Molar Mass | 38KDa。 |
Density | 100g/mLat 20°C |
Water Solubility | 125g/L at 25℃ |
Solubility | Soluble in water, slightly soluble in 30% ethanol and acetone. |
Vapor Presure | 0.004Pa at 25℃ |
Appearance | solution (clear, colorless) |
Color | white |
Merck | 13,2923 |
Storage Condition | -20°C |
MDL | MFCD00130918 |
Physical and Chemical Properties | White-like freeze-dried powder, soluble in water, slightly soluble in 30% ethanol and acetone, the optimum pH value is 7.8. Adding 5mmol/L Ca2 + or diisopropyl fluorophosphate (DFP) to aqueous solution can increase its stability. The inhibitors include EDTA and sodium dodecyl sulfonate; the activator is a divalent metal ion, and the combination of Mg2 and Ca2 can get the best effect. The absorption coefficient of 1% aqueous solution is 11.1 at 280nm. The average strand length that restricts digestion is tetranucleotide, and the enzymatic reaction: deoxyribonucleotide -5 '-phosphate oligonucleotide -5'-phosphate. |
Use | For molecular biology research. Various enzymes that degrade DNA. These enzymes hydrolyze the phosphodiester bond on the sugar-phosphate backbone. There are two types of such enzymes: exonuclease and endonuclease. |
Safety Description | S23 - Do not breathe vapour. S24/25 - Avoid contact with skin and eyes. S22 - Do not breathe dust. |
WGK Germany | 3 |
RTECS | RF0750000 |
FLUKA BRAND F CODES | 10-21 |
TSCA | Yes |
HS Code | 35079090 |
Reference Show more | 1. Yang Xueli, Zhang Honglei, Yang Yutao, etc. Determination of doxorubicin in DNA nano-transport system by HPLC [J]. Journal of Pharmaceutical Analysis 2019(7). 2. Weng Ya Yi Bao Wei Jie Liang Rui Min et al. Effect of MAPK signaling pathway on proliferation, invasion and migration of endometrial carcinoma cells [J]. Journal of Clinical and pathology 2019 000(003):P.470-478. 3. Feng Junye, Li Jiaqi, Wang Ping. Quality changes and volatile compounds analysis of wild Lonicera edulis during fermentation [J]. Modern food science and technology, 2018, 34(11):117-124. 4. Academic Edition) 2018 036(005):82-91 105. 5. Zhou Yue Carnation, Wang yaner et al. Therapeutic effect of ginsenoside Rb1 on Kawasaki disease in mice [J]. Journal of Traditional Chinese Medicine 2017 v.45;No.236(06):40-44. 6. [IF = 3.591] Hengchang Ma et al."Multiple cation-doped linear polymers toward ATP sensing and a cell imaging application."New J Chem. 2018 Jun;42(12):9702-9708 7. [IF=7.086] Weixiang Wang et al."Developmental toxicity and alteration of gene expression in zebrafish embryo exposed to 6-benzylaminopurine."Chemosphere. 2019 Oct;233:336 |
This strain of deoxyribose nuclear hydrolase extracted from bovine pancreas is white powder, soluble in water, and Mg2 is activator. The molecular weight was about 31000. It is a highly specific phosphodiesterase, it can cut double-stranded DNA or single-stranded DNA into 5 '-phosphate as the end of the oligonucleotide, the average length of four nucleotides, Mg2 or Mn2, c02, the optimum pH was 7~8. It only acts on extracellular substances and necrotic cells, viscous sputum and contains a large amount of DNA, this product can not only make the sputum DNA rapid lysis, and make the original DNA in sputum combined with protein loss of protection, it is easy to be digested by the proteolytic enzyme in the white blood cells, so that the viscosity decreases rapidly.
LogP | -1.3 at 20℃ |
EPA chemical substance information | information provided by: ofmpeb.epa.gov (external link) |
Introduction | DNases are a class of nucleases that hydrolyze DNA, including endonucleases (DNase I and restriction enzymes) with exonuclease (exonuclease III, VII). DNase exists widely in many mammals, shrimp, insects and plants, and has important biological functions for individual development, normal cell aging and apoptosis, and the construction of defense system. |
Application | DNase is a multifunctional endonuclease that cleaves DNA in a non-specific manner, acting on the 3 ',5'-phosphodiester bond between two deoxyribonucleotides cleaves the DNA strand and degrades it into a single nucleotide or oligonucleotide. Among them, the most studied are mammalian and human DNA enzymes. For example, DNase I derived from bovine pancreas is widely used in biological research and medical molecular detection kits, human-derived recombinant DNase I has also been used in the treatment of some diseases, such as cystic fibrosis (CF), atelectasis and complicated Pneumonia with pleural effusion and empyema. |
Use | is used for biochemical research, commonly used as expectorant in clinic, and can also be used locally for abscess, hematoma and intrathecal injection for meningitis. for the removal of DNA deoxyribosuclease I from bovine pancreas has been used in a study to investigate a two-dimensional model analysis of nucleases bacteria subtilis. Deoxyribonuclease I from bovine pancreas has also been used in a study to investigate the effects of minor and major Ucelase I. An endonuclease that acts on a phosphate dilipid bond, particularly against a bond adjacent to a pyrimidine nucleoside, thereby producing a polynucleotide with a free hydroxyl group at the 3 'end and a phosphate at the 5' end. The optimum pH value of the enzyme was 7.8. DNase can be activated by divalent metals and inhibited by chelates such as EDTA and sodium dodecyl sulfate. Calcium ion at a concentration of 5mm can be used as a stabilizer to protect DNase from being decomposed by hydrolytic enzymes. It is mainly used to degrade DNA in RNA, purify RNA, and study the interaction between DNA and protein. |
production method | using bovine pancreas as raw material, the product was obtained by Fractional precipitation of ammonium sulfate and ethanol after extraction with dilute sulfuric acid. |