3x DYKDDDDK Tag MedChemExpress (MCE)

Product Name	L-Lysine, L-α-aspartyl-L-tyrosyl-L-lysyl-L-α-aspartyl-L-α-aspartyl-L-α-aspartyl-L-α-aspartyl-L-lysyl-L-α-aspartyl-L-tyrosyl-L-lysyl-L-α-aspartyl-L-α-aspartyl-L-α-aspartyl-L-α-aspartyl-L-lysyl-L-α-aspartyl-L-tyrosyl-L-lysyl-L-α-aspartyl-L-α-aspartyl-L-α-aspartyl-L-α-aspartyl-
Synonyms	3x DYKDDDDK Tag L-Lysine, L-α-aspartyl-L-tyrosyl-L-lysyl-L-α-aspartyl-L-α-aspartyl-L-α-aspartyl-L-α-aspartyl-L-lysyl-L-α-aspartyl-L-tyrosyl-L-lysyl-L-α-aspartyl-L-α-aspartyl-L-α-aspartyl-L-α-aspartyl-L-lysyl-L-α-aspartyl-L-tyrosyl-L-lysyl-L-α-aspartyl-L-α-aspartyl-L-α-aspartyl-L-α-aspartyl-
CAS	921765-76-6
EINECS
Chemical Formula	C123H176N30O58
Molecular Weight	3002.91
inchi
Package	1 mg;5 mg;10 mg
Price	Email to quote
Descriptions	3x DYKDDDDK Tag 3x DYKDDDDK Tag MedChemExpress (MCE) HY-P3332 921765-76-6 96.34% Sealed storage, away from moisture Powder -80°C 2 years -20°C 1 year In solvent : -80°C, 6 months Descriptions 3x DYKDDDDK Tag 3x DYKDDDDK Tag MedChemExpress (MCE) HY-P3332 921765-76-6 96.34% Sealed storage, away from moisture Powder -80°C 2 years -20°C 1 year In solvent : -80°C, 6 months -20°C, 1 month (sealed storage, away from moisture) Room temperature in continental US may vary elsewhere. 3x DYKDDDDK Tag is a useful tool for investigating the function and localization of proteins whose antibodies (Abs) are not available. Often it is also used in a 3X FLAG format (3x DYKDDDDK Tag) for purifying difficult proteins that accumulate in low abundance . HEK293 cells transiently transfected with HARS-expressing plasmids were harvested and lysed in CelLytic M buffer containing mammalian protease inhibitor cocktail for 20 min at 4 °C. FLAG-tagged HARS is purified by binding to an anti-DYDDDDK resin per manufacturer protocol and eluted by competition with 3X-DYKDDDDK peptide in buffer containing 50 mM Tris-HCl pH 7.4, and 150 mM NaCl (purification of human HARS)[2]. Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys-Asp-Tyr-Lys-Asp-Asp-Asp-Asp-Lys \| \| \| \| \| \| \| \| \| \| [1]. Mishra V. Affinity Tags for Protein Purification. Curr Protein Pept Sci. 2020 21(8):821-830. [Content Brief] [2]. Abbott JA, et al. The Usher Syndrome Type IIIB Histidyl-tRNA Synthetase Mutation Confers Temperature Sensitivity. Biochemistry. 2017 56(28):3619-3631. [Content Brief]
Supplier Website	https://www.medchemexpress.com/3x-dykddddk-tag.html
Last Update	2025-05-21 16:50:25

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