| Overview | serine derivatives, such as optically active amino acids at the alpha position (β-hydroxy-α-L-amino acids, Β-hydroxy-α-D-amino acid) is expected to be used as an intermediate for drugs. Therefore, the development of Serine Derivatives in industry has been promoted. As a method for producing a serine derivative using an enzyme, for example, a serine derivative such as 2-methyl-DL-serine hydrate can be produced using microorganisms of the genera Xanthomonas, Ralstonia and Variovorax. |
| preparation | synthesis of 2-methyl-DL-serine hydrate: JM109/pSFNRHMT-Y339S strain was cultured overnight in LB agar medium containing mg/l ampicillin, this was then inoculated into 50ml of LB medium containing 120 mg/l ampicillin in a ML Sakaguchi flask and subjected to reciprocations/and incubation for 17 hours at 37°C. The obtained cells were collected by centrifugation (8,000 x g,10 minutes) and then washed with 30ml of 50mm potassium phosphate buffer (pH 7.4) containing 0.1mm pyridoxal phosphate. Then, a cell suspension was prepared using 50ml of the same buffer. Cells collected from 2ml cell suspension by centrifugation (18,000 x g,10 min) were added to 10mm formaldehyde, 30mm L-alanine, 0.1mm pyridoxal phosphate and 50mm potassium phosphate buffer (to which 100 μl of a solution consisting of pH 7.4 was added, and reacted at 30 ° C. For 1 hour. After completion of the reaction, the supernatant obtained by centrifugation (18,000 ×g,5 minutes, 4 ° C.) was subjected to ESI-MS analysis, and a molecular ion peak of 2-methyl-dl-serine hydrate was observed. |
