Name | Griseofulvin |
Synonyms | Grisact Grisovi grisovin Griseofulvin GRISEOFULVIN(RG) (+)-Griseofulvin Griseofulvin (200 mg) 7-Chloro-2',4,6-trimethoxy-6'b-methylspiro[benzofuran-2(3H),1'-[2]cyclohexene]-3,4'-dione 7-chloro-2',4,6-trimethoxy-6'-methyl-3H,4'H-spiro[1-benzofuran-2,1'-cyclohex[2]ene]-3,4'-dione 7-Chloro-2,4,6-trimethoxy-6-methyl-, (1S-trans)-Spiro(benzofuran-2(3H),1-(2)cyclohexene)-3,4-dione (2S,6'R)-7-chloro-2',4,6-trimethoxy-6'-methyl-3H,4'H-spiro[1-benzofuran-2,1'-cyclohexane]-3,4'-dione (2R,6'S)-7-chloro-2',4,6-trimethoxy-6'-methyl-3H,4'H-spiro[1-benzofuran-2,1'-cyclohex[2]ene]-3,4'-dione (2S,6'R)-7-chloro-2',4,6-trimethoxy-6'-methyl-3H,4'H-spiro[1-benzofuran-2,1'-cyclohex[2]ene]-3,4'-dione (2S,6'S)-7-chloro-2',4,6-trimethoxy-6'-methyl-3H,4'H-spiro[1-benzofuran-2,1'-cyclohex[2]ene]-3,4'-dione Spiro[benzofuran-2(3H),1'-[2]cyclohexene]-3,4'-dione,7-chloro-2',4,6-trimethoxy-6'-methyl-, (1'R-trans)- Griseofulvin, (2S)-trans-7-Chloro-2μ,4,6-trimethoxy-6μ-methylspiro(benzofuran-2[3H],1μ-[2]cyclohexene)-3,4μ-dione |
CAS | 126-07-8 78739-00-1 |
EINECS | 204-767-4 |
InChI | InChI=1/C17H19ClO6/c1-8-5-9(19)6-12(23-4)17(8)16(20)13-10(21-2)7-11(22-3)14(18)15(13)24-17/h7-8,12H,5-6H2,1-4H3/t8-,12?,17+/m1/s1 |
InChIKey | DDUHZTYCFQRHIY-RBHXEPJQSA-N |
Molecular Formula | C17H17ClO6 |
Molar Mass | 352.77 |
Density | 1.2579 (rough estimate) |
Melting Point | 218-220°C(lit.) |
Boling Point | 469.04°C (rough estimate) |
Specific Rotation(α) | 354 º (c=1, dimethylform) |
Flash Point | 205.9°C |
Water Solubility | practically insoluble |
Solubility | Easily soluble in tetrachloroethane, soluble in acetone or chloroform, slightly soluble in methanol or ethanol, very slightly soluble in water. |
Vapor Presure | 3.11E-11mmHg at 25°C |
Appearance | White or white-like powder |
Color | White to yellow-white |
Maximum wavelength(λmax) | ['321nm(CHCl3)(lit.)'] |
Merck | 14,4549 |
BRN | 95226 |
Storage Condition | 2-8°C |
Stability | Stable for 1 year from date of purchase as supplied. Solutions in DMSO, ethanol, or DMF may be stored at -20° for up to 3 months. |
Refractive Index | 1.4429 (estimate) |
MDL | MFCD00082343 |
Physical and Chemical Properties | White or off-white powder. Melting point 218-224 °c. Soluble in tetrachloroethane, soluble in acetone or chloroform, slightly soluble in methanol or ethanol, very slightly soluble in water. Odorless or almost odorless, slightly bitter in taste, stable to heat. |
Use | It has inhibitory effect on all kinds of dermatophytes, and is effective for the growth of fungi |
Risk Codes | R60 - May impair fertility R61 - May cause harm to the unborn child R40 - Limited evidence of a carcinogenic effect R43 - May cause sensitization by skin contact R45 - May cause cancer |
Safety Description | S53 - Avoid exposure - obtain special instructions before use. S22 - Do not breathe dust. S36/37/39 - Wear suitable protective clothing, gloves and eye/face protection. S45 - In case of accident or if you feel unwell, seek medical advice immediately (show the label whenever possible.) |
UN IDs | UN 1648 3 / PGII |
WGK Germany | 3 |
RTECS | WG9800000 |
HS Code | 29419090 |
Toxicity | LD50 oral in rat: > 10gm/kg |
Raw Materials | Oxalic acid Starch potato |
Reference Show more | 1. Ma Xiaoping, Yang Qiuxia, Yu Yan, et al. Comparison of some biological characteristics and drug sensitivity tests between wild strain Z20 and mutant strain Zt of giant panda origin [J]. Journal of Zhejiang Agriculture, 2018(8):1328-1335. 2. Peng Yan, Yang Chao, Jiang Yaozhang, isolation and Identification of Alternaria glabra from Beef Cattle [J]. Chinese Journal of Preventive Veterinary Medicine, 2020, v.42(01):86-89 99. |
This product is ([ 1 's, 6'R)-6'-methyl -2 ',4, 6-trimethoxy-7-chloro-spiro [benzofuran-2 (3H),1' 2] cyclohexene]-3,4,-Dione. Calculated as dry product, containing griseofulvin (C17H17C106) shall not be less than 95.0%.
The melting point of this product (General 0612) is 218~224°C.
take this product, precision weighing, add N ,N-dimethylformamide to dissolve and quantitatively dilute to make a solution containing about 10 mg per 1 ml, and determine according to law (General 0621), the specific rotation was 352 ° to 367 °.
take this product 0.25g, add ethanol 20ml, shake, immediately add phenolphthalein indicator solution 0.1 ml and sodium hydroxide titration solution (0.02mol/L) titration to micro red, consumption of sodium hydroxide titration solution (0.02mol/L) should not pass 1.0ml.
take 5 parts of this product, each 0.75g, respectively, plus N,N-dimethylformamide 10ml dissolved, the solution should be clear and colorless; If it is turbid, compared with No. 1 turbidity standard liquid (General rule 0902 first method), shall not be more concentrated; If color, compared with the yellow No. 3 Standard Colorimetric liquid (General rule 0901 first method), shall not be deeper.
weigh 10mg of this product accurately, add 2~4 drops of water to make it wet evenly, add 20 pieces of glass beads, shake for 3~5 minutes, then add 10ml of 5% gum arabic solution, fully shake for 10 minutes, immediately draw the Test solution from the bottom with a dropper, quickly wipe the outside of the dropper with filter paper, drop 1 drop vertically on the blood count plate, cover the cover glass, and examine it under the microscope, count, containing 5um and 5um particles should be not less than 85%, containing 50um and 50um particles, full view, not more than 5.
take an appropriate amount of this product, add mobile phase A to dissolve and dilute to make A solution containing about 0.5mg per lml as A test solution; Take 1ml for precision measurement and put it in A 100ml measuring flask, dilute to the scale with mobile phase A, shake, as A control solution; Precision take the appropriate amount of control solution, quantitative dilution with mobile phase A to make A solution containing about 0.25ug per 1ml, as solution D for sensitivity, according to the test of high performance liquid chromatography (General rule 0512), with eighteen alkyl silane bonded silica gel as filler (4.6mm X, 5um or performance equivalent column); using 0.05mol/L potassium dihydrogen phosphate solution-acetonitrile-methanol (57:38:5 )(adjust pH to 3.7±0.2 with phosphoric acid) as mobile phase A, using 0.05mol/L potassium dihydrogen phosphate solution-acetonitrile methanol (28:68:8)(adjust pH to 3.7±0.2 with phosphoric acid) as mobile phase B, immediately after the completion of the elution of griseofulvin, the linear gradient elution was carried out according to the following table; The detection wavelength was 254mn. L01, the sensitivity solution is injected into the liquid chromatograph, and the chromatogram is recorded. The signal-to-noise ratio of the peak height of the principal component chromatogram should be greater than 10, respectively injected into the liquid chromatograph, record the chromatogram. If there are impurity peaks in the chromatogram of the test solution, the peak area of deschlorin (relative retention time is about 0.7) shall not be greater than 2.5 times (2.5%) of the main peak area of the control solution, the Peak area of dehydrogriseofulvin (relative retention time is about 1.1) shall not be greater than 0.75 times (0.75%) of the main peak area of the control solution, and the sum of the peak areas of other impurities shall not be greater than the main peak area of the control solution (1.0%). The peaks in the test solution chromatogram which are smaller than the main peak area of the sensitivity test solution are ignored.
take this product, dry to constant weight at 105°C, weight loss shall not exceed 0.5% (General rule 0831).
not more than 0.2% (General rule 0841).
The residue left under the item of ignition residue shall not contain more than 25 parts per million of heavy metal when examined by law (General Principles 0821, Law II).
measured by high performance liquid chromatography (General 0512).
silica gel bonded with eighteen alkyl silane was used as a filler, and 0.05mOl / L potassium dihydrogen phosphate solution-acetonitrile-methanol (5738:5)(pH adjusted to 3.7±0.2 with phosphoric acid) was used as a mobile phase; the detection wavelength was 254nm. Take an appropriate amount of griseofulvin reference substance, add mobile phase to dissolve and dilute to make a solution containing about 0.5mg per 1 ml. Take 10u1 and inject it into human liquid chromatograph to record the chromatogram, the resolution between the dehydrogriseofulvin peak (relative retention time about 1.1) and the griseofulvin peak should meet the requirements.
take about 50mg of this product, weigh it accurately, put it in 50ml measuring flask, add mobile phase to dissolve and dilute to the scale, take 5ml accurately, put it in 50ml measuring flask, dilute to the scale with mobile phase, shake well, as a test solution, and inject 10u1 into the liquid chromatograph with precision, record the chromatogram; Take an appropriate amount of griseofulvin reference substance, and determine with the same method. According to the external standard method to calculate the peak area, that is.
antifungal drugs.
sealed storage.
This product contains griseofulvin (C17H17C106) should be labeled the amount of 90.0% ~ 110.0%.
This product is white or off-white.
In the chromatogram recorded under the content determination item, the retention time of the main peak of the test solution should be consistent with the retention time of the main peak of the control solution.
Take 10 tablets of this product, precise weighing, fine grinding, precise weighing appropriate amount (about equivalent to 100mg of griseofulvin), put it in a 100ml measuring flask, add appropriate amount of mobile phase, ultrasonic to dissolve griseofulvin, allow to cool to room temperature, dilute to scale with mobile phase, shake well; Filter with 0.45um microfiltration membrane, Take 5ml of filtrate accurately, put it in 50ml measuring flask, dilute to scale with mobile phase, shake, as a test solution, according to the method under the item of griseofulvin, obtained.
Same as griseofulvin.
(1)0.lg (2)0.125g (3)0.25g
sealed storage.