Appearance | buffered aqueous glycerol solution |
Color | colorless |
Storage Condition | -20°C |
MDL | MFCD00166026 |
Hazard Symbols | Xi - Irritant |
Risk Codes | R36/37/38 - Irritating to eyes, respiratory system and skin. R43 - May cause sensitization by skin contact |
Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36 - Wear suitable protective clothing. S24/25 - Avoid contact with skin and eyes. S36/37 - Wear suitable protective clothing and gloves. |
WGK Germany | 3 |
FLUKA BRAND F CODES | 10 |
TSCA | Yes |
Reference Show more | 1. Tian, Y, Shen, L, Li, F, Yang, J, Wan, X, Ouyang, M. Silencing of RHEB inhibits cell proliferation and promotes apoptosis in colorectal cancer cells via inhibition of the mTOR signaling pathway. J Cell Physiol. 2019; 235: 442– 453. https://doi.org/10.1002/ 2. Liang, Tian-Song, et al. "MicroRNA-506 inhibits tumor growth and metastasis in nasopharyngeal carcinoma through the inactivation of the Wnt/β-catenin signaling pathway by down-regulating LHX2." Journal of experimental & clinical cancer research 38.1 (2019) 3. Shao, Yuan, et al. "Contribution of microRNA-30d to the prevention of the thyroid cancer progression: mechanism and implications." (2020).10.21203/rs.3.rs-25018/v1 4. Yue, Rong-Chuan, et al. "Calpain silencing alleviates myocardial ischemia-reperfusion injury through the NLRP3/ASC/Caspase-1 axis in mice." Life sciences 233 (2019): 116631.https://doi.org/10.1016/j.lfs.2019.116631 5. 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[IF=3.533] Liang-Liang Chen et al."SDC4 Gene Silencing Favors Human Papillary Thyroid Carcinoma Cell Apoptosis and Inhibits Epithelial Mesenchymal Transition via Wnt/β-Catenin Pathway."Mol Cells. 2018 Sep 30; 41(9): 853–867 10. [IF=3.098] Zhi‑Qiang Zhou et al."Adipose extracellular matrix promotes skin wound healing by inducing the differentiation of adipose‑derived stem cells into fibroblasts."Int J Mol Med. 2019 Feb;43(2):890-900 11. [IF=2.991] Luo Xiao-Yan et al."OSMR gene effect on the pathogenesis of chronic autoimmune Urticaria via the JAK/STAT3 pathway."Mol Med. 2018 Dec;24(1):1-13 12. [IF=2.939] Xue Yu-Long et al."Silencing of STAT4 Protects Against Autoimmune Myocarditis by Regulating Th1/Th2 Immune Response via Inactivation of the NF-κB Pathway in Rats."Inflammation. 2019 Aug;42(4):1179-1189 13. 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[IF=4.06] Xianghui Zhang et al."MiR-199a-5p inhibition protects cognitive function of ischemic stroke rats by AKT signaling pathway."Am J Transl Res. 2020; 12(10): 6549–6558 22. [IF=3.996] Zhiyan Ruan et al."Shikonin-mediated PD-L1 degradation suppresses immune evasion in pancreatic cancer by inhibiting NF-κB/STAT3 and NF-κB/CSN5 signaling pathways."Pancreatology. 2021 Apr;21:630 23. [IF=3.673] Qin Liu et al."Downregulation of microRNA-196a inhibits stem cell self-renewal ability and stemness in non-small-cell lung cancer through upregulating GPX3 expression."Int J Biochem Cell B. 2019 Oct;115:105571 24. [IF=3.448] Rong-Chuan Yue et al."Calpain silencing alleviates myocardial ischemia-reperfusion injury through the NLRP3/ASC/Caspase-1 axis in mice."Life Sci. 2019 Sep;233:116631 25. [IF=3.911] Wu Gang et al."Overexpression of ORX or MCH Protects Neurological Function Against Ischemic Stroke."Neurotox Res. 2022 Jan;:1-12 |
EPA chemical information | Information provided by: ofmpub.epa.gov (external link) |
introduction | DNA polymerase is an important enzyme for cell replication of DNA. DNA polymerase, using DNA as a replication template, replicates DNA from the 5'end point to the 3'end enzyme. The main activity of DNA polymerase is to catalyze the synthesis of DNA (in the case of templates, primers, dNTP, etc.) and its phase-assisted activity. |
Function | DNA polymerase I can extend the newly synthesized DNA chain along the DNA template and along the primer of the RNA component. 3 '-5' exonuclease can correct for unsuccessfully paired deoxynucleotides. With the continuous replication of DNA, the downstream RNA primers gradually approach the polymerization active site. In the end, RNA primers will break away from the DNA template and form flapDNA. Under the action of thermal fluctuations, the flapDNA will detach from the polymerase region; at the same time, the 5'nuclease region deviates from the equilibrium position under the action of thermal movement, stretches the linker, and gradually moves close to the polymerase region, and finally the 5'nuclease The active site will capture the flapDNA near the active site of the polymerase and remove the RNA primer, leaving a small gap, which will be repaired by DNA ligase. |