Molar Mass | 38KDa(SDS-PAGE) |
Appearance | White powder |
Color | yellow |
Storage Condition | 2-8°C |
MDL | MFCD00130907 |
Use | This product is for scientific research only and shall not be used for other purposes. |
Safety Description | 24/25 - Avoid contact with skin and eyes. |
WGK Germany | 3 |
FLUKA BRAND F CODES | 3-10 |
HS Code | 35079090 |
Reference Show more | 1. Ding Junxi, Zhang Chaohua, Gao Jialong, et al. Immunomodulatory effects of ultrafiltration components from enzymatic hydrolysis products of isobarth clam meat [J]. Journal of Guangdong Ocean University, 2020, v.40(03):118-125. 2. [IF = 4.142] Chi Zhongmei et al."A photonic crystal fiber-based flux sensor for simultaneous and sensitive detection of cytoplasmic acid agents." Anal Bioanal Chem. 2022 Feb;414(4):1641-1649 |
Nearly White lyophilized powder, soluble in water or ammonium sulfate suspension. Lactate dehydrogenase derived from rabbit muscle is divided into two types: H and M. The optimum condition was 39 ℃,pH 7.4,3 × 10-3 mol.L-1 pyruvate. Very dilute solution is unstable, ammonium sulfate suspension can be stable for more than one year.
commonly used diagnostic enzymes. To test the diagnosis of pyruvate, myocardial infarction and leukemia.
0.5g/vial. The freeze-dried powder is effective for drying below 0 ℃, and the suspension is effective at about 4 ℃ to prevent freezing.
glycolytic enzyme | lactate dehydrogenase is a hydrogen receptor with oxidized coenzyme I (MADI), A dehydrogenase that catalyzes the reversible reaction between L-lactate and pyruvate. It is composed of four polypeptide chains. lactate dehydrogenase (LDH) is a glycolytic enzyme. Lactate dehydrogenase exists in the cytoplasm of all tissue cells of the body, and the content of the kidney is higher. Lactate dehydrogenase is an enzyme that catalyzes the production of lactate from pyruvate and is found in almost all tissues. There are six kinds of isozymes, namely LDH-1(H4), LDH-2(H3M), LDH-3(H2M2), LDH-4(HM3), LDH-5(M4) and LDH-C4, they can be separated by electrophoresis. The distribution of LDH isozymes is clearly tissue-specific, so that disease can be co-diagnosed according to their tissue-specificity. LDH2,> LDH1 in normal human serum. If myocardial enzymes are released into the blood, LDH1> LDH2 can be used to observe and diagnose myocardial diseases. The molecular weight of lactate dehydrogenase (LDH) was 130-140KDa, It consists of two subunits: H (for heart) and M (for muscle). They are arranged in different forms to form five isozymes containing four subunits, namely: LDH1(H4), LDH2(H3M1), LDH3(H2M2), LDH4(HM3), LDH5(M4). LDH catalyzes the reduction and oxidation reaction between pyruvate and lactate, and promotes the reaction of lactic acid to pyruvic acid under alkaline conditions, the conversion of pyruvic acid to lactic acid was promoted under neutral conditions (as a reverse reaction). LDH is an important enzyme involved in anaerobic glycolysis and gluconeogenesis of sugars. since LDH is present in almost all somatic cells and is generally highly active in human tissues, the increase of LDH in serum is not specific to any single tissue or organ. In AMI elevated late, peak late, so the early diagnosis of little value. Because of the long half-life (10 to 163 hours), it is often used for retrospective diagnosis, such as AMI patients hospitalized later, diagnosis of subacute MI and disease monitoring. The distribution of LDH in tissues was mainly in heart and kidney with LDH1, followed by LDH2; Lung with LDH3.LDH4; Skeletal muscle with LDH5; Liver with LDH5, LDH4. The order of serum LDH content is LDH2>LDH1>LDH3>LDH4>LDH5. |
uses | lactate dehydrogenase is widely present in various tissues, with the highest content in myocardium, liver, skeletal muscle and lung, its activity is about 500 to 1000 times that of serum. If the organ or tissue is damaged, the enzyme can be released into the blood, and the serum level of this enzyme is increased, but because of its wide distribution, it lacks specificity. Elevated serum levels of this enzyme are mainly seen in acute myocardial infarction, viral myocarditis, liver cirrhosis, pulmonary infarction, malignant tumors and leukemia. |