guanyl-specificrnase
Rnase T1
CAS: 9026-12-4
Molecular Formula:
guanyl-specificrnase - Names and Identifiers
guanyl-specificrnase - Physico-chemical Properties
| Molar Mass | 11.2kDa，单体 |
| Appearance | ammonium sulfate suspension |
| Storage Condition | 2-8°C |
| MDL | MFCD00132191 |
| Use | Removal of RNA from DNA extract; RNA sequencing; Ribonuclease protection analysis, synergistic effect with RNase A; Removal of RNA from recombinant protein extract; Detection of RNA transcript level synthesized by in vitro transcription of G-less cassette DNA template |
guanyl-specificrnase - Risk and Safety
| WGK Germany | 3 |
| RTECS | RF0753000 |
| FLUKA BRAND F CODES | 10 |
guanyl-specificrnase - Introduction
Ribonuclease T1 is an endonuclease that specifically degrades single-stranded RNA at the G residue site. This enzyme cleaves the phosphodiester bond between the 3 ′-guanosine residue and the adjacent nucleoside 5 ′-OH group by forming a nucleoside 2 ′,3 ′-cyclic phosphate intermediate. The reaction product is a 3 ′-GMP terminal oligonucleotide. RNase T1 exerts activity without the participation of metal ions.
Last Update：2022-10-16 17:40:59
guanyl-specificrnase - Vitality definition
1 activity unit is the amount of enzyme required to increase the 260nm wavelength absorbance of yeast RNA by 1.0 under the conditions of 37 ℃,pH7.5 for 15 minutes
Last Update：2023-08-16 21:32:38
guanyl-specificrnase - Source
E. Coli cells contain the cloned gene rntA from Aspergillus oryzae
Last Update：2023-08-16 21:32:38
guanyl-specificrnase - Inhibitors
Metal ions Mg2 +, Ca2 +, Zn2 +, Fe2 +, Cu2 +(MgCL2, 100mM concentration inhibition efficiency of about 40%;CaCL2,10mm concentration inhibition efficiency of about 30%;Zn2 +, strong inhibitory effect of Fe2 + and Cu2 + at 1mm), single nucleotide (2-GMP,3-GMP, etc.), guanilyl-2-5-guanosine
Last Update：2023-08-16 21:32:38
guanyl-specificrnase - Enzyme activity assay mixture
50mm Tris-HCL(PH7.5), 2mm EDTA, 3 mg/mL yeast RNA
Last Update：2023-08-16 21:32:38
guanyl-specificrnase - Reference Information
| introduction | ribonuclease T1 (mitrozyme) is an endonuclease of ribonucleic acid. Ribonuclease T1 (mitterase) specifically attacks the phosphate group on the 3 ′ side of guanylate, cleaves the 5 ′-phosphate bond of the adjacent nucleotide, and the final product is 3 ′-guanosine phosphate terminal oligonucleotide or 3 ′-guanosine phosphate. |
Last Update：2024-04-09 20:49:11
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Featured ProductsSpot supply
Product Name: Ribonuclease T1 from Aspergillus oryzae(Chromatographically Purif.) Visit Supplier Webpage Request for quotationCAS: 9026-12-4
Tel: +86-18821248368
Email: Int06@meryer.com
Mobile: +86-18821248368
QQ: 495145328
WhatsApp: +86-18821248368
Spot supply
Product Name: Rnase T1 Visit Supplier Webpage Request for quotationCAS: 9026-12-4
Tel: 609-228-6898
Email: sales@medchemexpress.com
tech@medchemexpress.com
Mobile: 609-228-6898
Spot supply
Product Name: Ribonuclease T1 from Aspergillus oryzae(Chromatographically Purif.) Visit Supplier Webpage Request for quotationCAS: 9026-12-4
Tel: +86-400-900-4166
Email: product@acmec-e.com
Mobile: +86-18621343501
QQ: 2881950922
Wechat: 18621343501
WhatsApp: +86-18621343501
Multiple Specifications
Product Name: Rnase T1 Visit Supplier Webpage Request for quotationCAS: 9026-12-4
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
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