CA-5F - Names and Identifiers
CA-5F - Physico-chemical Properties
Molecular Formula | C24H24N2O3
|
Molar Mass | 388.46 |
Solubility | Soluble in DMSO |
Storage Condition | -20℃ |
Use | CA-5f is a potent late-stage macroautophagy/autophagy inhibitor via inhibiting autophagosome-lysosome fusion. |
Target | Autophagy |
In vitro study | CA-5f (0-40 μM, 6 hour) concentration- and time-dependently elevates the level of LC3B-II (a marker to monitor autophagy) and SQSTM1 protein both in A549 cells and HUVECs. CA-5f (20 μM, 6 hours) inhibits the degradation of autophagosomes when treated alone or in combination Bafilomycin A1 (100 nM) or Chloroquine (30 μM) in A549 cells and HUVECs. CA-5f (20 μM) neither impairs the hydrolytic function nor the quantity of lysosomes. CA-5f (20 μM, 96 hours) inhibits the growth of A549 cells, and less cytotoxic to normal HUVECs. Cell Viability Assay Cell Line: A549, HUVECs Concentration: 20 μM Incubation Time: 96 hours Result: Exhibited more cytotoxicity against A549 cells compared with normal HUVECs. Western Blot Analysis Cell Line: A549 cells and HUVECs Concentration: 0-40 μM Incubation Time: 6 hours Result: Elevated LC3B-II (a marker to monitor autophagy) and SQSTM1 protein levels in a concentration- and time-dependent manner. |
In vivo study | CA-5f (40 mg/kg, i.p., every 2 days for up to 30 days) is well tolerated, and potently inhibits the growth of tumor in nude mice bearing A549 lung cancer cells. CA-5f (40 mg/kg, i.p.) suppresses autophagic flux and induces apoptosis in nude mice bearing A549 lung cancer cells. Animal Model: Nude mice bearing A549 lung cancer cells Dosage: 40 mg/kg Administration: Injected via caudal vein, every 2 days for up to 30 days Result: Significantly suppressed tumor volume and weight in mice, increased the number of apoptotic cells in mice. |
CA-5F - Reference
Reference Show more | 1. Zhang L, et al. Identification of compound CA-5f as a novel late-stage autophagy inhibitor with potent anti-tumor effect against non-small cell lung cancer. Autophagy. 2019 Mar;15(3):391-406. |
CA-5F - Preparation solution concentration reference
| 1mg | 5mg | 10mg |
---|
1 mM | 0 ml | 0 ml | 0 ml |
5 mM | 0 ml | 0 ml | 0 ml |
10 mM | 0 ml | 0 ml | 0 ml |
5 mM | 0 ml | 0 ml | 0 ml |
Last Update:2024-01-02 23:10:35
CA-5F - Cell Experiment
A549 cells or HUVECs were seeded into 100-mm culture dishes. When cells reached 80% confluency, they were exposed to DMSO or CA-5f for 6 h or 24 h. Then the cells were fixed, dehydrated, embedded, sectioned, and stained as previously described. Finally, the ultrathin sections of these samples were observed under a JEM-1230 transmission electron microscopy.
Last Update:2023-08-16 21:32:38
CA-5F - Animal Experiment
Four-week-old BALB/c nude mice were purchased from Beijing Vital River Laboratory Animal Technology Co., Lt and kept in specific pathogen-free conditions at the Experimental Animal Center in the Institute of Medicine in Zhengzhou University. Nude mice underwent subcutaneous injection of A549 cells (1 × 10^6) at the right scapula. 8 days after A549 cell implantation, mice exhibited palpable tumors, then they were randomly divided into 2 groups (each group n = 5). CA-5f was injected via caudal vein every 2 days for up to 30 days. DMSO was used as a control. From the initial injection, the tumor size was measured every 2 days. Tumor volumes were calculated as tumor length × (square of width)/2. At the end of the experiment, all mice were sacrificed and the tumors were resected immediately and photographed. The weight of each tumor was measured.
Last Update:2023-08-16 21:32:38
CA-5F - Introduction
CA-5f is a chemical compound, its full name is N-fluoromalimide. The following is a description of the nature, use, preparation and safety information of the CA-5f:
Nature:
CA-5f is a colorless to yellowish solid with a special pungent odor. It is stable at room temperature and insoluble in water, but soluble in organic solvents such as alcohols and ethers. It is a relatively reactive fluorine compound.
Use:
CA-5f are commonly used as fluorination reagents and fluorination media in organic synthesis. It can be used for the fluorination of carboxylic acids and ketones, and is widely used in the manufacturing process of drugs, pesticides, dyes and chemicals. In addition, CA-5f can also be used as solvents and catalysts in organic synthesis reactions.
Preparation Method:
The preparation method of CA-5f usually involves the reaction of sulfur pentafluoride (SF5Cl) with aminopyruvate to form a fluorine-containing compound, which is converted to CA-5f by hydrolysis under acidic conditions.
Safety Information:
CA-5f is an organic fluorine compound, which is corrosive and toxic. Wear appropriate protective equipment, such as gloves and glasses, to avoid contact with skin and eyes. In order to prevent inhalation of its particles or gaseous form, it should be used in a well-ventilated place. In addition, the CA-5f should be stored in a sealed container, away from the fire and oxidant to prevent it from decomposing, burning or aggravating the reaction. When handling and disposing of CA-5f, follow relevant safety guidelines and regulatory requirements.
Last Update:2024-04-09 20:52:54