sodium 4-amino-5-hydroxy-3-(4-nitrophenylazo)-6-(phenylazo)naphthalene-2,7-disulphonate
Acid Black 1
CAS: 1064-48-8
Molecular Formula: C22H17N6NaO9S2
sodium 4-amino-5-hydroxy-3-(4-nitrophenylazo)-6-(phenylazo)naphthalene-2,7-disulphonate - Names and Identifiers
sodium 4-amino-5-hydroxy-3-(4-nitrophenylazo)-6-(phenylazo)naphthalene-2,7-disulphonate - Physico-chemical Properties
| Molecular Formula | C22H17N6NaO9S2 |
| Molar Mass | 596.52 |
| Density | 1.05g/mLat 20°C |
| Melting Point | >350℃ |
| Flash Point | 30°C |
| Water Solubility | 10 g/L (25 ºC) |
| Solubility | 10g/l |
| Vapor Presure | 0Pa at 25℃ |
| Appearance | Blue-black or dark brown powder |
| Color | Dark Brown |
| BRN | 5374263 |
| PH | 9.0 (10g/l, H2O, 25℃) |
| Storage Condition | Store at RT. |
| Stability | Stable. Incompatible with strong oxidizing agents. |
| Sensitive | Hygroscopic |
| MDL | MFCD00004017 |
| Physical and Chemical Properties | Dark brown powder. Soluble in water, the aqueous solution is blue-black, and the addition of concentrated hydrochloric acid produces a green blue precipitate; The addition of sodium hydroxide solution produces a blue precipitate. Soluble in ethanol, blue, slightly soluble in acetone, insoluble in other organic solvents. It is blue green in concentrated sulfuric acid, and produces dark green blue precipitation after dilution; It is dark green solution in concentrated nitric acid and then turns into paste red; It produces blue precipitation in 10% sodium hydroxide solution. When dyeing, the color of copper ion changed slightly; The color of iron ion was slightly light green. |
| Use | Electrophoretic reagent for staining of proteins on polyacrylamide gels, agarose gels and nitrocellulose membranes. After electrophoresis, it is recommended to fix the protein on the gel. The glue is dyed with 7% (v/v) acetic acid solution of 0.1% amino black 10B for at least 2 hours, and then decolorized with 7% (v/v) acetic acid solution. The detection sensitivity is about the 20% of Coomassie Brilliant Blue R250. Redox indicator. |
sodium 4-amino-5-hydroxy-3-(4-nitrophenylazo)-6-(phenylazo)naphthalene-2,7-disulphonate - Risk and Safety
| Risk Codes | R36/37/38 - Irritating to eyes, respiratory system and skin. R10 - Flammable R34 - Causes burns R11 - Highly Flammable R67 - Vapors may cause drowsiness and dizziness R36/38 - Irritating to eyes and skin. |
| Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36 - Wear suitable protective clothing. S37/39 - Wear suitable gloves and eye/face protection S45 - In case of accident or if you feel unwell, seek medical advice immediately (show the label whenever possible.) S36/37/39 - Wear suitable protective clothing, gloves and eye/face protection. S23 - Do not breathe vapour. S16 - Keep away from sources of ignition. |
| UN IDs | UN 2924 3/PG 3 |
| WGK Germany | 3 |
| RTECS | QJ6196000 |
| TSCA | Yes |
| HS Code | 32041200 |
| Toxicity | LD50 orally in Rabbit: > 5000 mg/kg |
sodium 4-amino-5-hydroxy-3-(4-nitrophenylazo)-6-(phenylazo)naphthalene-2,7-disulphonate - Reference
| Reference Show more | 1. Zhang Danqing, Ding Xiuyun, Li Wan, Huo Guicheng. In vitro degradation of mucin by two Lactobacillus acidophilus strains [J]. Food Industry Science and Technology 2016 37(08):155-157. 2. Sun Lijie, Liu Dongxu, Li Jianming, et al. Preliminary Evaluation of Immune Effect of Two Mink Aleutian Whole Gene Recombinant Nucleic Acid Vaccines [J]. Chinese Journal of Veterinary Drugs 2017 051(012):1-6. |
sodium 4-amino-5-hydroxy-3-(4-nitrophenylazo)-6-(phenylazo)naphthalene-2,7-disulphonate - Introduction
(For reference only) Soluble in water (blue-black), ethanol (dark blue) and cellosolve (diethanol ether), slightly soluble in acetone, insoluble in other organic solvents. In case of concentrated sulfuric acid, it appears blue-green, diluted to dark green and blue precipitate. In case of concentrated nitric acid, it shows a dark green solution. Blue solution with 10% sodium hydroxide solution showing red fluorescence. The aqueous solution is added with concentrated hydrochloric acid to form a green-blue precipitate, and a concentrated sodium hydroxide solution is added to form a blue precipitate. When dyeing, it meets copper; iron ions, the former has slightly changed in color, and the latter is slightly light green.
Last Update：2022-10-16 17:11:48
sodium 4-amino-5-hydroxy-3-(4-nitrophenylazo)-6-(phenylazo)naphthalene-2,7-disulphonate - Reference Information
| color index | 20470 |
| LogP | -1.706 at 20℃ |
| EPA chemical information | Information provided by: ofmpub.epa.gov (external link) |
| introduction | acid black 1, also known as amino black 10B, is a diazo acid dye with a dark brown uniform powder appearance and is mainly used for dyeing leather, wool, silk, etc. Acid black 1 can be obtained by diazotization of p-nitroaniline, coupling with H acid under acidic conditions, and diazotization of aniline, coupling with the resulting monoazo compound under alkaline conditions. |
| preparation | 1) diazotization of p-nitroaniline 200 ml of water is added in a reaction cylinder, 72g of p-nitroaniline is added under stirring, 220g of hydrochloric acid is 30% in the processing industry, and the temperature is raised to 70 ℃ to make it fully soluble. Add the temperature to 0-3 ℃, and take out 5 ml for balance. Under the condition of lower than 3 ℃, add 30% sodium nitrite solution (100% 36g) at a time, stir for 15 minutes, use potassium iodide test paper to be blue, diazo solution should be a clear solution, no turbidity is allowed, the temperature is controlled at 5 ℃, and the reserved p-nitroaniline hydrochloride is gradually balanced. Control the temperature below 10 ℃ and stir. 2) Aniline diazotization Add 400 ml of water to the reaction cylinder towel, add 150 grams of human hydrochloric acid 30%, stir, add 45 grams of vinylamine to make it completely soluble, add crushed ice to cool to 3 ℃, add 30% sodium nitrite solution (34 grams of industrial products, potassium iodide test paper is blue, Congo red test paper is blue, sodium nitrite is slightly excessive, stir for 1 hour to obtain aniline diazonium salt transparent clear solution. 3)H acid dissolution H acid dissolution preparation: add 400 ml of water, add 160g of human H acid in a beaker, add 28g of soda ash water in beating, adjust the pH to 6.2, make it completely dissolve into black transparent liquid, adjust the volume to 1100 ml, let it stand for precipitation, and remove impurities. 4) One-time coupling addition of p-nitroaniline diazotization solution filtered by a fine sieve, slowly adding H acid solution within 1.5 hours below 10 ℃, slightly excessive diazonium salt, temperature 13 ℃, coupling reaction for 12 hours. 5) For the second coupling, 120 grams of flake alkali and 110 grams of soda ash are added to the above coupling liquid to make all the solvents, control the pH to 8.5, add crushed ice to cool the temperature to 5 ℃, add aniline diazonium salt solution, stir evenly, slowly adjust the pH to 8.5 with 10% soda ash solution, stir for 2 hours, measure the diazonium salt to disappear, after stirring for 1 hour, add refined salt salting according to 12% of volume, stir for 2 hours, do spot test, until it is completely cleared, filter, dry and crush 80g of finished product of standardized product acid black 1. |
| use | acid black 10B is mainly used for dyeing and direct printing of wool, silk, nylon and their blended fabrics. Poor levelness. It can be mixed with acid orange II (C.I.Acid Orange 7) to form acid black ATT with a wide range of uses. It is also used for coloring and manufacturing ink of leather, paper, soap, wood products, biology, medicine and cosmetics. It is mainly used for dyeing and printing of wool, silk, nylon and their blended fabrics. It can be combined with acid orange II to make acid black ATT, and can also be used for coloring paper, aluminum oxide, soap, wood, biology, leather, medicine and cosmetics. It can also be used to make ink. It is mainly used for dyeing and printing of wool, silk, nylon and their blended fabrics, and can also be used for color matching It is used for dyeing proteins on polyacrylamide gel, agarose gel and nitrocellulose film. After electrophoresis, it is recommended to fix the protein on the gel. The glue is dyed with 7% (v/v) acetic acid solution of 0.1% amino black 10B for at least 2 hours, and then decolorized with 7% (v/v) acetic acid solution. The detection sensitivity is about the 20% of Coomassie Brilliant Blue R250. |
| production method | p-nitroaniline diazotization, coupled with h acid to obtain monoazo compounds. Diazotization of aniline and coupling with the above monocompound to obtain raw material consumption (kg/t) h acid (100%) 240 sodium nitrite 107 p-nitroaniline (100%) 110 sodium carbonate (98%) 192 aniline (99%) 66 sodium hydroxide (100%)83 . using h acid, p-nitroaniline and aniline as raw materials, p-nitroaniline is first diazotized, coupled with h acid under acidic conditions, and then aniline is diazotized, the product is obtained by second coupling with the aforementioned coupling product under alkaline conditions. The finished product is salted out, filtered, dried and crushed.. |
| toxic substance data | information provided by: pubchem.ncbi.nlm.nih.gov (external link) |
Last Update：2024-04-09 21:11:58
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View History
sodium 4-amino-5-hydroxy-3-(4-nitrophenylazo)-6-(phenylazo)naphthalene-2,7-disulphonate
5H-Benzocycloheptene-2,3,5-triol, 6-amino-6,7,8,9-tetrahydro-, (5R,6S)-rel-
Dimethyl 3,5-Pyridinedicarboxylate
4-Amino-9,10-dihydro-1,3-dihydroxy-9,10-dioxo-2-anthracenesulfonic acid potassium salt
33414-33-4
1-(3H-imidazol-4-ylsulfonyl)-3-piperidinecarboxylic acid
29982-59-0
Kimica Algin K
N-Acetyl-L-alpha-aspartyl-L-alpha-glutamyl-N-[(1S)-1-(carboxymethyl)-3-chloro-2-oxopropyl]-L-valinamide
Benzyl Propionate
5H-Benzocycloheptene-2,3,5-triol, 6-amino-6,7,8,9-tetrahydro-, (5R,6S)-rel-
Dimethyl 3,5-Pyridinedicarboxylate
4-Amino-9,10-dihydro-1,3-dihydroxy-9,10-dioxo-2-anthracenesulfonic acid potassium salt
33414-33-4
1-(3H-imidazol-4-ylsulfonyl)-3-piperidinecarboxylic acid
29982-59-0
Kimica Algin K
N-Acetyl-L-alpha-aspartyl-L-alpha-glutamyl-N-[(1S)-1-(carboxymethyl)-3-chloro-2-oxopropyl]-L-valinamide
Benzyl Propionate