Molecular Formula | C60H100N2 |
Molar Mass | 849.4494 |
Solubility | Soluble in aqueous buffers. |
Appearance | powder |
Color | light brown |
Merck | 2481 |
PH | 7.0 |
Storage Condition | -20°C |
MDL | MFCD00130830 |
Use | It has unique ability to digest natural collagen and denatured collagen. Detergents, hexachlorocyclohexane and heavy metal ions can reduce enzyme activity. It specifically acts on procollagen, breaks it and is hydrolyzed by other proteases, without hydrolyzing fibrin, globulin |
Hazard Symbols | Xn - Harmful |
Risk Codes | R36/37/38 - Irritating to eyes, respiratory system and skin. R42 - May cause sensitization by inhalation |
Safety Description | S22 - Do not breathe dust. S24 - Avoid contact with skin. S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36/37 - Wear suitable protective clothing and gloves. |
WGK Germany | 1 |
FLUKA BRAND F CODES | 10-21 |
HS Code | 3507907000 |
Reference Show more | 1. Deng Xuyang, Fang, Tang Xiqing, et al. miR-20 regulates directional differentiation of human deciduous dental pulp stem cells into neuron-like cells [J]. Chinese Journal of Tissue Engineering Research, 2019, 23(21):55-61. 2. Yang Hui, Su, Yujiang, Zhong, Jiang Hua et al. MiR-506-3 p targeting TRAF6 inhibits inflammatory response and cardiomyocyte apoptosis in myocarditis [J]. Chinese Journal of Gerontology 2019(19). 3. Li Qiaozhi, Wang Zhentao, Wu Hong, et al. Effects of different digestion methods on cardiac fibroblasts cultured in vitro [J]. Journal of Liaoning University of Traditional Chinese Medicine 2020 v.22;No.192(04):42-47. 4. Ji Xian, Yan Gao, Yi Yi Ye, Zhang Lei, Cao, Jiang Su, Hu Jia-Bo. Effects of human embryonic stem cell-derived neurosphere conditioned medium on proliferation and migration of rat Schwann cells [J]. Journal of Jiangsu University (Medicine edition) 2019 29(03):189-194. 5. Tu Haiyan, Liu Wuzhou, Wu Guotai, Gao Yunjuan, Ren Yuan. Uniform design for isolation of single gastrointestinal smooth muscle cells in rats [J]. Journal of Gansu College of Traditional Chinese Medicine, 2015,32(04):1-5. 6. Zhang Hui, Wang Yixiang, Qiu Yinger, etc. Effects of different components of Tripterygium wilfordii column chromatography on cytokine expression in collagen-induced arthritis rats [J]. Chinese Journal of Modern Applied Pharmacy, 36(19). 7. Zhang Yufang, Wang Qian, Yang Lei, etc. Effects of combined extract of astragalus and Salvia miltiorrhiza on energy metabolism of injured myocardial cells [J]. Science Technology and Engineering, 2019, v.19;No.498(29):70-75. 8. Liao Zhihong, Chen Yanyan, Huang, Xiaoying, et al. Anti-hepatic fibrosis effect of total saponins from fruit-splitting potato and its mechanism in rats [J]. Chinese pharmacological Bulletin, 2019, 35(05):42-47. 9. Wang Huafu, Gui Zhihong, Zhang Xiaoru, etc. Pharmacokinetics of Liuwei Dihuang pill in diabetic rats with kidney yin deficiency by microdialysis [J]. Northwest Pharmaceutical Journal, 2019, 034(001):87-90. 10. Huang Ting, Chen Bo, Zeng Zhaoming. Effect of VEGF gene modified human bone marrow mesenchymal stem cells on early brain edema in rats with intracerebral hemorrhage [J]. Journal of Sichuan University (Medical Sciences),2020,51(05):622-629. 11. Wang Boyan, Wu Hongyan, Cai Huili, Zhou Yaojun, Huang quanchuan, Jia Siyu, Chen Haidan. Construction of rat neural cell model with high expression of β-1,6 acetylglucosamine branched chain [J]. Bachu medical journal, 2020,3(04):11-17. 12. Li, Wu Chunlin, Deng Xiaoyan, Hu Yajun. Expression of IFITM 1 and IFITM 3 in endometriosis [J]. China Family Planning & Obstetrics & Gynecology, 2020,12(09):22-26. 13. Lüs, Shawa, et al. "Metabolonic Analysis of the Urine from Rats with Collagen-Induced Arthritis with the Effective Part of caulophylum robustum Maxim." Evidence-Based Complete and Alternative Medicine 2021 (2021).https://doi.org/10.1155/2021/5580341 14. [IF = 10.317] Xuemin Liu et al."Molecular recognition-directed site-specific release of stem cell differentiation inducers for enhanced joint repair."Biomaterials. 2020 Feb;232:119644 15. [IF=12.479] Yunjian Yu et al."Polymeric PD-L1 blockade nanoparticles for cancer photothermal-immunotherapy."Biomaterials. 2022 Jan;280:121312 |
This product is ointment containing collagenase (0.5%, 50 u/mg), neomycin 1%, Vaseline 9.85%. Collagenase is produced by Clostridium histolytica and has a unique ability to digest natural collagen and denatured collagen. The optimum pH was 7~8, and the enzyme activity could be reduced by detergent, HCH and heavy metal ions. It can hydrolyze the collagen of the necrotic tissue in the center and marginal zone of the scald, make the burn surface covering and eschar separate and dissolve in 24~96H, and has therapeutic effect on chronic ulcer, bedsore and burn, and 90% of the necrotic tissue digestion. It is superior to other proteases. It does not hydrolyze fibrin, globulin, and thus does not harm tissues and organs such as blood vessels and nerves.
occasional mild erythema. To prevent cleaning agents, Six Six Six powder and heavy metal ions to reduce enzyme activity.
EPA chemical substance information | information provided by: ofmpeb.epa.gov (external link) |
protease | Collagenase, also known as Collagenase and Clostridial peptidase, it is a protease derived from the fermentation of Clostridium histolytica. Medical collagenase is refined from the culture medium of Clostridium histolytica atcc21000. This enzyme has a high decomposition activity on the peptide bond of the gly-pro sequence. Due to the cleavage of procollagen molecules, it provides good conditions for the hydrolysis of other proteases, so that it has a unique function of hydrolyzing collagen and denaturing collagen. The medical enzyme prepared by the enzyme has the ability to digest natural collagen and denatured collagen, has a strong digestive effect on necrotic tissue, and can promote the growth of granulation and epithelial cells. This product has no effect on globulin and fibrin, so it does not affect the normal blood vessels and muscle tissue of human body. Can be used for the treatment of II, III degree burn debridement, removal of scab and skin chronic ulcers, bedsores and so on. [usage and dosage] first, perform surgical debridement, clean the surrounding skin and wound with physiological saline and alcohol cotton ball, then apply 0.1cm thick ointment and bake for 20 minutes, to accelerate the enzyme activity and diffusion, enhance the penetration of the drug, and then wrapped with Disinfection gauze. [note] 1. This product should be used before the use of antimicrobial drugs to control infection. Detergent, heavy metal ions can reduce the enzyme activity. |
collagen | collagen is also called collagen. A fibrous protein that is twisted into a helix by three peptide chains. It exists in the form of collagen fibers in the body and is dissolved by heating in water to form a gel. In the body of higher animals, the content of protein is rich, accounting for about 1/3 of the total amount of protein. It is mainly distributed in connective tissues, for example, 70% of the dry weight of skin is collagen. Collagen is a glycoprotein, the molecule contains sugar, and a lot of glycine, proline and Hydroxyproline, without cystine and tryptophan. Incomplete protein, nutritional value is not high, but the animal and human skin, blood vessels, bones, tendons, teeth and cartilage formation is very important, is the main matrix of these connective tissue. If the biosynthesis of collagen is abnormal, or the collagen is abnormally changed for other reasons, it may lead to collagen disease. In clinical medicine, the occurrence of collagen diseases such as rheumatoid arthritis and lupus erythematosus is related to abnormal collagen metabolism. Industry can be used skin, bone as raw material preparation of impure collagen, such as Kraft glue, fish gelatin and bone glue and other industrial glue, for bonding. Donkey skin made of donkey gelatin, is an important tonic, blood function. Gelatin (also known as gelatin) is pure collagen, generally also skin, bone as raw material, after acid hydrolysis, and then water at 60 degrees in the extraction. Gelatin can be used for food or to prepare photographic latex, and can also be used to extract hydroxyproline. |
collagen molecular structure | The monomer of collagen is procollagen. The procollagen molecule has a relative molecular mass of 300,000, a fine rod shape, a length of about 30nm and a diameter of 1.5nm. It is a string-like right-hand composite Helix twisted by three left-hand helix α-peptide chains. The polypeptide chain constituting the procollagen molecule includes an α1 chain and an α2 chain. Alpha 1 chain and there are four forms: Alpha 1 (I), alpha 1 (II), alpha 1 (III), alpha 1 (IV). Thus, there are actually five kinds of α-peptide chains constituting the procollagen molecule. The amino acid composition of the five alpha peptide chains is basically similar, but the order is different. The five alpha peptide chains described above constitute four different types of procollagen molecules. Figure 1 shows the alpha peptide chain composition of different types of procollagen molecules. each α-peptide chain of collagen is composed of more than 1000 amino acid residues, and has the following characteristics: ① glycine residues account for about 1/3. ② proline and hydroxyproline accounted for about 1/5, of which proline was about 12% and hydroxyproline was about 9%. (3) in the α-peptide chain, the amino acids of the main segments presented (Gan-X-Y)n periodic arrangement. (4) the acidic and basic amino acid residues are about 1/4, and their distribution is relatively concentrated. |
biosynthesis of procollagen molecules | The biosynthesis of procollagen molecules is divided into two stages; in the rough endoplasmic reticulum of cells, the procollagen molecules are biosynthesized and secreted out of the cells. In the extracellular procollagen molecules, the N-terminal and C- terminal regions are excised by endopeptidases, which are procollagen molecules. FIG. 2 is a schematic diagram of the biosynthesis of procollagen molecules. FIG. 3 is a schematic diagram of collagen fiber formation process. |
Properties of collagen | collagen is a whitish, hard and brittle substance in the absolute state, with a relative density of 1.4. collagen is insoluble in cold water and organic solvents. collagen is an amphoteric electrolyte, and the isoelectric point of bovine collagen is 7.5-7.8. At the isoelectric point, collagen swelling is minimal. Collagen has an acid capacity of 0.82 to 0.9mmol (monovalent acid or 1/2 divalent acid) and a base capacity of 0.4 to 0.5mmol (monovalent base or 1/2 divalent base). collagen undergoes gelation and hydrolysis in acid-base solution. Calcium hydroxide has the greatest ability to gel. The ability of strong acids to gel is greater than that of strong bases. If the concentration of strong acid or strong base is large or the treatment time is too long, the collagen is hydrolyzed. Leather-making process requires that the collagen is moderately gelatinized, which is beneficial to the loosening of the collagen fiber structure; Do not want excessive hydrolysis of collagen, otherwise excessive loss of cortex, affecting the quality of leather. collagen only slightly swells in a dilute solution of table salt. When the salt concentration is high, dehydration can be induced, and the contraction temperature of collagen is slightly increased. Sulfate, thiosulfate and carbonate can cause collagen dehydration at any concentration, but the adsorption is small. Collagen in any concentration of calcium salt, magnesium salt, barium salt will produce a sharp expansion, fiber bundles Shorten, become thick, its contraction temperature is also significantly reduced. In the process of leather production, the application of neutral salt in the process of soaking, dehydration, pickling and so on. some proteases can hydrolyze collagen, such as chymotrypsin, pepsin, papain, collagenase, etc. Trypsin acts only on denatured collagen. The specificity of trypsin is to hydrolyze the peptide bonds at the C- terminus of lysyl and arginyl. The most suitable conditions for the hydrolysis of denatured collagen are: pH 8.1~8.2, temperature 37. When collagen fibers are heated to a certain temperature in water, they will shrink and curl themselves. At this time, the temperature of the water that produces the deformation of collagen fibers is called the shrinkage temperature, which is expressed by Ts. |
efficacy and function of collagen | (1) collagen plays an important role in wound healing and scar formation, it can not only serve as a prerequisite for elastic tissue and adhesion substances, but also stimulate cell development, differentiation and stimulation of tissue differentiation and proliferation and stimulation of connective tissue differentiation and proliferation, but also stimulate capillary neogenesis, chemotaxis of monocytes and fibroblasts, nutrition and regulation of granulation tissue. It is reported that the treatment of skin ulcers caused by different reasons with collagen shows good curative effect, which can make the ulcer surface and deep epithelium, and is conducive to the formation of granulation tissue. (2) 70%-80% of the organic matter in bone is Collagen. When bone is formed, sufficient collagen fibers must be synthesized to form the framework of bone. Therefore, some people call collagen the bone in the bone. Collagen fibers have strong toughness and elasticity, if a long bone is compared to a cement column, then the collagen fibers are the reinforced frame of this column, and the lack of collagen, just like the use of low-quality steel bars in buildings, the risk of breaking is always present. (3) the effect of collagen on breast enhancement is well known. The breast is mainly composed of connective tissue and adipose tissue, and the straight and plump breast depends largely on the support of connective tissue, collagen is the main component of connective tissue," In connective tissue, collagen and polyglycoproteins are often interwoven into a network structure, resulting in a certain degree of mechanical strength, which is the material basis for supporting the human body curve and reflecting the posture. " (4) collagen is called" bone in the bone, skin in the skin, meat in the meat ", can be said to be a strong backing of the dermis, its effect on the skin is self-evident. Protection, with appropriate elasticity: the lower layer of the epidermis, which occupies most of the structure is the dermis layer, the thickness is about 2mm, and can be divided into three layers, namely the papillary layer, the lower layer of the nipple and the reticular layer, etc, most of them are composed of proteins, which are the same as collagen and elastin (elastin), and others are nerves, capillaries, sweat glands and sebaceous glands, lymphatic vessels and hair roots. 70% of the skin composition is composed of collagen, skin such as a large set of tightly wrapped around the body, the surface area is quite large, the human limbs, the skin collagen function, so that the skin has a protective function, and have appropriate elasticity and hardness. |
elastin | elastin is the second most abundant protein in the mammalian skin, it is also another important structural protein, which has many properties similar to those of natural rubber. It is the main protein of elastic fibers in higher animals, which can give elasticity to blood vessel walls and tendons. It also contains a small amount of elastin in skin and tendons. In recent years, hydrolyzed elastin has become one of the most popular proteins used in skin care cosmetics. at present, there are two kinds of elastin Crolastin and Elastosol on the market: Crolastin is a hydrolyzed elastin, yellow-white liquid, with a relative molecular mass of about 4000, the mass fraction of the active substance is 30%, which has film forming property and wettability. Elastosol is a soluble native elastin, actually a mixture of soluble elastin and soluble collagen. It is a white to beige mucus with a relative molecular mass of about 200000 and a mass fraction of active content of 2%. It retains the properties of hydrolyzed elastin, and has a higher sensitivity to the skin, can leave a layer of natural protein mimicking the colloidal film on the skin, so that the skin gives elasticity. It is also an effective wetting agent. hydrolyzed elastin is mainly used to increase skin flexibility, moisten skin, improve skin color and appearance, especially suitable for skin cleansers, and the typical dosage is 1% ~ 5%. Soluble natural elastin can form a colloidal film on the skin, which increases the elasticity of the skin, especially for the eyes. safety: elastin is a component of mammalian skin that is non-toxic and safe. |
production method | 1. It is prepared from Clostridium histolytica. The optimum pH value of 7~8. Has a unique ability to digest natural collagen and denatured collagen. Detergents, HCH and heavy metal ions can reduce enzyme activity. It acts exclusively on procollagen, which breaks and is hydrolyzed by other proteases, and does not hydrolyze fibrin or globulin. Mammalian skin, cartilage, fascia, blood vessels, intestines, cornea, etc. as raw materials, by enzyme hydrolysis, purification, drying and derived. |