Molecular Formula | C30H41FN4O12 |
Molar Mass | 668.66 |
Density | 1.260±0.06 g/cm3(Predicted) |
Boling Point | 914.2±65.0 °C(Predicted) |
Solubility | DMSO/DMF: ≥20 mM |
Appearance | White to slightly brown solid |
pKa | 10.26±0.46(Predicted) |
Storage Condition | Inert atmosphere,Store in freezer, under -20°C |
Physical and Chemical Properties | Bioactive Z-DEVD-FMK (Caspase-3 Inhibitor) is a specific and irreversible Caspase-3 inhibitor, which can also effectively inhibit caspase-6, caspase-7, caspase-8 and caspase-10. |
In vitro study | Z-DEVD-FMK (1-200 μm) inhibited D4-GDI lysis and apoptosis in a concentration-dependent manner. Z-DEVD-FMK reduced ceramide-induced cardiomyocyte death and significantly inhibited caspase 3 activation. Z-DEVD-FMK (100 μm) attenuated OxyHb-induced cell shedding in cultured brain microvascular endothelial cells, reduced activity by caspase-2 and -3, abolished OxyHb-induced DNA helices, and to prevent OxyHb induced PARP cleavage. Z-DEVD-FMK (μm) blocks the MPP-induced increase in caspase-3 enzyme activity. Z-DEVD-FMK dose-dependently blocked 6-OHDA-induced apoptosis with an IC50 of 18 μm. |
In vivo study | Z-DEVD-FMK, before and after injury, significantly reduce the apoptosis after trauma, and significantly improve the recovery of neurological function. |
WGK Germany | 3 |
1mg | 5mg | 10mg | |
---|---|---|---|
1 mM | 1.496 ml | 7.478 ml | 14.955 ml |
5 mM | 0.299 ml | 1.496 ml | 2.991 ml |
10 mM | 0.15 ml | 0.748 ml | 1.496 ml |
5 mM | 0.03 ml | 0.15 ml | 0.299 ml |
biological activity | Z-DEVD-FMK (Caspase-3 Inhibitor) is a specific and irreversible Caspase-3 inhibitor that can also effectively inhibit caspase-6, caspase-7, caspase-8 and caspase-10. |
target | TargetValue Caspase-3 |
Target | Value |
in vitro study | Z-DEVD-FMK (1-200 μM) inhibited D4-GDI lysis and apoptosis in a concentration-dependent manner. Z-DEVD-FMK reduced ceramide-induced myocardial cell death and significantly inhibited caspase 3 activation. Z-DEVD-FMK (100 μM) attenuated OxyHb-induced cell shedding in cultured brain microvascular endothelial cells, decreased caspase-2 and -3 activity, abolished OxyHb-induced DNA helix, and prevented OxyHb-induced PARP cleavage. Z-DEVD-FMK (100 μM) blocked MPP-induced increase in caspase-3 enzyme activity. Z-DEVD-FMK dose-dependent blockade of 6-OHDA-induced apoptosis with an IC50 of 18 μM. |
in vivo studies | Z-DEVD-FMK, before and after injury, apoptosis after trauma was significantly reduced, and the recovery of nerve function was significantly improved. |