| Overview | cephalin, also known as phosphatidylethanolamine. It is widely distributed in animals, especially in the brain and spinal cord, and has a high content in soybean. It can be used as a hemostatic agent and a reagent for liver function test in clinic. A phospholipid composed of glycerol, fatty acids, phosphoric acid, and ethanolamine. Fresh products are colorless solids that tend to turn red-brown in the air. Hygroscopic. Insoluble in water and acetone, slightly soluble in ethanol, soluble in chloroform and ether. It can be used as an antioxidant and also used in medical treatment. It can be extracted from fresh brain after slaughter of livestock or by-products after soybean oil extraction. |
| Use | cephalin is an excellent natural active agent, with unique biological activity and physiological function, and non-toxic, no stimulation, also will not cause pollution to the environment, can be extracted from the fresh brain of livestock slaughter or soybean oil by-products, used as antioxidants and medical. |
| Application | cephalin is a good natural surfactant, with unique biological activity and physiological function, non-toxic, non-polluting, no stimulation, easy biodegradation and high attention by the domestic and international scientific and industrial circles, at the same time, brain phospholipid as a new type of rubber vulcanization accelerator and/or antioxidant. |
| preparation | fresh egg yolk was defatted with acetone for three times, vacuum suction filtration, the filter cake was extracted twice with 95% alcohol, and the filtrate was combined, the crude phospholipid was obtained by vacuum concentration. By HPLC analysis, crude phospholipids contained 75.0%(wt%) of PC and 16.0%(wt%) of PE. Take 10% water content of silica gel with 10% methanol in dichloromethane mixed solvent wet Ф/2 inch ID x 20cm column (column volume of 25ml), first, 50ml of dichloromethane mixed solvent containing 10% of methanol was washed. Into 60ml concentration of crude phospholipid solution 100mg/ml (solvent is containing methanol 10% dichloromethane solution), followed by washing with 0.4ml methanol, flow rate control at ml/min, the effluent was collected starting after the loading (about 5ml each). The effluent was analyzed by TLC, and then the brain phospholipid fractions were combined. After vacuum concentration and freeze-drying, 0.55g of brain phospholipid was obtained, and the content of brain phospholipid in the product was 91% by HPLC analysis. The column was washed with 150ml of a methanol solution containing 2% by weight of ammonia (prepared from ammonia containing 25% by weight), then flush 100ml with dichloromethane mixed solvent containing 10% methanol, and then the sample can be re-injected, and the washing flow rate is controlled at 0.4ml/min. |