Molecular Formula | C28H34O9 |
Molar Mass | 514.56 |
Density | 1.28 |
Melting Point | 97-99°C |
Boling Point | 638.6±55.0 °C(Predicted) |
Specific Rotation(α) | -50°(c=1.12,chloroform) |
Flash Point | 206.4°C |
Solubility | DMSO : 250 mg/mL (485.85 mM; Need ultrasonic) |
Vapor Presure | 3.51E-17mmHg at 25°C |
Appearance | White powder |
Color | White to Off-White |
pKa | 13.54±0.60(Predicted) |
Storage Condition | Refrigerator |
Refractive Index | 1.588 |
MDL | MFCD08460221 |
Physical and Chemical Properties | Form: white powder natural product structure type: lignans density: 1.28±0.1g/cm3 (predicted) Boiling Point: 638.6±55.0°C (predicted) Melting Point: 97-99°C pKa:13.54±0.60 (predicted) |
Reference Show more | 1. Jiao Meiyu, Yu-yue, Wu Haoxian, Kang Tingguo, Zhang Hui. Study on the mechanism and qualitative recognition model of "color discrimination and qualitative analysis" of schisandra chinensis [J]. China Pharmacy, 2020,31(24):3007-3012. 2. [IF = 4.759] Huifen Ma et al."The in-capsule -2,2-azinobis-(USB acid)." J Ch 3. [IF = 3.935] Yunlong Guo et al."Rapid characterization of Schisandra species by using direct analysis in real time mass spectroscopy." J Pharmaceut Biomed. 2021 Jan;192:113648 4. [IF = 4.36] Zhenghua Wu et al."Schisandrol A, the main active ingredient of Schisandrae Chinensis Fructus, inhibits pulmonary fibrosis through suppression of the TGF-β signaling pathway as revealed by UPLC-Q-TOF/MS, network pharmacology and experimental verification. |
Overview | schisandrin B is one of the common lignans in schisandra, the 2010 edition of Chinese Pharmacopoeia income of schisandra varieties for schisandra (schisandra) and schisandra (Central schisandra). With liver protection, reduce alanine aminotransferase and other activities. |
plant origin | schisandrae schisandrae B is mainly derived from schisandra plants of the Magnoliaceae family. There are about 25 species of this genus in the world, mainly distributed in East Asia and a few in southeast America. China is the center of the distribution of this genus. There are 18 species of schisandra plants in China, including about 13 species of medicinal. Schisandra chinensis (Turcz.)Baill. The medicinal part is dry and mature fruit. In autumn, when the fruit is ripe, it is picked and dried to remove the fruit stem and impurities. schisandra chinensis grows in mountain trees, distributed in Liaoning, Jilin, Heilongjiang, Inner Mongolia, Beijing and Hebei. Schisandra sphenanthera was born in the wet broad-leaved forest or Bush, mainly distributed in Shaanxi, Henan, Gansu, and Hunan, Hubei, Zhejiang, Sichuan, Yunnan, Jiangsu. Figure 1 shows the schisandra plants and medicinal materials. |
Chemical composition | the chemical composition of schisandra chinensis mainly contains lignans, volatile oil, polysaccharide, organic acid and other components, among them, Lignans are the main active components. Volatile Components fruit contains about 3% of volatile oil. Contains α-pinene (2-pinene), β-pinene (β-pinene), myrcene, limonene, α-terpinene (α-lerpinene), r-terpinene (r-terpinene), thymol methyl ether, linalool, etc. Lignans about 200 lignans have been isolated from Schisandraceae plants at home and abroad, of which more than 150 are lignans. Schisandra chinensis contains Schisandrol, schisandrin (schisandrin), schisandrin (schisandrin), schisandrin (aomitin) A, schisandrin (deoxyschisandrin) Schisandrin B is r-schisandrin (r-Schizandrin), schisandrin C is pseudo r-schisandrin (Pseudo-r-Schizandrin), also contains Schisanhenol, SchisantherinA, schisandra chinensis Ester, et al. schisandra sphenanthera mainly contains schisandrin A, schisandrin B and schisandrin A, a small number of varieties containing schisandrin A and schisandrin B, and deoxyschisandrin schisandrin, schisandrin (neoschisan-drin), schisandrin (schizandrol), golisin, etc. Organic acids citric acid, citric acid, malic acid, tartaric acid, succinic acid, protocatechuic acid, etc. The glycosides include thymol 5-o-β-d-glucopyranoside, kaempferol and 3-o-β-rutin. Other sterols, resin, vitamins, tannins, chlorophyll, a small amount of sugar. |
factors affecting the content of lignans and schisandra chinensis Ester B | 1. Factors affecting the content of lignans in schisandra chinensis 1. Selection of varieties: the content of total lignans in different varieties of schisandra chinensis is quite different, and the content of schisandra chinensis is the highest. 2. Choice of place of origin: schisandra chinensis and schisandra sphenanthera seeds in different regions of the content of lignans is quite different, schisandra chinensis to the highest content in Shanxi, alcohol amount of 9.87%, lignans total sum of 22.7%, in addition to the content of lignans in Jinshan, Xingshan, the lower content of schisandra, other varieties were more than 2%. Southern schisandra with sukang county, Hubei, a higher content. 3. Selection of harvesting time: schisandra chinensis is usually harvested from bright red to purple after ripening in August to September. There were differences in the content of lignans in different growth stages of schisandra chinensis. In the whole year, the content of lignans is higher from May to 7 months, only from the point of view of the content, the harvest period is 7 months, but considering the biomass and effective component content of the fruit, harvesting is usually done in September to October (after frost fall). 4. Harvest of alternative parts: the contents of total lignans and three effective components in schisandra chinensis stem were the highest in 7 months, so 7 months was the best harvest time of schisandra chinensis stem. The content difference of schisandrin B in schisandra chinensis from north to south UPLC/Q-TOF-MS method was used to analyze the lignans in the extract of schisandra chinensis. In the positive ion mode, the lignans of schisandra sphenanthera and schisandra sphenanthera were compared and analyzed by using the similarities and differences of the mass spectrometry behavior of lignans, schisandra sphenanthera and schisandra chinensis have some identical chemical composition, namely schisandrin A, schisandrin B, schisandrin A, schisandrin B, schisandrin B, schisandrin C, schisandrin C, schisandrin A, but the content is quite different, Schisandra chinensis in alcohol methyl, alcohol ethyl, Ester butyl content is higher, and schisandra sphenanthera ester methyl, ethyl ester, propyl ester, a content. |
determination of lignans content in schisandra chinensis | : 2010 edition of "Chinese Pharmacopoeia" Schisandra and schisandra sphenanthera in the "content determination" components were schisandrin and schisandrin Ester A, schisandrin ethyl ester content determination did not involve. The lignans in the extract of schisandra chinensis were analyzed by spectrophotometry. Preparation of standard curve: precision weighing schisandrin B reference product 30mg in 5ml volumetric flask, plus chloroform dissolved and fixed volume to the scale, put in the refrigerator for standby, with a micro syringe respectively draw standard solution 2, 4, 6, 8, 10, 12, 14, 16 μl were injected into a 10ml test tube, while a test tube was blank with 16 μl chloroform, the chloroform was evaporated in a water bath, and 10% of 0.5 aqueous chromotropic acid solution was added, concentrated sulfuric acid 3.0ml, distilled water 1.5ml, carefully mixed, boiling water bath heating for 30 minutes, take out and cool, ultraviolet spectrophotometer at 570nm absorbance. The absorbance value was taken as the ordinate, and the amount of schisandrin B (μg) was taken as the abscissa to draw the standard working curve or calculate the regression equation. Sample determination: accurately weigh 0.1500g of extract, put it in a 10ml volumetric flask, add 8ml of 95% ethanol for ultrasonic dissolution, cool and fix the volume, accurately draw 1ml of the above ethanol solution, stir in 1g of silica gel, volatilize dry ethanol, Fill the silica gel into a small layer of absorbent cotton pre-packed column at the bottom of the column, wash it with chloroform into a 10ml volumetric flask and reach the scale, accurately absorb the above chloroform eluent 0.5ml in a 10ml test tube, distilled off chloroform, with the standard curve item from "add 10% aqueous solution of chromotropic acid 0.5..." Operate according to law. The number of micrograms corresponding to the absorbance value is found on the standard curve or calculated according to the regression equation. Note: the principle of this method is that schisandrin B, schisandrin B, schisandrin B, C and other chemical structures all contain methylenedioxy groups, in the presence of concentrated sulfuric acid, methylenedioxy group can be hydrolyzed to release formaldehyde, and the released formaldehyde can react with chromotropic acid to produce purple red, with maximum absorption at 570nm wavelength. |
pharmacological effects | 1. Protective effect on liver schisandra chinensis Extract and its preparations can promote gluconeogenesis in liver, and can promote the decomposition of liver glycogen, and the brain, liver, muscle of fructose glucose phosphorylation process; The ethanol extract can significantly reduce the serum alanine aminotransferase, it has a protective effect on liver injury caused by carbon tetrachloride. The lignans of schisandra chinensis have different degrees of liver-protecting and enzyme-reducing effects, and are the main components of various liver-protecting and enzyme-reducing Chinese patent medicines on the market at present. 2. Effect on digestive tract ulcer schisandra chinensis Extract has a better inhibitory effect on gastric ulcer disease, and also has a preventive effect on gastric mucosal injury caused by anhydrous ethanol. schisandra chinensis contains a variety of active ingredients, which can reduce the damage of oxygen free radicals to myocardial cells and inhibit the infiltration of neutrophils, it has a protective effect on myocardial ischemia reperfusion injury. It can also relax blood vessels, reduce blood pressure, regulate myocardial cell energy metabolism, reduce myocardial contractility, reduce blood lipid, and produce anti-platelet aggregation effect. 4. Effects on central nervous system schisandra chinensis has many effects on central nervous system, including sedative and hypnotic effects, anticonvulsant effects, and nootropic effects. 5, the role of the genitourinary system schisandra chinensis has the effect of nourishing the kidney, and schisandrin B has a great role in promoting the oxidative damage of rat kidney caused by sub-chronic exposure to cadmium. The antitumor effects of total lignans on human breast cancer cell line MCF-7, hepatoma cell line HepG2 and human esophageal cancer cell line 9706 in vitro were determined by MTT method, the total lignans of schisandra chinensis have certain anti-tumor activity in vitro. 7. Antioxidant effect A variety of lignans in schisandra chinensis has significant antioxidant effect, schisandra chinensis and contained a variety of lignans compounds in animals with inhibition and scavenging a variety of free radicals, enhance the activity of SOD and other antioxidants in the body. 8, other effects antibacterial effect, renal protection, anti-allergic effect, promote glucose metabolism, choleretic effect, strengthen uterine contraction, enhance eye and ear and skin function. |
pharmacological activity | schisandrin B can reduce serum alanine aminotransferase. Mice were orally administered 50mg/kg per day, and the activity of liver alanine aminotransferase was 4.7, while the control group was 116, which was significantly decreased. In mice due to carbon tetrachloride and thioacetamide poisoning SgPT increased, there is a strong reducing effect. The dosage of 100mg/kg could prolong the sleep time of mice, and the effect of intraperitoneal injection was stronger than that of gavage. |
schisandra clinical application | 1. Treatment of neurasthenia and neurosis. 2. Treatment of acute chronic hepatitis and elevated transaminases caused by psychotropic drugs, as well as cirrhosis. 3. Treatment of chronic bronchitis, emphysema, Cough, shortness of breath. 4. Treatment of physical weakness, fatigue, Fatigue, Palpitations, sweating and other symptoms. |