中文名 | 胃蛋白酶 |
英文名 | pepsin |
别名 | 胃朊酶 胃液素 百布圣 蛋白酵素 胃蛋白酶 胃蛋白酶 A 胃液素 来源于猪胃 胃液素,胃朊酶,蛋白酵素,酸腈酶,胃酶 |
英文别名 | pepsin Pepsin A AVIANPEPSIN BOVINEPEPSINB BOVINEPEPSINA IMMOBILIZED PEPSIN Pepsin(porcine stoMach Mucose) OMniTrace Ultra (TM) Perchloric Acid |
CAS | 9001-75-6 |
EINECS | 232-629-3 |
化学式 | n.a. |
密度 | 1.287 g/cm3 |
比旋光度 | D26 -64.5° (water pH 4.6) |
水溶性 | Soluble in water and dilute aqueous buffers |
溶解度 | 10mm HCl: 可溶物1.0mg/mL,澄清至微混浊,无色 |
存储条件 | -20°C |
稳定性 | 稳定。与强碱、碱、金属盐、醇不相容。 |
敏感性 | Hygroscopic |
外观 | 粉末 |
颜色 | slightly beige |
气味 | Odorless |
Merck | 13,7225 |
物化性质 | 本品为自猪、羊或牛的胃黏膜中提取的胃蛋白酶,为白色或淡黄色的粉末。溶于水,水溶液显酸性。等电点pH=1。难溶于乙醇、氯仿、乙醚。无毒、有肉类的特殊气味;有引湿性。干酶较稳定,加热到1000C持续10min不失活。其水溶液加热到700C以上或pH6.2以上开始失活,当pH>8时呈不可逆失活。在强酸性溶液中较稳定,最适为pH为1.5~2。其消化力以含0.2%~0.4%盐酸(pH1.6~1.8)时为最强,故常与稀盐酸合用。含糖胃蛋白酶制剂:本品系胃蛋白酶用乳糖、葡萄糖或蔗糖稀释制得的白色或淡黄色的粉末;味甜、无霉、有肉类的特殊气味;不溶于乙醇、乙醚、氯仿;有引湿性;水溶液显酸性,lg能消化凝固的卵蛋白600g。 |
MDL号 | MFCD00081840 |
危险品标志 | Xn - 有害物品 |
风险术语 | R36/37/38 - 刺激眼睛、呼吸系统和皮肤。 R42 - 吸入可能致敏。 R42/43 - 吸入及皮肤接触可能致敏。 |
安全术语 | S22 - 切勿吸入粉尘。 S24 - 避免皮肤接触。 S26 - 不慎与眼睛接触后,请立即用大量清水冲洗并征求医生意见。 S36/37 - 穿戴适当的防护服和手套。 S36 - 穿戴适当的防护服。 S45 - 若发生事故或感不适,立即就医(可能的话,出示其标签)。 |
WGK Germany | 1 |
RTECS | SC6132000 |
FLUKA BRAND F CODES | 3-10 |
TSCA | Yes |
海关编号 | 35079090 |
上游原料 | 乳糖 二醋吗啡 |
下游产品 | 胃膜素 |
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本品系自猪、羊或牛的胃黏膜中提取制得的胃蛋白酶。按干燥品计算,每lg中含胃蛋白酶活力不得少于3800单位。
本品为白色至淡黄色的粉末;无霉败臭;有引湿性;水溶液显酸性反应。
取本品的水溶液,加5%鞣酸或25%氯化钡溶液,即生成沉淀。
取本品,在100°C干燥4小时,减失重量不得过5.0%(通则0831)。
取本品,照非无菌产品微生物限度检查:微生物计数法(通则1105)和控制菌检查法(通则1106)检査。lg供试品中需氧菌总数不得过5000cfu,霉菌和酵母菌总数不得过lOOcfu,不得检出大肠埃希菌。10g供试品中不得检出沙门菌。
精密称取酪氨酸对照品适量,加盐酸溶液(取lmol /L盐酸溶液65ml,加水至1000ml)溶解并定量稀释制成每lml中含0.5mg的溶液。
取本品适量,精密称定,加上述盐酸溶液溶解并定量稀释制成每lml中约含0.2〜0.4单位的溶液。
取试管6支,其中3支各精密加人对照品溶液lml ,另3支各精密加人供试品溶液lml ,置37°C±0. 5°C水浴中,保温5分钟,精密加人预热至37°C 士0.5°C的血红蛋白试液5ml,摇匀,并准确计时,在37°C±0. 5°C水浴中反应10分钟,立即精密加入5%三氯醋酸溶液5ml,摇匀,滤过,取续滤液备用。另取试管2支,各精密加人血红蛋白试液5ml,置37°C± 0.5°C水浴中保温10分钟,再精密加人5% 三氯醋酸溶液5ml,其中1支加供试品溶液1ml,另1支加上述盐酸溶液lml,摇匀,滤过,取续滤液,分别作为供试品和对照品的空白对照,照紫外-可见分光光度法(通则0401),在275nm的波长处测定吸光度,算出平均值As和A1。
助消化药。
密封,在阴凉干燥处保存。
本品含胃蛋白酶活力不得少于120单位。
本品为糖衣片,除去包衣后显微黄色。
取本品,适量,加水研磨,使溶解,滤过,滤液照胃蛋白酶项下的鉴别试验,显相同的反应。
取本品5片,置研钵中,加胄蛋白酶效价测定项下的盐酸溶液适量,研磨均匀,全量转移至250ml量瓶中,用上述盐酸溶液稀释至刻度,摇匀,精密量取适量,用上述盐酸溶液定量稀释制成每lml中约含0.2〜0.4单位的溶液,作为供试品溶液,照胃蛋白酶项下的方法测定。
同胃蛋白酶。
120单位
密封,在凉暗处保存。
本品含胃蛋白酶活力不得少于480单位。
本品为类白色至黄色颗粒。
取本品适量,加水研磨使溶解,滤过,滤液照胃蛋白酶项下的鉴别试验,显相同的反应。
取装量差异项下的内容物,混匀,精密称取适量(约相当于胃蛋白酶240单位),用胃蛋白酶效价测定项下的盐酸溶液溶解并定量稀释制成每lml中约含0.2〜0.4单位的溶液,作为供试品溶液,照胃蛋白酶项下的方法测定。
同胃蛋白酶。
480单位
密封,在阴凉干燥处保存。
本品系胃蛋白酶用乳糖、葡萄糖或蔗糖稀释制得。按干燥品计算,每lg中含蛋白酶活力不得少于120单位或1200单位。
本品为白色至淡黄色的粉末;无霉败臭。
取本品的水溶液,照胃蛋白酶项下的鉴别试验,显相同反应。
取本品,照胃蛋白酶项下的方法测定。
同胃蛋白酶。
(l)lg:120单位(2 )lg:1200单位
密封,在阴凉干燥处保存