SD/-His/-Ade Broth - Names and Identifiers
SD/-His/-Ade Broth - Physico-chemical Properties
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SD/-His/-Ade Broth - Introduction
SD/-His/-Ade Broth(SD/-His/-Ade medium) is a specific medium for the selective cultivation or screening of amino acid deficiency-dependent yeasts in molecular biology experiments. The following is an introduction to the nature, use, formulation and safety information of SD/-His/-Ade Broth:
Nature:
- SD/-His/-Ade Broth is a colorless and transparent liquid medium.
-It contains a minimum nutrient base component (SD) that lacks histidine (His) and adenine (Ade) to inhibit the growth of yeast that is not dependent on these two amino acids.
- SD/-His/-Ade Broth will also add carbon and nitrogen sources to provide necessary nutrients.
Use:
- SD/-His/-Ade Broth is often used to selectively culture or screen proteins that interact in yeast two-hybrid assays.
-In the two-hybrid test, yeast needs to restore the autonomous synthesis ability of histidine (His) and adenine (Ade) before it can grow in SD/-His/-Ade Broth. This lack of growth characteristics is used to distinguish interacting proteins.
Preparation Method:
1. Prepare working solution of SD/-His/-Ade Broth: Dissolve SD/-His/-Ade solid medium in distilled water and prepare according to the proportion specified in the instructions.
2. Adjust the pH, usually 5.7-5.8.
3. sterilization: high temperature and high pressure sterilization or filter sterilization.
Safety Information:
1. When using SD/-His/-Ade Broth, strictly follow the laboratory's safety operation specifications.
2. When preparing the culture medium, take care to avoid skin contact, inhalation or ingestion.
3. For specific safety measures and equipment use, please refer to the SD/-His/-Ade Broth safety data sheet and laboratory safety instructions.
Last Update：2024-04-09 02:00:55