Name | Chloropyramine hydrochloride |
Synonyms | CHLOROPYRAMINE HCL Chloropyramine HCI Chloropyramine hydrochloride CHLOROPYRAMINE HYDROCHLORIDE CHLOROPYRAMINE MONOHYDROCHLORIDE N-P-CHLOROBENZYL-N,N-DIMETHYL-N-(2-PYRIDYL)ETHYLENEDIAMINE HYDROCHLORIDE N-P-CHLOROBENZYL-N',N'-DIMETHYL-N-[2-PYRIDYL]ETHYLENEDIAMINE HYDROCHLORIDE N-P-CHLOROBENZYL-N',N'-DIMETHYL-N-[2-PYRIDYL]LETHYLENEDIAMINE HYDROCHLORIDE N1-(4-Chlorobenzyl)-N2,N2-dimethyl-N1-(pyridin-2-yl)ethane-1,2-diamine hydrochloride |
CAS | 6170-42-9 |
EINECS | 228-216-2 |
Molecular Formula | C16H21Cl2N3 |
Molar Mass | 326.26 |
Melting Point | 172-174° |
Solubility | Chloroform (Slightly), Methanol (Slightly), Water (Slightly) |
Appearance | neat |
Color | White to Off-White |
Storage Condition | Inert atmosphere,Room Temperature |
Physical and Chemical Properties | White-like to white crystalline powder. Melting point 168-172 ℃. |
In vitro study | BT474 cells are highly sensitive to Chloropyramine hydrochloride (compound 1) treatment, whereby 1 µM concentrations cause a 40% reduction of viability after 48 h of treatment. It is found that at 1 µM concentrations of Chloropyramine hydrochloride, viability of control MCF7-pcDNA3 cells is significantly higher than the viability of MCF7-VEGFR-3 cells (P<0.01) and at 10 µM concentration this difference reaches twofold (P<0.001). In the BT474 cells treatment with Chloropyramine hydrochloride also leads to a concentration-dependent decrease of cell proliferation. When treatment with Chloropyramine hydrochloride is continued for 48 h, the breast cancer cells that overexpressed VEGFR-3 undergo apoptosis. This effect is dose-dependent, with 10 µM Chloropyramine hydrochloride inducing apoptosis in more than 60% of BT474 cells. In our model cell lines MCF7-pcDNA3 and MCF7-VEGFR-3, treatment with 10 µM Chloropyramine hydrochloride for 48 h leads to a 4-fold increase in apoptotic cell death in the cell line that overexpressed VEGFR-3 (18% versus 76 % respectively). |
In vivo study | Chloropyramine hydrochloride causes a dramatic reduction of tumor growth in both model systems whereby the tumor size in the treated groups is approximately 20% of the tumor size in vehicle control groups. Doxorubicin administered at 3 mg/kg causes approximately 60% reduction of tumor growth, but has no effect on tumor growth at 0, 3 mg/kg. In contrast, there is a modest effect of Chloropyramine hydrochloride alone (50% reduction of tumor growth). The low-dose combination of Chloropyramine hydrochloride and doxorubicin has a prolonged anti-tumor effect (85% reduction of tumor growth) that is greater than either drug alone. |
WGK Germany | 3 |
1mg | 5mg | 10mg | |
---|---|---|---|
1 mM | 3.065 ml | 15.325 ml | 30.65 ml |
5 mM | 0.613 ml | 3.065 ml | 6.13 ml |
10 mM | 0.307 ml | 1.533 ml | 3.065 ml |
5 mM | 0.061 ml | 0.307 ml | 0.613 ml |