SD/-Trp/-Ade Broth (SD/-Trp/-Ade medium) is a medium used for yeast cell culture. SD(Synthetic Dropout) is an abbreviation for synthetic deletion medium in which specific nutrients are deleted for the purpose of screening or selecting specific yeast mutants.
SD/-Trp/-Ade Broth lacks tryptophan (Trp) and adenine (Ade), which means that only in the presence of these two nutrients can yeast cells grow normally. Therefore, by adding SD/-Trp/-Ade medium, mutant strains that cannot be synthesized or require additional supplementation of tryptophan and adenine can be selected.
The method of preparing SD/-Trp/-Ade medium is as follows:
1. Add an appropriate amount of protein source (such as yeast protein hydrolysate) and carbon source (such as glucose) to the water, and adjust the pH value.
2. Add a mixture of creatine and amino acid deletions, including deletions of tryptophan (Trp) and adenine (Ade).
3. Use distilled water to adjust the volume and autoclave at 121 degrees Celsius.
SD/-Trp/-Ade medium is widely used in yeast genetics. It can be used to test the growth ability of yeast mutants and screen for strains with specific mutations. In addition, it can also be used to perform transformation experiments with adenine gene sequences to evaluate transformation efficiency.
When using SD/-Trp/-Ade medium, you need to pay attention to the following safety information:
1. It is necessary to follow the basic safety procedures of the laboratory, including wearing laboratory gloves and goggles.
2. During preparation and use, care should be taken to avoid direct contact with the culture medium to prevent irritation to the skin and eyes.
3. When treating and disposing of waste liquid, the waste liquid should be treated and disposed according to the laboratory regulations.