deoxyribonucleicac.
PUC18
CAS: 9007-49-2
Molecular Formula: C15H31N3O13P2
deoxyribonucleicac. - Names and Identifiers
deoxyribonucleicac. - Physico-chemical Properties
| Molecular Formula | C15H31N3O13P2 |
| Molar Mass | 523.366462 |
| Density | 1.00g/mLat 20°C |
| Water Solubility | Dissolve in TE buffer or water at 2mg/ml |
| Solubility | 50 mM Tris pH 8.0: 10mg/mL, clear, faintly brown-yellow |
| Appearance | Liquid |
| Color | White to Light yellow to Light orange |
| Storage Condition | -20°C |
deoxyribonucleicac. - Risk and Safety
| Safety Description | S22 - Do not breathe dust. S24/25 - Avoid contact with skin and eyes. |
| WGK Germany | 3 |
| RTECS | HG1933000 |
| FLUKA BRAND F CODES | 3-10-21 |
| TSCA | Yes |
| HS Code | 29349990 |
deoxyribonucleicac. - Introduction
PUC18 is a commonly used plasmid consisting of an archaeal polymerase gene promoter and a circular DNA segment encoding amino acids and resistance genes.
PUC18 is widely used in molecular biology experiments. As a plasmid vector, it can be used for cloning, expression and amplification of recombinant DNA. PUC18 has resistance genes, usually including antibiotic resistance genes, which can be screened against transformed or transcribed bacteria by antibiotic screening methods.
The method of preparing PUC18 is usually by transforming the PUC18DNA into host cells such as E. coli, and then using technical means such as antibiotic screening to select bacteria containing PUC18.
Regarding the safety information of PUC18, the following points need to be paid attention. First of all, the plasmid transformation process may have a certain impact on the growth and metabolism of host bacteria; secondly, the plasmid may carry antibiotic resistance genes, which may have a certain impact on the treatment of antibiotics; in addition, the plasmid transformation process Need to pay attention to biosafety operations to avoid possible harm to the human body or the environment.
PUC18 is widely used in molecular biology experiments. As a plasmid vector, it can be used for cloning, expression and amplification of recombinant DNA. PUC18 has resistance genes, usually including antibiotic resistance genes, which can be screened against transformed or transcribed bacteria by antibiotic screening methods.
The method of preparing PUC18 is usually by transforming the PUC18DNA into host cells such as E. coli, and then using technical means such as antibiotic screening to select bacteria containing PUC18.
Regarding the safety information of PUC18, the following points need to be paid attention. First of all, the plasmid transformation process may have a certain impact on the growth and metabolism of host bacteria; secondly, the plasmid may carry antibiotic resistance genes, which may have a certain impact on the treatment of antibiotics; in addition, the plasmid transformation process Need to pay attention to biosafety operations to avoid possible harm to the human body or the environment.
Last Update：2024-04-09 19:06:00
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