Molecular Formula | C15H27N5O5 |
Molar Mass | 357.41 |
Density | 1.44±0.1 g/cm3(Predicted) |
Melting Point | >170°C (dec.) |
Water Solubility | Soluble in DMSO (25mg/ml), or water (20mg/ml) |
Solubility | Soluble in DMSO and water. |
Appearance | White powder. |
Color | White or off-white |
BRN | 1405664 |
pKa | 3.16±0.40(Predicted) |
Storage Condition | 2-8°C |
Stability | Stable for 1 year from date of purchase as supplied. Solutions in 1 |
Sensitive | Light Sensitive |
Refractive Index | 1.617 |
MDL | MFCD00080261 |
Use | This product is for scientific research only and shall not be used for other purposes. |
In vitro study | E-64 rapidly inhibited the cysteine proteases cathepsin B,H ,L, and papain, whereas it had no effect on serine proteases or metalloproteases. E-64, a specific inhibitor of cysteine proteases, effectively blocks ovarian cancer cell invasion in vitro. In addition, E-64 also exhibited in vitro antiparasitic activity against Giardia lamella decapsulation. E-64 improves the pre-implantation development of somatic cell nuclear transfer embryos, which shows another important effect of E-64. |
In vivo study | E-64 caused a significant reduction in the number of spontaneous metastases in M5076 tumor mice and had no effect on the formation of experimental metastases. E-64 also showed antiparasitic activity against mice infected with Giardia lamellae. |
Safety Description | S22 - Do not breathe dust. S24/25 - Avoid contact with skin and eyes. |
WGK Germany | 3 |
RTECS | RR0390000 |
FLUKA BRAND F CODES | 10 |
HS Code | 29252900 |
biological activity | E-64 is a non-reversible, selective cysteine protease inhibitor, also inhibit papain, calpain and cathepsin B, H, but does not inhibit serine or aspartic proteases. The IC50 for inhibition of papain was 9nm. E-64 can induce oxidative stress and apoptosis in filarial parasites. |
Target | Value |
Cysteine protease (Cell-free assay) | 9 nM |
Use | E-64 is an effective ligand for affinity purification of cysteine proteases. When coupled to a thiolated affinity matrix, binding is no longer irreversible, but retention of specificity |