HOE-S 785026 MedChemExpress (MCE)

Product Name	HOE-S 785026
Synonyms	HOE-S 785026;Meta-Hoechst;Phenol, 3-[5-(4-methyl-1-piperazinyl)[2,5'-bi-1H-benzimidazol]-2'-yl]- (9CI)
CAS	132869-83-1
EINECS
Chemical Formula	C25H24N6O
Molecular Weight	424.5
inchi
Package	1 mg;5 mg;10 mg;25 mg
Price	Email to quote
Descriptions	HOE-S 785026 HOE-S 785026 MedChemExpress (MCE) HY-15561 132869-83-1 meta-Hoechst 98.76% 4°C, protect from light In solvent : -80°C, 6 months Descriptions HOE-S 785026 HOE-S 785026 MedChemExpress (MCE) HY-15561 132869-83-1 meta-Hoechst 98.76% 4°C, protect from light In solvent : -80°C, 6 months -20°C, 1 month (protect from light) Room temperature in continental US may vary elsewhere. HOE-S 785026 is a marker dye in Hoechst series. Hoechst is A live nuclear marker dye. Hoechst binds to the grooves in the DNA double strand, which tends to be A/ T-rich DNA strand. Although it binds to all nucleic acids, the A/ T-rich double strand DNA significantly enhances fluorescence intensity Therefore,Hoechst dye can be used for living cell labeling. The fluorescence intensity of Hoechst dye increases with the increase of pH of solution. General Protocol Preparation of Hoechst working solution 1.1 Preparation of the stock solution Dissolve 10 mg of in 5 mL DMSO Note: It is recommended to store the stock solution at 4℃ or -20℃ away from light and avoid repetitive freeze-thaw cycles. 1.2 Preparation of Hoechst working solution Dilute the stock solution in serum-free cell culture medium or PBS to obtain final concentration 10 μg/mL Hoechst working solution. Note: Please adjust the concentration of Hoechst working solution according to the actual situation. 1.Cell staining 2.1 Suspension cells（6-well plate） a. Centrifuge at 1000 g at 4℃ for 3-5 minutes and then discard the supernatant. Wash twice with PBS, 5 minutes each time. The cell density is 1×106/mL. b. Add 1 mL of working solution, and then incubate at room temperature for 3-10 minutes. c. Centrifuge at 400 g at 4℃ for 3-4 minutes and then discard the supernatant. d. Wash twice with PBS, 5 minutes each time. e. Resuspend cells with serum-free cell culture medium or PBS. Observation by fluorescence microscopy or flow cytometry. 2.2 Adherent cells a. Culture adherent cells on sterile coverslips. b. Remove the coverslip from the medium and aspirate excess medium. c. Add 100 μL of working solution, gently shake it to completely cover the cells,and then incubate at room temperature for 3-10 minutes. d. Wash twice with medium, 5 minutes each time.Observation by fluorescence microscopy or flow cytometry. Precautions 1. Please adjust the concentration of Hoechst working solution according to the actual situation. 2. This product is for R&D use only, not for drug, household, or other uses. 3. For your safety and health, please wear a lab coat and disposable gloves to operate. 451 356 \| \| \| \| \| \| \| \| \| \|
Supplier Website	https://www.medchemexpress.com/HOE-S-785026.html
Last Update	2025-05-21 16:50:25

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