Name | Diosmin |
Synonyms | DIOSMIN Diosmin Diovenor DIOSIMIN BAROSMIN BAROSMIN hplc DIOSMETIN-7-RUTINOSIDE DIOSMETIN-7-O-RUTINOSIDE 3,5,7-Trihydroxy-4-methoxyflavone-7- rutinoside 3',5,7-trihydroxy-4'-methoxyflavone 7-rutinoside 3',5-Dihydroxy-4'-methoxy-7-(6-O-α-L-rhamnopyranosyl-β-D-glucopyranosyloxy)flavone 5-hydroxy-2-(3-hydroxy-4-methoxyphenyl)-4-oxo-4H-chromen-7-yl 6-O-(6-deoxyhexopyranosyl)hexopyranoside 5-hydroxy-2-(3-hydroxy-4-methoxyphenyl)-4-oxo-4H-chromen-7-yl 6-O-(6-deoxy-alpha-D-mannopyranosyl)-beta-D-glucopyranoside 5-hydroxy-2-(3-hydroxy-4-methoxyphenyl)-4-oxo-4H-chromen-7-yl 6-O-(6-deoxy-alpha-L-mannopyranosyl)-beta-D-glucopyranoside |
CAS | 520-27-4 |
EINECS | 208-289-7 |
InChI | InChI=1/C28H32O15/c1-10-21(32)23(34)25(36)27(40-10)39-9-19-22(33)24(35)26(37)28(43-19)41-12-6-14(30)20-15(31)8-17(42-18(20)7-12)11-3-4-16(38-2)13(29)5-11/h3-8,10,19,21-30,32-37H,9H2,1-2H3/t10-,19-,21-,22-,23+,24+,25+,26-,27+,28-/m1/s1 |
InChIKey | GZSOSUNBTXMUFQ-YFAPSIMESA-N |
Molecular Formula | C28H32O15 |
Molar Mass | 608.54 |
Density | 1.68±0.1 g/cm3(Predicted) |
Melting Point | 277-278°C |
Boling Point | 926.8±65.0 °C(Predicted) |
Specific Rotation(α) | (C=0.5, 0.1mol/L NaOH)-121 |
Flash Point | 305.2°C |
Water Solubility | Soluble in DMSO (50 mg/ml), water (122 mg/ml at 25°C), and ethanol (<1 mg/ml at 25°C). |
Solubility | Almost insoluble in water, soluble in dimethyl sulfoxide, almost insoluble in ethanol. Soluble in a dilute alkaline solution. |
Vapor Presure | 0Pa at 20℃ |
Appearance | gray or yellowish hygroscopic powder |
Color | Pale Beige to Brown |
Maximum wavelength(λmax) | ['345nm(EtOH)(lit.)'] |
Merck | 14,3297 |
pKa | 6.10±0.40(Predicted) |
Storage Condition | Sealed in dry,2-8°C |
Refractive Index | 1.711 |
MDL | MFCD00009772 |
Physical and Chemical Properties | It is soluble in dimethyl sulfoxide and strong alkaline solution, insoluble in water, methanol or ethanol, and slightly soluble in 0.1mol/L sodium hydroxide. |
Use | For food, medicine, health care products, etc |
Hazard Symbols | Xi - Irritant |
Risk Codes | 36/37/38 - Irritating to eyes, respiratory system and skin. |
Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36 - Wear suitable protective clothing. S36/37 - Wear suitable protective clothing and gloves. S24/25 - Avoid contact with skin and eyes. |
WGK Germany | 3 |
HS Code | 29381000 |
Reference Show more | 1. Liu Xuehui, Xie Hongqi, Zhang Si, etc. Separation of lignosulfonate from Australian Mint by high-speed countercurrent chromatography [J]. Chinese agricultural bulletin, 2016, 32(027):109-113. 2. Xing-Yan, L. I. U., et al. "Identification of active compound combination contributing to anti-allergic activity of Xiao-Cheng-Qi." Chinese journal of natural Medicine 16.7 (2018): 513-524.https:// do 3. Tang W, Li W, Yang Y, Lin X, Wang L, Li C, Yang R. Phenolic Compounds Profile and Antioxidant Capacity of Pitahaya Fruit Peel from Two Red-Skinned Species (Hylocereus polyrhizus and Hylocereus undatus). Foods. 2021; 10(6):1183. https://doi.org/10.3390/food 4. [IF=6.529] Xing-Yan Liu et al."The activity of Hou-Po-Da-Huang-Tang is improved through intestinal bacterial metabolism and Hou-Po-Da-Huang-Tang selectively stimulate the growth of intestinal bacteria associated with health."Biomed Pharmacother. 2017 Oct;94:794 5. [IF=3] Xing-Yan LIU et al."Identification of active compound combination contributing to anti-inflammatory activity of Xiao-Cheng-Qi Decoction via human intestinal bacterial metabolism."Chin J Nat Medicines. 2018 Jul;16:513 6. [IF=6.543] Wang Yue et al."Polymethoxyflavones in Citrus Regulate Lipopolysaccharide-Induced Oscillating Decay of Circadian Rhythm Genes by Inhibiting Nlrp3 Expression."Oxid Med Cell Longev. 2021;2021:8419415 7. [IF=4.35] Wanpei Tang et al."Phenolic Compounds Profile and Antioxidant Capacity of Pitahaya Fruit Peel from Two Red-Skinned Species (Hylocereus polyrhizus and Hylocereus undatus)."Foods. 2021 Jun;10(6):1183 8. [IF=5.34] Dong Shao et al."Identification of the active compounds and functional mechanisms of Jinshui Huanxian formula in pulmonary fibrosis by integrating serum pharmacochemistry with network pharmacology."PHYTOMEDICINE. 2022 Jul;102:154177 9. [IF=6.576] Junkun Pan et al."Inhibition of Dipeptidyl Peptidase-4 by Flavonoids: Structure-Activity Relationship, Kinetics and Interaction Mechanism."Frontiers in Nutrition. 2022; 9: 892426 |
This product is 7-[[6-0 (6-deoxy-a-L-manranosyl)-B-D-glucopyranosyl] oxy] -5-hydroxy-2-(3-hydroxy-4-methoxyphenylbenzopyran-4-one. The content of diosmin (C28H32015) shall not be less than 90.0% based on the dry product.
take 2.0g of this product, add water 80ml, shake, filter, take the filtrate 40ml, check according to law (General rule 0802), and standard potassium sulfate solution 1.0ml of the control solution should not be more concentrated (0.01%).
take the test solution under the item of related substances as the test solution; Take 25mg of hesperidin reference substance, weigh it accurately, put it in a 50ml measuring flask, add dimethyl sulfoxide to dissolve and dilute it to the scale, shake, precision take 5ml, 50ml flask, diluted with dimethyl sulfoxide to the scale, shake, as a reference solution. According to the chromatographic condition test under the content determination item, take 10 u1 of each of the reference solution and the test solution, inject the human liquid chromatograph respectively, record the chromatogram, and calculate the peak area according to the external standard method, containing hesperidin not more than 5.0%.
take 25mg of this product, precision weighing, put it in 25ml measuring flask, add dimethyl sulfoxide to dissolve and dilute to the scale, shake, as a test solution; Take about 10mg of diosmin reference substance, add dimethyl sulfoxide to dissolve and dilute to the scale, shake well, take 3ml of precision weighing, put it in 100ml measuring flask, dilute it to the scale with dimethyl sulfoxide, shake well, as a control solution. The appropriate amount of the reference solution was Diluted 20 times with dimethyl sulfoxide as a sensitivity solution. According to the chromatographic condition test under the content determination item, 10ul of the sensitivity solution is injected into the liquid chromatograph, and the signal-to-noise ratio of the peak height of the main component should be greater than 10; Then each Lul of the reference solution and the test solution is accurately measured, they were injected into the liquid chromatograph, and the chromatogram was recorded to 5 times of the retention time of the main peak. If there are impurity peaks in the chromatogram of the test solution, the content of a single impurity shall not be more than 3.0%, and the content of a single impurity in the range of 1.0%-3.0% shall not be more than 3, calculated based on the peak area except hesperidin, the total amount of impurities should not exceed 8.0%. The chromatographic peaks in the chromatogram of the test solution which are smaller than the main peak area of the sensitivity solution are ignored (0.15%).
take an appropriate amount of this product, weigh it accurately, add dimethyl sulfoxide to dissolve and dilute to make a solution containing 40mg per lml, as a test solution; Take methanol, pyridine and N, an appropriate amount of N-dimethylformamide was precisely weighed and diluted with dimethyl sulfoxide to prepare a solution containing 120UG, 8UG and 35.2ug per 1 ml, respectively, as a reference solution. According to the test for determination of residual solvents (General Principle 0861 third method), the capillary column with 6% cyanopropyl phenyl-94% dimethyl polysiloxane (or similar polarity) as stationary liquid is used as the chromatographic column, and the initial temperature is 70°C, it was maintained for 5 minutes; The inlet temperature was 250°C; The detector temperature was 250°C, and the temperature was raised to 230°C at a rate of 25°C per minute for 5 minutes. The lul of the reference solution and the test solution were respectively injected into the gas chromatograph, and the chromatogram was recorded. According to the external standard method, the residual amount of methanol, pyridine and N,N-dimethylformamide shall be in accordance with the provisions.
take this product, dry to constant weight at 105°C, weight loss shall not exceed 5.0% (General rule 0831).
take l.Og of this product and check it according to law (General rule 0841). The residue left shall not exceed 0.5%.
The residue left under the item of taking the ignition residue shall not contain more than 20 parts per million of heavy metal when examined by law (General rule 0821, Law II).
measured by high performance liquid chromatography (General 0512).
silica gel bonded with octa-alkyl silane as filler (phenenex C18,4.6mm X 100mm , 3um or equivalent column); Water-methanol-glacial acetic acid-acetonitrile (66:28:6:2) as mobile phase, the detection wavelength was 275nm. Add about 10 mg of diosmin to 10ml measuring flask, add dimethyl sulfoxide to dissolve and dilute to the scale, shake well, use as solution (1), and add about 10 mg of hesperidin to 10ml measuring flask, add dimethyl sulfoxide to dissolve and dilute to the scale, shake the sentence, take lml and solution (l) 3ml in the same 100ml measuring flask, dilute to the scale with dimethyl sulfoxide, shake, as the system applicable solution, 10u1 injection liquid chromatography, record chromatogram, hesperidin peak and diosmin peak separation degree should be greater than 4.6.
take about 20mg of this product, accurately weigh it, put it in a 100ml measuring flask, add dimethyl sulfoxide to dissolve and dilute to the scale, shake it well, and use it as a test solution, A sample of 10u1 was injected into human liquid chromatograph, and the chromatogram was recorded. According to the external standard method to calculate the peak area, that is.
capillary protective drugs.
sealed and stored in a dry place.
This product contains diosmin (C28H32015) should be 90.0% ~ 110.0% of the label amount.
This product is a film-coated tablet, which is pale yellow to brown yellow after removing the coating.
Same as diosmin.
0.45g
sealed storage.