Name | Ponceau S |
Synonyms | Ponceau S PONCEAU S C.I. 27195 Acid red 112 c.i.acidred112 FAST PONCEAU 2B C.I. Acid Red 112 ATX PONCEAU S RED Ponceau S solution LABOTEST-BB LT00847573 PONCEAU S, SODIUM SALT PONCEAU S (C.I. 27195) FOR MICROSCOPY CE C.I. Acid Red 112, tetrasodium salt (8CI) tetrasodium 3-hydroxy-4-(2-sulphonato-4-(4-sulphonatophenylazo)phenylazo)naphthalene-2,7-disulphonat 3-Hydroxy-4-[2-sulfo-4-(4-sulfophenylazo)phenylazo]-2,7-naphthalenedisulfonic acid, tetrasodium salt 2,7-Naphthalenedisulfonicacid,3-hydroxy-4-[[2-sulfo-4-[(4-sulfophenyl)azo]phenyl]azo]-,tetrasodiumsalt tetrasodium 3-hydroxy-4-[(E)-{2-sulfonato-4-[(E)-(4-sulfonatophenyl)diazenyl]phenyl}diazenyl]naphthalene-2,7-disulfonate |
CAS | 6226-79-5 |
EINECS | 228-319-2 |
InChI | InChI=1/C22H16N4O13S4.4Na/c27-22-20(43(37,38)39)10-12-9-16(41(31,32)33)6-7-17(12)21(22)26-25-18-8-3-14(11-19(18)42(34,35)36)24-23-13-1-4-15(5-2-13)40(28,29)30;;;;/h1-11,27H,(H,28,29,30)(H,31,32,33)(H,34,35,36)(H,37,38,39);;;;/q;4*+1/p-4/b24-23+,26-25+;;; |
Molecular Formula | C22H17N4NaO13S4 |
Molar Mass | 696.62 |
Density | 1.01g/mLat 20°C |
Water Solubility | 10 g/L |
Solubility | Soluble in water and ethanol, the aqueous solution is rose red |
Appearance | Bordeaux red to dark red or brown powder |
Color | Red-brown |
PH | 9.0 (10g/l, H2O, 20℃) |
Storage Condition | Store at RT. |
Stability | Stable. Incompatible with strong oxidizing agents. |
Refractive Index | n20/D 1.338 |
MDL | MFCD00003892 |
Use | Biological staining, rapid staining of protein bands after electrophoresis on cellulose acetate. Lichun Red S is used to dye proteins on nitrocellulose or PVDF membranes (Western blots). The following is the preparation of commonly used dyeing solutions: 0.1% Lichun Red S (w/v) in 5% acetic acid;2% Lichun Red S (w/v) in 30% TCA;30% sulfosalicylic acid. |
Hazard Symbols | Xi - Irritant |
Risk Codes | 36/37/38 - Irritating to eyes, respiratory system and skin. |
Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36 - Wear suitable protective clothing. S37/39 - Wear suitable gloves and eye/face protection |
WGK Germany | 3 |
RTECS | QJ6600000 |
TSCA | Yes |
HS Code | 32041200 |
Color index | 27195 |
EPA chemical substance information | information provided by: ofmpeb.epa.gov (external link) |
Overview | lichenium red is negatively charged and can bind to positively charged amino acid residues, at the same time, the red can also be combined with the non-polar region of the protein to form a red band. The staining of the protein by the Green red is reversible and can be washed off with distilled water, PBS or other appropriate solution after staining. |
biological activity | Ponceau S (Acid Red 112) is a common stain used for Western blot. Ponceau S is used in the form of an acidic aqueous solution, is compatible with antibody-antigen binding, and stains the protein on the membrane in red. |
Use | Green red S is used to stain proteins on nitrocellulose or PVDF membranes (Western blots), the following is the commonly used dyeing solution preparation: 0.1% Red S (w/v) in 5% acemic acid 2% Red S (w/v) in 30% TCA, 30% sulfosalicylic acid biostaining, rapid staining of protein bands after electrophoresis on cellulose acetate. |