54375-47-2
4-methylcoumarin-6-ylmethyliminodiacetic acid
CAS: 54375-47-2
Molecular Formula: C15H15NO7
54375-47-2 - Names and Identifiers
| Name | 4-methylcoumarin-6-ylmethyliminodiacetic acid |
| Synonyms | CALCEIN BLUE TIMTEC-BB SBB002950 UMBELLIFERONE COMPLEXONE 4-methylcoumarin-6-ylmethyliminodiacetic acid 4-METHYLUMBELLIFERONE-8-METHYLIMINODIACETIC ACID B-METHYLUMBELLIFERONE METHYLENEIMINODIACETIC ACID 4-METHYLUMBELLIFERONE METHYLENEIMINODIACETIC ACID 4-METHYLUMBELLIFERONE-6-METHYLENEIMINODIACETIC ACID 4-METHYLUMBELLIFERONE-8-METHYLENEIMINODIACETIC ACID |
| CAS | 54375-47-2 |
| EINECS | 259-129-8 |
| InChI | InChI=1/C15H15NO7/c1-8-4-14(22)23-15-9(8)2-3-11(17)10(15)5-16(6-12(18)19)7-13(20)21/h2-4,17H,5-7H2,1H3,(H,18,19)(H,20,21)/p-1 |
54375-47-2 - Physico-chemical Properties
| Molecular Formula | C15H15NO7 |
| Molar Mass | 321.28 |
| Density | 1.512±0.06 g/cm3(Predicted) |
| Melting Point | >200℃ |
| Boling Point | 636.2±55.0 °C(Predicted) |
| Flash Point | 338.6°C |
| Solubility | Soluble in water,methanol, dimethyl sulfoxide |
| Vapor Presure | 4.67E-17mmHg at 25°C |
| Appearance | powder |
| Color | White to pale yellow |
| Maximum wavelength(λmax) | 360 nm |
| pKa | 1.73±0.10(Predicted) |
| Storage Condition | 2-8℃ |
| MDL | MFCD00006867 |
| In vitro study | The fluorescence of Calcein Blue (CB) is quenched by the Fe 2+ ion in the Calcein Blue-Fe 2+ complex. When dopamine is added to a solution of the Calcein Blue-Fe 2+ complex, a dopamine-Fe 2+ complex is formed as the result of a ligand exchange reaction between Calcein Blue and dopamine which permits the fluorescence from Calcein Blue to be recovered. The fluorescence intensity at the wavelength of 440 nm (at the excitation wavelength of 340 nm) is found to be proportional to the concentration of the dopamine added to the Calcein Blue-Fe 2+ complex solution, which permits dopamine to be quantitatively determined. The Calcein Blue-Fe 2+ complex shows a high selectivity for dopamine among the catecholamines and related compounds examined. |
54375-47-2 - Risk and Safety
| Hazard Symbols | Xi - Irritant |
| Risk Codes | 36/37/38 - Irritating to eyes, respiratory system and skin. |
| Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36 - Wear suitable protective clothing. |
| WGK Germany | 3 |
54375-47-2 - Preparation solution concentration reference
| 1mg | 5mg | 10mg | |
|---|---|---|---|
| 1 mM | 3.113 ml | 15.563 ml | 31.125 ml |
| 5 mM | 0.623 ml | 3.113 ml | 6.225 ml |
| 10 mM | 0.311 ml | 1.556 ml | 3.113 ml |
| 5 mM | 0.062 ml | 0.311 ml | 0.623 ml |
Last Update：2024-01-02 23:10:35
54375-47-2 - Reference Information
| overview | the chemical name of calcein blue (CaleCinblue) is 4-methyl umbelliferone -6-methyl iminodiacetic acid, which is a newly developed reagent applied abroad in recent years. at present, it is only seen in the reagent catalogues of a few manufacturers in the United States, Switzerland and West Germany. Shanghai Chemical Reagent Procurement and Supply Station should be required by the relevant domestic units to introduce the trial. Calcein blue is a colorless crystalline powder, easily soluble in alkaline solution, and produces long-lasting blue fluorescence, slightly soluble in water, ethanol and acetone. |
| use | calcein blue can be used as a metal fluorescent indicator, calcium can be titrated by EDTA complexation at high pH, copper, nickel and diamond can be titrated by complexation at low pH, and can also be used for the study of t-RNA binding to heavy metals and as a fluorescent marker of bone. |
| biological activity | Calcein Blue is an impermeable membrane fluorescent dye, which is a coumarin derivative containing iminodiacetic acid structure. Calcein Blue is also a metal fluorescent discoloration indicator. |
| in vitro study | The fluorescence of Calcein Blue (CB) is quenched by the Fe 2 ion in the Calcein Blue-Fe 2 complex. When dopamine is added to a solution of the Calcein Blue-Fe 2 complex, A dopamine-Fe 2 complex is formed as the result of a ligand exchange reaction between Calcein Blue and dopamine which permits the fluorescence from Calcein Blue to be recovered. The fluorescence intensity at the wavelength of 440 nm (at the excitation wavelength of 340 nm) is found to be proportional to the concentration of the dopamine added to the Calcein Blue-Fe 2 complex solution, which permits dopamine to be quantitatively determined. The Calcein Blue-Fe 2 complex shows a high selectivity for dopamine among the catecholamines and related compounds examined. |
| biological field application | Copper indicator; calcium indicator; cadmium indicator; iron (II) indicator; iron (III) indicator; fluoride indicator; lanthanides indicator; magnesium indicator; silver indicator; sulfate indicator; zinc indicator; treating ath |
Last Update：2024-04-09 15:16:38
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