Molecular Formula | C29H60O20 |
Molar Mass | 728.77 |
Melting Point | 260 °C |
Water Solubility | Soluble in water. |
Solubility | H2O: 10 mg/mL cold |
Appearance | powder |
Color | white |
BRN | 101712 |
Storage Condition | room temp |
Stability | Stable for 2 years from date of purchase as supplied. Protect from exposure to moisture. Solutions in DMSO or distilled water may be stored at -20°C for up to 3 months. |
Sensitive | Light Sensitive |
Physical and Chemical Properties | Bioactive Ouabain Octahydrate is a Na /K -ATPase inhibitor with potential for congestive heart failure research. |
Use | Uses typical inhibitors of Na , K -ATPase, inhibiting cation transport. Cardiac glycoside. Biochemical studies |
Hazard Symbols | T - Toxic |
Risk Codes | R23/25 - Toxic by inhalation and if swallowed. R33 - Danger of cumulative effects |
Safety Description | 45 - In case of accident or if you feel unwell, seek medical advice immediately (show the label whenever possible.) |
UN IDs | UN 1544 6.1/PG 1 |
WGK Germany | 3 |
RTECS | RN3850000 |
FLUKA BRAND F CODES | 8 |
HS Code | 29389090 |
Hazard Class | 6.1(b) |
Packing Group | III |
EPA chemical substance information | Card-20(22)-enolide, 3-[(6-deoxy-.alpha.-L-mannopyranosyl)oxy]-1,5,11,14,19-pentahydroxy-, octahydrate, (1.beta.,3.beta.,5.beta.,11.alpha.)- (11018-89-6) |
in vitro studies
Ouabain (100 μM) induces NLRP3 inflammasome activation and IL-1β release in macrophages. Ouabain-induced NLRP3 inflammasome activation is mediated through K efflux. Ouabain (3 nM) alters the expression of EMT markers in NHK and ADPKD cells, and modifies cell-cell adhesion properties in ADPKD. Moreover, ouabain enhances migration of ADPKD cells selectively modulates tight junctions, and modulates adherens junctions in ADPKD cells in a selective manner. Ouabain also activates TGFβ-Smad3 signaling, alters TER in ADPKD cells. Ouabain (25, 50 or 100 nM) treatment significantly reduces cell proliferation and viability in Raji cells in a dose-dependent manner with IC 50 of 76.48±4.03 nM. Ouabain increases the number of apoptotic cells, induces autophagy, and upregulates Beclin-1 in Raji cells.
in vivo studies
Ouabain (3 mg/kg) significantly decreases cardiac contractile force with an enlarged LVESD when mice are primed with LPS. IL-1β deficiency attenuates ouabain-induced cardiac dysfunction and injury. IL-1β secreted by infiltrated macrophages contributes to ouabain-induced cardiac inflammation. Deficiency of NLRP3 and Casp1 attenuates ouabain-induced cardiac dysfunction and macrophage infiltration. Ouabain (30 µg/kg, I .p.) modulates ABCB1 activity in thymocytes of Wistar rats and it has the same effect on Swiss mice at 300 µg/kg. After 14 days of ouabain treatment, the MAP of rats is significantly elevated.
category
toxic substances
toxicity grade
highly toxic
Acute toxicity
intraperitoneal-mouse LD50: 1.213 mg/kg; Intravenous-mouse LD50: 3.750 mg/kg
flammability hazard characteristics
flammable, fire discharge spicy and stimulating smoke
storage and transportation characteristics
warehouse low temperature, ventilation, dry; Separate storage with food raw materials
fire extinguishing agent
water, carbon dioxide, dry powder, sand