VINNHWDBTRUVKY-UHFFFAOYSA-N

VINNHWDBTRUVKY-UHFFFAOYSA-N - Names and Identifiers

Name	Tetramethylrhodamine-5-iodoacetamidedihydroiodide(5-TMRIA)
Synonyms	VINNHWDBTRUVKY-UHFFFAOYSA-N Tetramethylrhodamine-4-iodoacetamide Tetramethylrhodamine-5-iodoacetamide dihydroiodide Tetramethylrhodamine-5-iodoacetamidedihydroiodide(5-TMRIA) 5-TMRIA [TetraMethylrhodaMine-5-IodoacetaMide Dihydroiodide] 9-[2-Carboxy-4-[(2-iodoacetyl)amino]phenyl]-3,6-bis(dimethylamino)xanthylium inner salt
CAS	114458-99-0

VINNHWDBTRUVKY-UHFFFAOYSA-N - Physico-chemical Properties

Molecular Formula	C26H24IN3O4
Molar Mass	569.4
In vitro study	Tetramethylrhodamine-5-iodoacetamide (0.5, 1.0, 2.0, 3.0, 4.0, 5.0, and 7.5 mM dye) shows different results for labeling carbonic anhydrase and myoglobin. At lower dye concentrations (up to 40:1 dye:thiol ratio) fluorescence labeling of only the carbonic anhydrase band is observed. However,as concentration increases, fluorescence labeling of both carbonic anhydrase and the lower myoglobin band is observed, indicating nonspecific labeling at these dye:thiol ratios.

VINNHWDBTRUVKY-UHFFFAOYSA-N - References

biological activity

Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) is a selective thiol-reactive dye, for non-specific labeling of proteins by cysteine residues. Tetramethylrhodamine-5-iodoacetamide (5-TMRIA) can be used to covalently label DNA fragments.

Last Update：2024-04-10 22:29:15

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