6-FAM MedChemExpress (MCE)

Product Name	6-carboxyfluorescein
Synonyms	6-FAM, 6-CF Carboxyfluorescein 6-carboxyfluorescein 6-Carboxyfluorescein Hydrate 6-Carboxyfluorescein (6-FAM) 6-Carboxyfluorescein,6-FAM, FLUOS 3',6'-dihydroxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-carboxylic acid Synonyms 6-FAM, 6-CF Carboxyfluorescein 6-carboxyfluorescein 6-Carboxyfluorescein Hydrate 6-Carboxyfluorescein (6-FAM) 6-Carboxyfluorescein,6-FAM, FLUOS 3',6'-dihydroxy-1-oxospiro[2-benzofuran-3,9'-xanthene]-5-carboxylic acid 3',6'-dihydroxy-1-oxo-spiro[2-benzofuran-3,9'-xanthene]-5-carboxylic acid 3',6'-dihydroxy-1-keto-spiro[isobenzofuran-3,9'-xanthene]-5-carboxylic acid 3',6'-dihydroxy-3-oxo-3H-spiro[2-benzofuran-1,9'-xanthene]-6-carboxylic acid 3-Oxo-3',6'-dihydroxy-1,3-dihydrospiro[isobenzofuran-1,9'-[9H]xanthene]-6-carboxylic acid
CAS	3301-79-9
EINECS	636-503-2
Chemical Formula	C21H12O7
Molecular Weight	376.32
inchi	InChI=1/C21H12O7/c22-11-2-5-14-17(8-11)27-18-9-12(23)3-6-15(18)21(14)16-7-10(19(24)25)1-4-13(16)20(26)28-21/h1-9,22-23H,(H,24,25)
Package	25 mg;100 mg;500 mg
Price	Email to quote
Descriptions	6-FAM 6-FAM MedChemExpress (MCE) HY-66021 3301-79-9 6-Carboxyfluorescein 99.88% -20°C, protect from light, stored under nitrogen In solvent : -80°C, 6 months Descriptions 6-FAM 6-FAM MedChemExpress (MCE) HY-66021 3301-79-9 6-Carboxyfluorescein 99.88% -20°C, protect from light, stored under nitrogen In solvent : -80°C, 6 months -20°C, 1 month (protect from light, stored under nitrogen) Room temperature in continental US may vary elsewhere. 6-FAM (6-Carboxyfluorescein) is an isomer of carboxyfluorescein and is mainly used for sequencing and labeling of nucleic acids. In cell experiments, we use 6-Carboxyfluorescein (6-FAM) labeled oligonucleotide probes, mainly through fluorescence in situ hybridization (FISH) technology[1] (1) Sample preparation: Prepare chromosomal samples from chicken embryo cells, including steps like colchicine treatment, hypotonic swelling, and methanol-acetic acid fixation. (2) Pre-treatment: The chromosomes are treated with RNase A, pepsin, and formaldehyde. (3) Hybridization: The 6-FAM labeled oligonucleotide is dissolved in a hybridization buffer containing E. coli tRNA (50% formamide, 2×SSC, 10% dextran sulfate) at a concentration of 20 ng/μl. After DNA and probes are denatured together, hybridization is done overnight at room temperature in a humidity chamber. (4) Washing and counterstaining: The slides post-hybridization are washed three times in 2×SSC, then dehydrated through a series of 70%-96% ethanol, air-dried, and mounted with an anti-fade reagent containing DAPI. (5) Observation and analysis: Use a Leica DM4000B fluorescence microscope equipped with a monochrome CCD camera DFC350FX and appropriate filters to observe the samples, and capture and process the dye images using QFISH software. 523 489 \| \| \| \| \| \| \| \| \| \| [1]. Alexander Stepakov, et al. Modified Synthesis of 6-carboxyfluorescein (6-FAM): Application to Probe Labeling for Conventional Cytogenetics. British Journal of Applied Science & Technology. 7(4): 423-428, 2015, Article no.BJAST.2015.160.
Supplier Website	https://www.medchemexpress.com/6-FAM.html
Last Update	2025-05-21 16:50:25

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