BE-34776 MedChemExpress (MCE)

Product Name	MS-444
Synonyms	MS-444;BE-34776;5,8-dihydroxy-3-methyl-4-(9H)-naphtho(2,3-c)furanone;Naphtho[2,3-c]furan-4(9H)-one, 5,8-dihydroxy-3-methyl-
CAS	150045-18-4
EINECS
Chemical Formula	C13H10O4
Molecular Weight	230.22
inchi
Package	10 mM * 1 mL;1 mg
Price	Email to quote
Descriptions	MS-444 MS-444 MedChemExpress (MCE) HY-100685 150045-18-4 BE-34776 99.45% -20°C, protect from light, stored under nitrogen In solvent : -80°C, 6 months Descriptions MS-444 MS-444 MedChemExpress (MCE) HY-100685 150045-18-4 BE-34776 99.45% -20°C, protect from light, stored under nitrogen In solvent : -80°C, 6 months -20°C, 1 month (protect from light, stored under nitrogen) Room temperature in continental US may vary elsewhere. MS-444 inhibits the activity of purified smooth muscle myosin light chain kinase (MLCK) with an IC50 value of 10 μM. MS-444 is a small molecule RNA-binding protein HuR (ELAVL1) inhibitor. Colorectal cancer (CRC) cells that display HuR overexpression are treated with MS-444 (1-100 μM) for 48 hr with IC50s of 10.98±1.76 μM, 12.84±2.10 μM, 5.60±0.90 μM, 14.21±2.11 μM, and 10.98±1.24 μM for HCT116, HCA-7, RKO, HT-29, and SW480 cells, respectively. Growth inhibition is observed in all CRC lines with IC50 values ranging from 5.60 μM to 14.21 μM with observable effects seen at 10 μM MS-444. Contrasting effects are observed using non-transformed small intestinal (RIE-1 (IC50=40.70±3.53 μM)) and colonic (YAMC (IC50=28.16±3.23 μM)) epithelial cells. Both cell types display properties of normal intestinal epithelial cells and are proficient in 3′UTR AU-rich elements (ARE)-mRNA decay. Both non-transformed cell lines are ~3- to 4-fold less responsive to MS-444-mediated growth inhibition, with IC50 values of 40.70 μM and 28.16 μM (P[2]. To test the effects of MS-444 on CRC cell growth in vivo, mice bearing HCT116 cell xenografts receive IP injections of MS-444 (25 mg/kg bw) or vehicle every 48 hr. Over the experiment course, mice do not display any adverse effects and maintained similar weights. Anti-tumor effects of MS-444 are observed with approximately 1.7-fold reduction in tumor size. Mice treated with MS-444 show a marked 2- to 3-fold decrease in microvessel density (MVD), indicating the anti-angiogenic potential of MS-444[2]. Mice[2] Athymic nude (Nu/Nu) mice are used. HCT116 (2×106 cells) and HCA-7 (2.5×106) cells resuspended in PBS are injected into the dorsal subcutaneous tissue. Mice (n=5 per group) receive intraperitoneal (IP) injections of MS-444 (25 mg/kg) dissolved in PBS/5% N-Methyl Pyrrolidine (NMP) or vehicle control every 48 hr. Tumor growth is assayed[2]. Human colorectal cancer cell lines RKO, HCA-7, HCT116, HT-29, SW480 and the non-transformed intestinal epithelial cell lines RIE-1, YAMC are treated with varying concentrations of MS-444 (1-100 μM) for 48 hr. Cell survival is measured by MTT assay after incubation of cells for 48 hr with MS-444. Relative cell survival is calculated as percentage normalized to DMSO vehicle-treated cells and plotted to determine IC50[2]. IC50: 10 μM (myosin)[1]. Cellular Effect Cell Line Type Value Description References \| \| \| \| \| \| \| \| \| \| [1]. Satoshi Nakanishi. et al. MS-444, a new inhibitor of myosin light chain kinase from Micromonosporasp.KY7123. The Journal Of Antibiotics. 1995,48(9):948-951. [Content Brief] [2]. Fernando F. Blanco.et al, Impact of HuR inhibition by the small molecule MS-444 on colorectal cancer cell tumorigenesis. Oncotarget. 2016 Nov 8 7(45): 74043-74058. [Content Brief]
Supplier Website	https://www.medchemexpress.com/MS-444.html
Last Update	2025-04-01 14:45:47

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