9001-37-0
glucose oxidase from aspergillus niger
CAS: 9001-37-0
Molecular Formula:
9001-37-0 - Names and Identifiers
| Name | glucose oxidase from aspergillus niger |
| Synonyms | notatin 1.1.3.4 oxidaseglucose glucose oxidase from aspergillus niger |
| CAS | 9001-37-0 |
| EINECS | 232-601-0 |
9001-37-0 - Physico-chemical Properties
| Density | 1.00g/mLat 20°C |
| Solubility | Soluble in water, completely insoluble in ether, chloroform, butanol, pyridine, glycerin, ethylene glycol and other organic solvents, 50% acetone, 66% methanol can make it precipitate. |
| Vapor Presure | 0.004Pa at 25℃ |
| Appearance | Yellow powder |
| Color | yellow |
| Merck | 14,4460 |
| PH | 4.5 |
| Storage Condition | -20°C |
| Stability | Stable. Incompatible with strong oxidizing agents. Protect from moisture. |
| MDL | MFCD00131182 |
| Physical and Chemical Properties | Near white powder to brown liquid. Soluble in water, almost insoluble in ethanol, chloroform and ether. |
| Use | This product is for scientific research only and shall not be used for other purposes. |
9001-37-0 - Risk and Safety
| Risk Codes | R42 - May cause sensitization by inhalation R36/37/38 - Irritating to eyes, respiratory system and skin. |
| Safety Description | S45 - In case of accident or if you feel unwell, seek medical advice immediately (show the label whenever possible.) S22 - Do not breathe dust. S23 - Do not breathe vapour. S36 - Wear suitable protective clothing. S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S24/25 - Avoid contact with skin and eyes. |
| WGK Germany | 1 |
| RTECS | RQ8452000 |
| FLUKA BRAND F CODES | 3-10 |
| HS Code | 35079090 |
9001-37-0 - Nature
Open Data Verified Data
light yellow or gray-yellow powder, soluble in water into yellow-green solution, insoluble in ether, chloroform, butanol, pyridine, glycerol, ethylene glycol, dioxane, 1,3 propylene glycol and formamide. It can be precipitated by 50% acetone or 66% methanol. Molecular weight of glucose oxidase from mold
About 150000, isoelectric point pH 4.2~4.3, the optimum pH 5.5~5.8, the optimum temperature 30~35 ℃, acid, alkali and high temperature can damage.
Last Update：2024-01-02 23:10:35
9001-37-0 - Use
Open Data Verified Data
commonly used diagnostic enzymes. Analysis of glucose. Removal of excess glucose or oxygen is a stabilizer for preparations of vitamin C and vitamin B12. Preparation of urine sugar and blood glucose test paper. Desaccharification of protein, storage of canned liquor, pharmaceutical industry, etc.
Last Update：2022-01-01 11:16:47
9001-37-0 - Safety
Open Data Verified Data
5G packaging, sealed and dried, stored below 4 ℃, the solution should prevent freezing.
Last Update：2022-01-01 11:16:47
9001-37-0 - Vitality definition
One unite will oxidize one micromole of glucose per min at pH5.7 at 37℃
Last Update：2023-08-16 21:32:38
9001-37-0 - Molecular weight
approx 150 kDa (SDS-PAGE).
Last Update：2023-08-16 21:32:38
9001-37-0 - Isoelectric point
4.2.
Last Update：2023-08-16 21:32:38
9001-37-0 - Reference Information
| LogP | -1.3 at 20℃ |
| EPA chemical substance information | information provided by: ofmpeb.epa.gov (external link) |
| Introduction | glucose oxidase is an enzyme found in molds such as Penicillium notatum and honey. It catalyzes the reaction of D-glucose O2D-gluconic acid (δ-lactone) H2O2. EC 1.1.3.4. The enzyme of Penicillium sp. Was noted for its outstanding antibacterial properties. Therefore, there is also the name of glucose oxidase (notatin), and it is now clear that the antibacterial property is due to the sterilization characteristics of H2O2 generated by the reaction. The purified product contains two molecules of FAD, and can be reacted with 2,6, dichlorophenol and indophenol in addition to O2 as an electron acceptor. The enzyme is specific for glucose. |
| Use | Antioxidant, color fixative, preservative, enzyme preparation. Flour gluten enhancer. Enhance the strength of the gluten. To improve the extensibility of dough, increase the volume of bread. The use of glucose oxidase can remove oxygen from food and containers, thereby effectively preventing food from deteriorating, and thus can be applied to the packaging of tea, ice cream, milk powder, beer, fruit wine and other beverage products. It is a green biological food insurance agent obtained by microbial fermentation and the most advanced purification technology. It is non-toxic and has no side effects. Can remove dissolved oxygen in food, play a fresh, protect color, anti-Browning, protect Vitamin C, extend the role of food quality reporting period. enzyme preparation. Mainly used for milk, cheese, eggs, drinks and salad. commonly used diagnostic enzymes. Determination of glucose in plasma. enzyme preparation. It is mainly used to remove glucose from egg liquid to prevent discoloration and deterioration of finished protein products during storage. The highest dosage of 500 mg/kg. Deoxygenation of citrus beverages and beer. To prevent color darkening, reduce flavor and metal dissolution. The highest dosage was 10 mg/kg. For whole milk powder, grains, cocoa, coffee, shrimp, meat and other food. Browning caused by glucose can be prevented. The H2O2 generated by the oxidation of glucose can oxidize the-SH group in gluten to S-S-group, which helps to form a better protein network structure between gluten proteins. Therefore, in recent years for the manufacture of bread, the effect is good, can be used to replace the carcinogenic potassium bromate, by more and more eyes. glucose oxidase can be used to remove glucose from protein, prevent discoloration and deterioration of protein products during storage, and the maximum usage amount is 500mg/kg, the maximum amount of metal elution was 10mg/kg. It is used for biochemical research, clinically used as a diagnostic enzyme, used for glucose analysis, preparation of urine glucose and blood glucose test paper, and used as a stabilizer for vitamin C and B12 preparations in the pharmaceutical industry, can also be used for the removal of sugar protein, canned wine storage. commonly used diagnostic enzymes. Analysis of glucose. Excess glucose or oxygen is removed and is a stabilizer for the vitamin C and B12 formulations. Preparation of urine sugar and blood glucose test paper. Glucose oxidation reagent for the determination of blood glucose by oxidase method. macroporous adsorption resin is a kind of organic polymer which is insoluble in acid, alkali and various organic solvents, application of macroporous adsorption resin separation technology is the end of the 20th century, 60 years after the development of ion exchange resin separation one of the new technology |
| Application | as a food additive, glucose oxidase has the function of converting glucose and removing residual oxygen-maintaining the color, flavor, the function of taste, stable product quality, prolonged storage time. (1) the glucose oxidase system can oxidize the sulfhydryl group (-SH) in the gluten molecule to disulfide bond (-S-S-), so as to enhance the strength of gluten, improve the extensibility of dough, increase the volume of bread, can replace the human body has carcinogenic characteristics of potassium bromate (KbrO4). In the production of noodles, glucose oxidase helps to form a better protein network structure between gluten proteins and increases the bite strength of noodles. (2) the refined enzyme can be used for clinical diagnosis, rapid and accurate determination of glucose content in body fluids, and provide reliable data for doctors to accurately judge the patient's condition. (3) crude enzyme preparation can be used |
| toxicity | ADI is not specified (FA0/wH0,2001). for those obtained from Aspergillus niger, ADI does not make special provisions; For those obtained from Penicillium, no provision has been made (FAO/WHO,2001). |
| usage limit | GB 2760-2000: beer craft 80ml/L; Wheat flour, 780GODF. |
| production method | the culture medium was prepared by deep ventilation culture method with Aspergillus niger and superoides as production bacteria, the product was then precipitated by dialysis, column chromatography, and ammonium sulfate fractionation. A significant amount of extracellular glucose oxidase can be produced by deep culture with Penicillium No.778 using spent molasses, KH2PO4, NaNO3 or the like as a medium. The Aspergillus niger var. Was used in the United States, the Penicillium sp. Was used in Japan, and the Penicillium sp. Was used in the former Soviet Union. Penicillium aureus (P.chrysogenum, Penicillium sp. notatum) under controlled conditions for submerged fermentation, with ethanol, acetone to make the precipitation of kaolin or aluminum hydroxide after adsorption and ammonium sulfate salting out, refined. obtained by controlled culture of Aspergillus niger var. |
Last Update：2024-04-09 21:01:54
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