Appearance | suspension |
Color | white |
Merck | 13,5349 |
Storage Condition | 2-8°C |
Physical and Chemical Properties | A crystalline suspension of white-like freeze-dried powder or 3.2mol/L ammonium sulfate, soluble in water, pI is 4.6, and the optimum pH value is 6.0. Stability: The crystallase solution can be stored for 6-8 weeks, the activity remains unchanged, the sulfate suspension can be stable for more than one year, and the extremely dilute solution is unstable; the optimum temperature is 39 ℃, and the temperature is higher than 60 ℃. Inhibitors include excess pyruvate, excess NAD, oxalic acid, oxalic acid mono-amide, hydroxymalonic acid, malonic acid, urea, Cu2, Hg2, Ag, 4-chloromirilate benzoic acid, pyrophosphate or phosphate buffer and non-competitive inhibitors formed from coenzyme I (NAD) when alkaline have an inhibitory effect on the oxidation reaction, excessive lactic acid and non-competitive inhibitors formed from reduced coenzyme I (NADH) have an inhibitory effect on the reduction reaction; the activators are 2-amino-2-methyl-1-propanol, fluoride, diethanolamine and heparin (oxidation). Enzyme reaction: pyruvate reduction of coenzyme I H-lactate coenzyme I. |
Risk Codes | R21/22 - Harmful in contact with skin and if swallowed. R36/37/38 - Irritating to eyes, respiratory system and skin. |
Safety Description | S22 - Do not breathe dust. S24/25 - Avoid contact with skin and eyes. S36/37 - Wear suitable protective clothing and gloves. S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. |
WGK Germany | 3 |
FLUKA BRAND F CODES | 3-10 |
HS Code | 35079090 |
Reference Show more | 1. [IF = 4.142] Chi Zhongmei et al."A photonic crystal fiber-based flux sensor for simultaneous and sensitive detection of cytoplasmic acid agents." Anal Bioanal Chem. 2022 Feb;414(4):1641-1649 Note: For some products, we can only provide some information, and we do not guarantee the authority of the information provided, for customer reference and communication purposes only. storage conditions: -20 °c Appearance: white powder inhibitor: Co2 Cu2 ,Fe3 ,Ni2 ,Zn2 ,NEM,SDS,Proclin optimal temperature: 45 ℃ Thermal stability:<50 ℃(PH7.4,15min) optimal PH:6.5 stability: 4.5~10.0(37 ℃,1hr) Molecular Weight: 38KDa(SDS-PAGE) activity: ≥ 300U/mg protein vitality definition: One unit will convert one micromole of pyruvate to L-lactate per min at pH 7.4 at 25 °c. enzyme code: EC 1.1.1.27 |
EPA chemical substance information | information provided by: ofmpeb.epa.gov (external link) |
Overview | L-lactate dehydrogenase is a glycolytic enzyme found in the cytoplasm of all tissues and cells of the body, among them, the content of kidney is higher. L-lactate dehydrogenase is an enzyme that catalyzes the production of lactate from pyruvate and is present in almost all tissues. In animals or microorganisms, lactate dehydrogenase (LDH) is a key enzyme in the fermentation of lactic acid bacteria, which uses NADH as a coenzyme and generates lactic acid from pyruvate through biochemical reaction. |
preparation method | add 300μL bacterial suspension (30mg wet cell) and 250μL sodium phosphate buffer (pH 7.0) into a 1ml centrifuge tube, 37 ℃ for 5min, followed by adding 50 μL glucose (final concentration of 50mmol/L) and 400 μl Phenylpyruvic acid (final concentration of 10mmol/L), 200 μl of reaction solution was added to 800 μl of methanol to terminate the reaction, and the reaction was analyzed by HPLC through 0.22 μm organic filter membrane. Another 200 μl of reaction solution was extracted with 1ml of ethyl acetate, the upper organic phase was dried over anhydrous sodium sulfate and then subjected to a 0.22 μm organic filter for enantioselectivity analysis. The results showed that when the reaction time was 40min, the concentration of L-PLA was 5.62mmol/L, the yield was 56.2%,eep>99%, when the reaction time was 80min, Phenylpyruvic acid was consumed completely, L-lactate dehydrogenase was produced at 8.59mmol/L with a yield of 85.9% and eep>99%. |
Use | for biochemical studies, pyruvate assay, diagnosis of leukemia and myocardial infarction. commonly used in the diagnosis of myocardial infarction and leukemia. Assay for pyruvate. |
production method | using pig heart as raw material, calcium phosphate gel treatment, ammonium sulfate precipitation, dialysis, the product was obtained by crystallization of ammonium sulfate. in order to avoid muscle as raw material, after calcium phosphate gel treatment, ammonium sulfate classification precipitation, and then by dialysis, ammonium sulfate crystallization to get the product. |