Name | Resazurin, sodium salt |
Synonyms | Resazurin Resin Azure DIAZORESORCINOL Resazurin sodium Resazurin Sodium Salt Resazurin, sodium salt RESAZURIN SODIUM SALT(RG) resazurin,sodiumderivative 7-sodiooxy-3H-phenoxazin-3-one 10-oxide sodium 3-oxo-3H-phenoxazin-7-olate 10-oxide 3H-Phenoxazin-3-one,7-hydroxy-,10-oxide,sodiumsalt 7-Hydroxy-3H-phenoxazin-3-one 10-oxide sodium salt 7-Hydroxy-3H-phenoxazin-3-one 10-oxide, sodium salt RESAZURIN, SODIUM SALTRESAZURIN, SODIUM SALTRESAZURIN, SODIUM SALT |
CAS | 62758-13-8 |
EINECS | 263-718-5 |
InChI | InChI=1/C12H7NO4.Na/c14-7-1-3-9-11(5-7)17-12-6-8(15)2-4-10(12)13(9)16;/h1-6,14H;/q;+1/p-1 |
InChIKey | IVGPGQSSDLDOLH-UHFFFAOYSA-M |
Molecular Formula | C12H8NNaO4 |
Molar Mass | 253.19 |
Melting Point | >250℃ |
Boling Point | 477.2°C at 760 mmHg |
Flash Point | 242.4°C |
Water Solubility | soluble |
Solubility | Soluble in water and 1 M ammonium hydroxide solution (1 mg/ml); Slightly soluble in ethanol/glacial acetic acid/DMSO(< 0.5 mg/mL);(Dissolve in alkali to obtain blue solution, dissolve in alcohol to show brick red fluorescence) |
Vapor Presure | 9.94E-10mmHg at 25°C |
Appearance | Dark green to black powder |
Color | Dark green to black |
Odor | Odorless |
Maximum wavelength(λmax) | ['598 nm, 380 nm'] |
Merck | 14,8141 |
BRN | 3794971 |
pKa | 6.71(at 25℃) |
Storage Condition | room temp |
Stability | Stable. Incompatible with strong oxidizing agents. |
MDL | MFCD00005036 |
Use | This product is for scientific research only and shall not be used for other purposes. |
In vitro study | Resazurin sodium (Diazoresorcinol sodium) is commonly used to measure bacterial and eukaryotic cell viability through its reduction to the fluorescent product resorufin. No viable bacteria are detected 24 h post-inoculation following inclusion of Resazurin sodium in TSBc cultures of F. tularensis LVS at the concentration of 44 μM. Lowering the Resazurin sodium concentration to as little as 4.4 μM still results in a 10-fold reduction in viable F. tularensis LVS compare to growth medium alone. Both Resazurin sodium treatments result in a significant decrease in viable F. tularensis LVS bacteria over 22 h. Treatment with Resazurin sodium significantly reduces the number of viable F. tularensis LVS bacteria in HEK293 cells 22 h post-infection. |
Risk Codes | R36/37/38 - Irritating to eyes, respiratory system and skin. R22 - Harmful if swallowed |
Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36/37/39 - Wear suitable protective clothing, gloves and eye/face protection. S36 - Wear suitable protective clothing. |
WGK Germany | 3 |
RTECS | SP7700000 |
TSCA | Yes |
HS Code | 29349990 |
Reference Show more | 1. Feng Wen-Wen, Wu Shan Gong, Hao Yao Tong, etc. Isolation and identification of anaerobic bacteria from intestinal mucosa of grass carp. 2. Wang Xinchun, Xu Wei, Jiang Jianxin, etc. Study on in vitro metabolic process of oxidative degradation products of wild Gleditsia sinensis polysaccharide [J]. Chemistry and Industry of forest products, 2020(3). 3. Lin, Li Chunmei. Effects of Persimmon tannin on intestinal microbes in simulated fermentation environment in vitro [J]. Science and Technology of food industry,, 36(022):160-163,167. 4. Cheng Yuxin. Regulatory effect and mechanism of blueberry pomace fermented by Lactobacillus casei on intestinal barrier function in mice fed with high fat diet [D]. Huazhong Agricultural University, 2020. 5. [IF = 5.64] Fengyu Guo et al."Antimicrobial Activity and Proposed Action Mechanism of Linalool Against segomonas fluorescens." Front Microbiol. 2021 Jan 28;12:562094 6. [IF = 2.629] Jiang Li et al."Phenolics and Carbohydrates in Buckwheat Honey Regulate the Human Intestinal Microbiota."Evid-Based Compl Alt. 2020;2020:6432942 7. [IF=5.645] Shenglan Liao et al."Chemical composition of Zanthoxylum schinifolium Siebold & Zucc. essential oil and evaluation of its antifungal activity and potential modes of action on Malassezia restricta."Ind Crop Prod. 2022 Jun;180:114698 |
pH range of acid-base indicator discoloration | Orange (3.8) to dark violet (6.5) |
Biological applications | Detecting bilecaccids, glycosidase activity; as hydroase substrate; in beer production; enzymatic microplate assay; b-glucosidase assay; glutathione transferase assay |
Main applications | Electronic solutions/devices (mirrors, glazings, partitions, filters, displays, lenses);oxygen-barrier packaging materials; oxidation-reduction (redox) indicator |
EPA chemical substance information | information provided by: ofmpeb.epa.gov (external link) |
biological activity | Resazurin sodium it is commonly used to measure the viability of bacterial and eukaryotic cells by reducing it to the fluorescent product, trehaline. |
Use | is used as a cell stain. Also useful as an acid-base indicator, pH 3.8 (orange) to 6.5 (dark purple). It is used in food research as a reductive indicator for testing reductase, assaying hyposulfate and examining bacteria in milk. used as acid-base indicator, pH 3.8 (orange) -6.5 (dark purple). It is used in food research as a reductive indicator for testing reductase, assaying hyposulfate and examining bacteria in milk. Biological staining tests such as quantitative detection of cell proliferation and toxicity. |