang. Experimental study on protective effect of Qushi Hugan prescription on liver injury model in mice [J]. Chinese national folk medicine, 2011, 20(015):52-52.
2. Zhu Zhide, Wang Zhenchang. Experimental study on liver-protecting and anti-fibrosis and anti-pathogenic effects of Xiaojin Yigan tablet [J]. Shi Zhen, Chinese medicine, 2012(6).
3. Yan Yinhong, Lei Xiaoyi, Fu Yihua. Effect of diammonium glycyrrhizinate enteric-coated capsules combined with Bifidobacterium tetravaccine on inflammatory indexes and liver injury in patients with NAFLD [J]. China Modern Applied Pharmacy, 2018,35(11):1713-1718.
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use: This product is for scientific research only, not for other purposes.
storage condition: 2-8 ℃, protected from light for 6 months
Note: absorbance at 505nm was detected by microplate reader, AST was used to catalyze the transamination reaction between aspartic acid and α-ketoglutarate.
dinitrophenylhydrazine reacts with α-keto acid to produce dinitrophenylhydrazone. The absorption spectra of dinitrophenylhydrazone are different under alkaline conditions, and the maximum difference is detected at 500-520nm by microplate reader, the amount of pyruvate produced was calculated as an equimolar concentration, and the activity of the enzyme was calculated. 100T the test kit can detect 50 samples (without standard), the kit is only used in the field of scientific research, not for clinical diagnosis or other purposes.

天门冬氨酸氨基转移酶(AST)检测试剂盒(赖氏微板法) - Introduction

Transaminase is a group of enzymes that catalyze the amino conversion reaction between α-amino acid and α-keto acid. Aspartate aminotransferase (AST), formerly known as glutamic oxaloacetic transaminase (GOT), mainly exists in myocardium, skeletal muscle, Liver, with the highest content of myocardium, followed by liver. AST can catalyze the amino transfer of aspartic acid and a-ketoglutarate to form glutarate and oxaloacetic acid.
Last Update:2022-10-16 17:36:19
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天门冬氨酸氨基转移酶(AST)检测试剂盒(赖氏微板法)
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