restrictionendodeoxyribo-nucleasxbai
XbaI
CAS: 81295-42-3
Molecular Formula: C97H123N38O58P9
restrictionendodeoxyribo-nucleasxbai - Names and Identifiers
restrictionendodeoxyribo-nucleasxbai - Physico-chemical Properties
| Molecular Formula | C97H123N38O58P9 |
| Molar Mass | 3028.01 |
| Appearance | buffered aqueous glycerol solution |
| Storage Condition | −20°C |
restrictionendodeoxyribo-nucleasxbai - Risk and Safety
| WGK Germany | 1 |
| RTECS | RF0758900 |
restrictionendodeoxyribo-nucleasxbai - Introduction
XbaI (X after BamHI, A means Aerogenes) is an endonuclease that belongs to the family of restriction endonucleases. It is produced by a type of bacteria called Xanthomonas bacteria.
The enzymatic activity of XbaI depends on the temperature in the presence of magnesium ions, and the optimum temperature is 37°C. It recognizes a specific DNA sequence, the 5 '-TCTAGA-3', and divides the DNA into two pieces for two positions of that sequence. The cleaved DNA ends typically result in sticky ends that have single-stranded DNA overhangs, making the sticky ends easy to attach to other DNA molecules.
XbaI has a wide range of applications in molecular biology research. It is commonly used in DNA sequencing, subcloning, plasmid construction and other experiments. Due to its specific cleavage sequence, it can be used for the identification and localization of specific DNA fragments.
The preparation of XbaI is usually through genetic engineering methods, the corresponding DNA sequence in the Xanthomonas bacteria genome is put into a suitable expression vector, and the expression and purification are carried out by host cells such as E. coli.
In safe operation, pay attention to follow the laboratory's biosafety procedures when using XbaI. XbaI is an enzyme that is not directly toxic, but still requires careful handling and proper storage. Any enzyme manipulation should avoid direct contact with the skin and eyes, and avoid inhalation or ingestion. Appropriate personal protective equipment, such as laboratory gloves and glasses, should be worn. In addition, follow proper waste disposal procedures to ensure safe disposal of enzymes.
The enzymatic activity of XbaI depends on the temperature in the presence of magnesium ions, and the optimum temperature is 37°C. It recognizes a specific DNA sequence, the 5 '-TCTAGA-3', and divides the DNA into two pieces for two positions of that sequence. The cleaved DNA ends typically result in sticky ends that have single-stranded DNA overhangs, making the sticky ends easy to attach to other DNA molecules.
XbaI has a wide range of applications in molecular biology research. It is commonly used in DNA sequencing, subcloning, plasmid construction and other experiments. Due to its specific cleavage sequence, it can be used for the identification and localization of specific DNA fragments.
The preparation of XbaI is usually through genetic engineering methods, the corresponding DNA sequence in the Xanthomonas bacteria genome is put into a suitable expression vector, and the expression and purification are carried out by host cells such as E. coli.
In safe operation, pay attention to follow the laboratory's biosafety procedures when using XbaI. XbaI is an enzyme that is not directly toxic, but still requires careful handling and proper storage. Any enzyme manipulation should avoid direct contact with the skin and eyes, and avoid inhalation or ingestion. Appropriate personal protective equipment, such as laboratory gloves and glasses, should be worn. In addition, follow proper waste disposal procedures to ensure safe disposal of enzymes.
Last Update：2024-04-09 21:04:16
Supplier List
Spot supply
Product Name: Xba I Visit Supplier Webpage Request for quotationTel: 609-228-6898
Email: sales@medchemexpress.com
tech@medchemexpress.com
Mobile: 609-228-6898
Product Name: XbaI Visit Supplier Webpage Request for quotation
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Spot supply
Product Name: Xba I Visit Supplier Webpage Request for quotationTel: 609-228-6898
Email: sales@medchemexpress.com
tech@medchemexpress.com
Mobile: 609-228-6898
Product Name: XbaI Visit Supplier Webpage Request for quotation
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
Tel: 18301782025
Email: 3008007409@qq.com
Mobile: 18021002903
QQ: 3008007409
View History
restrictionendodeoxyribo-nucleasxbai
三异酰丙酮化钒
Kiwalon Polyester Violet 4RF
GSK-2141795,UPROSERTIB
RT 7 (coal tar)
POTASSIUM NITRITE R. G.
Silver(I) hydrogenfluoride
2-Octene, cis-
Benzeneamine-d7
三异酰丙酮化钒
Kiwalon Polyester Violet 4RF
GSK-2141795,UPROSERTIB
RT 7 (coal tar)
POTASSIUM NITRITE R. G.
Silver(I) hydrogenfluoride
2-Octene, cis-
Benzeneamine-d7