中文名 | 5,5'-二巯基(2-硝基苯甲酸) |
英文名 | 5,5-Dithiobis(2-nitrobenzoic acid) |
别名 | 二硫代硝基苯甲酸 双(3-羧基-4-硝基苯基)二硫 5,5'-二巯基(2-硝基苯甲酸) 5,5'二硫代双(2-硝基苯甲酸) 5,5-二硫代双(2-硝基苯甲酸) 5,5'-二硫代-双(硝基苯甲酸) 5,5'-二硫双(2-硝基苯甲酸) 5,5'-二硫代双(2-硝基苯甲酸) 5,5-二硫代双-(2-硝基苯甲酸) 5,5'-二硫代二(2-硝基苯甲酸) 5,5'-二硫代双-(2-硝基苯甲酸) 6,6ˊ-二硝基-3,3ˊ-二硫代苯甲酸 5,5’二硫代双(2-硝基苯甲酸)(DTNB) |
英文别名 | DTNB Ellmans Ellmanns Ellmans'reagent Ellmsn'sreagent ELLMANS' REAGENT ELLMAN'S REAGENT ellmann's reagent 3-Carboxy-4-nitrophenyl disulfide 3-CARBOXY-4-NITROPHENYL DISULFIDE 5,5-Dithiobis(2-nitrobenzoic acid) 3-Carboxy-4-nitrophenyl disulphide 5,5-DITHIO-BIS(2-NITROBENZOIC ACID) 5,5'-DITHIOBIS(2-NITROBENZOIC ACID) BIS(3-CARBOXY-4-NITROPHENYL) DISULFIDE 3,3'-disulfanediylbis(6-nitrobenzoate) Bis(3-carboxy-4-nitrophenyl) disulphide 3,3'-disulfanediylbis(6-nitrobenzoic acid) Bis(3-carboxy-4-nitrophenyl) disulphide~3-Carboxy-4-nitrophenyl disulphide~Ellmans |
CAS | 69-78-3 |
EINECS | 200-714-4 |
化学式 | C14H8N2O8S2 |
分子量 | 396.35 |
InChI | InChI=1/C14H8N2O8S2/c17-13(18)9-5-7(1-3-11(9)15(21)22)25-26-8-2-4-12(16(23)24)10(6-8)14(19)20/h1-6H,(H,17,18)(H,19,20)/p-2 |
InChIKey | KIUMMUBSPKGMOY-UHFFFAOYSA-N |
密度 | 1.67 |
熔点 | 240-245°C (dec.)(lit.) |
沸点 | 671.9±55.0 °C(Predicted) |
闪点 | 360.1°C |
水溶性 | Soluble in water and ethanol. |
蒸汽压 | 5.73E-19mmHg at 25°C |
溶解度 | 你有关于这个产品的溶解度信息,你想分享 |
折射率 | 1.6900 (estimate) |
酸度系数 | 1.57±0.25(Predicted) |
存储条件 | Keep in dark place,Sealed in dry,Room Temperature |
稳定性 | 稳定。与强碱、强氧化剂不相容。 |
敏感性 | Thermal sensitivity |
外观 | 黄色固体 |
颜色 | Pale Yellow to Light Yellow |
Merck | 14,3551 |
BRN | 1896821 |
MDL号 | MFCD00007140 |
体外研究 | DTNB reacts with the free sulfhydryl side chain of cysteine to form an S-S bond between the protein and a thionitrobenzoic acid (TNB) residue. The main advantage of DTNB over alternative reagents (e.g., N-ethylmaleimide or iodoacetamide) is in the selectivity of this reagent and in the ability to follow the course of the reaction spectrophotometrically. Modification of Enzyme with DTNB: Modification of the SH groups of the enzyme is carried out by reacting 10 μL of 10 mM DTNB solution (about a 20-fold molar excess) at room temperature with 0.6 mL of enzyme solution (0.807 mg/mL) in 80 mM phosphate buffer, pH 8.0, containing 1 mM EDTA, which has been dialyzed previously against the same buffer solution for 24 h. The number of SH groups is estimated from the increase of absorbance at 410 nm using a molar extinction coefficient of 13,600 M -1 cm -1 for thionitrobenzoate ions liberated. |
危险品标志 | Xi - 刺激性物品 |
风险术语 | 36/37/38 - 刺激眼睛、呼吸系统和皮肤。 |
安全术语 | S26 - 不慎与眼睛接触后,请立即用大量清水冲洗并征求医生意见。 S36 - 穿戴适当的防护服。 |
WGK Germany | 3 |
RTECS | DG9650000 |
FLUKA BRAND F CODES | 10 |
TSCA | Yes |
海关编号 | 29309070 |
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