endodeoxyribonucleasehindiii - Names and Identifiers
endodeoxyribonucleasehindiii - Physico-chemical Properties
Appearance | Liquid |
Storage Condition | −20°C |
endodeoxyribonucleasehindiii - Risk and Safety
endodeoxyribonucleasehindiii - Introduction
(or Hind III) is a restriction endonuclease that belongs to the family of nucleases. It is derived from E. coli.
Can recognize DNA sequences as 5 '-AAGCTT-3' and cut DNA strands between the sequences. It cuts the base pair in both the 5 'and 3' directions, generating protruding sticky ends. Because the cleavage site is highly specific, it is widely used in molecular biology research.
The main uses of
include:
1. Production and purification of DNA fragments: It can be used to cut DNA and produce DNA fragments of a specific size. These fragments can be further applied to cloning, sequencing, PCR, etc.
2. DNA recombination and connection: can be used to connect different DNA fragments for recombination experiments.
The preparation method of
is usually obtained by Escherichia coli culture and purification process. The detailed preparation method can refer to the relevant literature or buy some commercial kits immediately.
In the experimental operation, the use of any restriction enzyme requires caution to avoid possible contamination and harm. Some general safety considerations include:
1. In the process of preparation, storage and use, we should strictly abide by the relevant laboratory safety regulations and operating procedures.
2. Avoid direct contact with the solution to prevent skin and eye irritation and damage. In case of contact, immediately flush the affected area with sufficient water.
3. according to the correct container and storage conditions to avoid the damage of freeze-thaw cycle and thermal stability.
4. when using, must comply with the safety operation regulations, and according to the use of experimental equipment appropriate selection of appropriate personal protective equipment (such as experimental gloves, protective glasses, etc.).
5. when dealing with used consumables, waste treatment shall be carried out in accordance with relevant regulations.
Last Update:2024-04-09 02:00:55