Molecular Formula | C19H19N7O6 |
Molar Mass | 441.4 |
Density | 1.4704 (rough estimate) |
Melting Point | 250 °C |
Boling Point | 552.35°C (rough estimate) |
Specific Rotation(α) | 20 º (c=1, 0.1N NaOH) |
Water Solubility | 1.6 mg/L (25 ºC) |
Solubility | Soluble in hot dilute hydrochloric acid and sulfur, slightly soluble in acetic acid, phenopyridine, hydroxide and carbonate solution, slightly soluble in methanol, insoluble in ethanol and butanol, insoluble in ether, acetone, chloroform and benzene. |
Appearance | Light orange crystal |
Color | Yellow to orange |
Odor | Odorless |
Merck | 14,4221 |
BRN | 100781 |
pKa | pKa 2.5 (Uncertain) |
Storage Condition | 2-8°C |
Stability | Stable. Incompatible with heavy metal ions, strong oxidizing agents, strong reducing agents. Solutions may be light and heat sensitive. |
Sensitive | Sensitive to light |
Refractive Index | 1.6800 (estimate) |
MDL | MFCD00079305 |
Physical and Chemical Properties | Yellow or orange-yellow crystalline powder. Odorless and tasteless. Heated to about 250 ° C, the color gradient deep, and finally into a black gum. Not soluble in water and ethanol. Slightly soluble in methanol. Soluble in acidic or alkaline solutions. |
Use | Anti-anemia drug for symptomatic or nutritional giant cell anemia. |
Risk Codes | R33 - Danger of cumulative effects R62 - Possible risk of impaired fertility R68 - Possible risk of irreversible effects |
Safety Description | 24/25 - Avoid contact with skin and eyes. |
WGK Germany | 1 |
RTECS | LP5425000 |
FLUKA BRAND F CODES | 8 |
TSCA | Yes |
HS Code | 29362900 |
Toxicity | A water soluble vitamin required in the diet of mammals. Sulfonamide drugs are selectively toxic to bacteria because they inhibit the incorporation of p-aminobenzoic acid into folic acid, a biosynthetic process in bacteria. Folic acid deficiency adversely affects prenatal development in humans. Dietary supplementation with folic acid dramatically reduces the incidence of neural tube defects in humans. Folic acid deficiency may also contribute to the causes of megaloblastic macrocytic anemia and a consequence of this is that this disease can be induced, as a side effect, when methotrexate, a folic acid antagonist, is used in cancer chemotherapy |
Reference Show more | 1. Zhang Zhenming, Chen Da, Tan Chaolan, et al. Synthesis of calcium 5-methyltetrahydrofolate by one-pot method [J]. Journal of Huaihai Institute of Technology: Natural Science Edition, 2018, 27(04):47-51. 2. Shang Yinghui, Liu Xinjun, Huang Hanchang, Lao Fengxue. Microbiological determination of folic acid in infant dairy products [J]. Journal of Food and Biotechnology, 2018,37(07):727-731. 3. Zhang Jian, Shang Yinghui, Li Tang, Liu Dongying. Comparison of the content of folic acid in the yolk of foreign and diesel eggs [J]. Journal of Beijing Union University (Natural Science Edition),2014,28(03):29-32. 4. Wang Meng, Zhang Jialing. Determination of folic acid in fruits and vegetables by fluorescence spectrometry [J]. Chinese food and nutrition, 2016, 22(03):40-42. 5. Xing Zhihua, Fang Guizhen, Su Ling, et al. Preparation and characterization of hydroxycamptothecin folate-chitosan nanoparticles [J]. Functional Materials, 2012, 43(B11):322-325. 6. Chen Yinbin, Wang Yufang, Hou Zhifang et al. Effects of Rg1 combined with B vitamins on NO release and Rg1 brain volume in mice with acute myocardial ischemia [J]. Specialty Research 2016(1):5-8. 7. Guo Gangjun, Hu Xiaojing, Xu Rong, etc. Effects of drying methods on nutrition, functional components and amino acid composition of Moringa oleifera leaves [J]. Food Science, 2018, 39(011):39-45. 8. Kai Wang. Study on the mechanism of the improvement of hepatic insulin resistance in type 2 diabetes mellitus by oral administration of heme six peptide [D]. Jilin University, 2020. 9. Mengsi Li, Caixia Wang, Liming Chen, Defang Liu,A novel electrochemiluminescence sensor based on resonance energy transfer system between nitrogen doped graphene quantum dots and boron nitride quantum dots for sensitive detection of folic acid,Analytica 10. Chen, Yin Bin, et al. "Effect of B- complex vitamins on the antifatigue activity and bioavailability of ginsenoside Re after oral administration." Journal of ginseng research 41.2 (2017): 209-214.https://doi.org/10.1016/j.jgr.2016.03.006 11. Li, Xiangyu, et al. "Folate receptor-targeting mesoporous silica-coated gold nanorod nanoparticles for the synergistic photothermal therapy and chemotherapy of rheumatoid arthritis." RSC Advances 11.6 (2021): 3567-3574.https://doi.org/10.1039/D0RA08689D 12. [IF=6.06] Yin Bin Chen et al."Effect of B- complex vitamins on the antifatigue activity and bioavailability of ginsenoside Re after oral administration."J Ginseng Res. 2017 Apr;41:209 13. [IF=2.786] Peihe Zheng et al."Influence of B- Complex Vitamins on the Pharmacokinetics of Ginsenosides Rg1, Rb1, and Ro After Oral Administration."J Med Food. 2017 Nov;20(11):1127-1132 14. [IF=9.381] Qian Du et al."A multiple environment-sensitive prodrug nanomicelle strategy based on chitosan graftomer for enhanced tumor therapy of gambogic acid."Carbohyd Polym. 2021 Sep;267:118229 15. [IF=6.558] Mengsi Li et al."A novel electrochemiluminescence sensor based on resonance energy transfer system between nitrogen doped graphene quantum dots and boron nitride quantum dots for sensitive detection of folic acid."Anal Chim Acta. 2019 Dec;1090:57 16. [IF=3.981] Wenhui Gao et al."Preparation and evaluation of folate-decorated human serum albumin nanoparticles for the targeted delivery of sorafenib to enhance antihepatocarcinoma efficacy."J Drug Deliv Sci Tec. 2019 Dec;54:101349 17. [IF=4.384] Xuena Li et al."Methotrexate-loaded folic acid of solid-phase synthesis conjugated gold nanoparticles targeted treatment for rheumatoid arthritis."Eur J Pharm Sci. 2022 Mar;170:106101 18. [IF=2.391] Wu Zhihao et al."Preparation of Monascus-fermented ginkgo seeds: optimization of fermentation parameters and evaluation of bioactivity."FOOD SCIENCE AND BIOTECHNOLOGY. 2022 Apr;:1-10 |
yellow to orange crystal or crystalline powder, odorless. Soluble in alkaline solution, carbonate solution, hydrochloric acid, sulfuric acid, acetic acid, phenol and pyridine, slightly soluble in water, insoluble in acetone, ethanol, ether and chloroform, the pH of the 10% aqueous suspension is 4.0-4.8. Stable in the air, the light is decomposed and lose physiological activity.
obtained by cyclization of 2,4, 5-triamino-6-hydroxypyrimidine, p-Aminobenzoyl glutamic acid and trichloroacetone in the presence of sodium acetate and sodium metabisulfite.
This product is N-[4-[(2-amino -4-oxo-1, 4-dihydro-6-pteridine) methylamino] benzoyl] -L-glutamic acid. The content of C19H19N706 should be between 95.0% and 102.0% based on the water content.
take this product, precision weighing, add 0.1 mol / L sodium hydroxide solution was dissolved and quantitatively diluted to prepare a solution containing about 10 mg per 1 ml, which was measured according to law (General 0621), and the specific rotation was +18 ° to +22 °.
used as a food fortifier. When folic acid is deficient, DNA synthesis must be inhibited, DNA synthesis of bone marrow erythroblasts is reduced, the rate of cell division is reduced, and the cell volume becomes larger. Mild folate deficiency may lead to depression, while severe can lead to megaloblastic anemia. Can be used for infants and young children food, the use of 380 ~ 700ug/kg; In pregnant women and lactating mothers for 2~4 mg/kg.
operation in the dark. Take about lOOmg of this product, put it in a 100ml measuring flask, add about 1ml of Ammonia Test Solution to dissolve it, dilute it to the scale with mobile phase, shake well, and use it as a test solution. Take 1ml of precision, 100ml flask, diluted with mobile phase to scale, shake, as a control solution. Take 10mg of pteroic acid, put it in a 100ml measuring flask, add 0.lmol / L sodium carbonate solution 5ml and test solution 10ml, add mobile phase to dissolve and dilute to the scale, shake, according to the chromatographic conditions under the content determination item, take l0ul injection liquid chromatograph, the resolution of pteroic acid peak and folic acid peak should be greater than 4.0. Take 10 u1 of the test solution and the control solution respectively, inject the human liquid chromatograph, record the chromatogram to 3 times of the retention time of the main component peak. In the chromatogram of the test solution, the peak areas of pteroic acid and other individual impurities shall not be greater than 0.6 times (0.6%) of the main peak area of the control solution, the sum of the peak areas of each impurity other than the pteroic acid peak shall not be greater than 2 times (2.0%) the area of the main peak of the control solution. The peaks in the chromatogram of the test solution which were 0.05 times smaller than the main peak area of the control solution were ignored.
take this product about 0.lg, precision weighing, add three gas methane-anhydrous methanol (4:l) 5ml, according to the moisture determination method (General rule 0832 first method 1), water content should not exceed 8.5%.
not more than 0.1% (General rule 0841).
vitamins.
light shielding, sealed storage.
This product contains folic acid (C19H19N7O6) should be labeled 90.0% ~ 110.0%.
This product is yellow or orange-yellow.
operation in the dark. (1) Take 20 tablets of this product (5mg specification), Precision weighing, fine grinding, precision weighing an appropriate amount (about 5mg equivalent to folic acid), put it in a 25ml measuring flask, and add about 15ml of 0.5% ammonia solution, after heating in a hot water bath for 20 minutes, the folic acid was dissolved and allowed to cool by shaking, diluted with water until the scale is reached, shaken well, filtered, and the continued filtrate was taken as the test solution, according to the method under the item of folic acid content determination, obtained.
(2) take 50 tablets of this product (0.4mg specification), Precision weighing, fine grinding, precision weighing an appropriate amount (about 4mg equivalent to folic acid), put it in a 100ml measuring flask, and add about 0.5% ammonia solution, after heating in a hot water bath for 20 minutes, the folic acid was dissolved and allowed to cool by shaking, diluted with water until the scale is reached, shaken well, filtered, and the continued filtrate was taken as the test solution, according to the chromatographic conditions under the item of folic acid content determination, take 10u1 injection liquid chromatograph for accurate measurement, record the chromatogram; Take about 10mg of folic acid reference substance, weigh it accurately, put it in 50ml measuring flask, add 0.5% ammonia solution to dissolve and dilute to the scale, shake well, take 2ml, put it in a 10ml measuring flask, dilute it with water to the scale, shake well, and measure with the same method. According to the external standard method to calculate the peak area, that is.
Same as folic acid.
(1)0.4mg (2)5mg
light shielding, sealed storage.