Molecular Formula | C43H49N2NaO6S2 |
Molar Mass | 776.98 |
Melting Point | 235 °C |
Water Solubility | slightly soluble |
Solubility | H2O: soluble1mg/mL |
Appearance | Powder |
Color | Green-bronze |
Merck | 14,4962 |
BRN | 4115884 |
Storage Condition | Keep in dark place,Inert atmosphere,Room temperature |
Sensitive | Sensitive to light and easy to absorb moisture |
MDL | MFCD00013078 |
Use | Liver function determination diagnostic medication, IR laser dye. |
In vitro study | Cardiogreen-photodynamic therapy (ICG-PDT) at concentrations 1000 μg/mL, induces the significant expression of BAX in HGF cells; however, the laser irradiation as well as ICG shows no significant effects on the expression of BCL-2 gene. |
Hazard Symbols | Xi - Irritant |
Risk Codes | 36/37/38 - Irritating to eyes, respiratory system and skin. |
Safety Description | S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice. S36 - Wear suitable protective clothing. S37/39 - Wear suitable gloves and eye/face protection |
WGK Germany | 3 |
RTECS | DE3150000 |
HS Code | 32129000 |
Toxicity | LD50 intravenous in mouse: 60mg/kg |
Reference Show more | 1. [IF=12.479] Jian Chen et al."A redox-triggered C-centered free radicals nanogenerator for self-enhanced magnetic resonance imaging and chemodynamic therapy."Biomaterials. 2021 Jan;266:120457 2. [IF=6.419] Lili Ren et al."RGD-targeted redox responsive nano micelle: co-loading docetaxel and indocyanine green to treat the tumor."Drug Deliv. 2021;28(1):2024-2032 3. [IF=4.617] Jiang Linxia et al."A dry powder inhalable formulation of salvianolic acids for the treatment of pulmonary fibrosis: safety, lung deposition, and pharmacokinetic study."Drug Deliv Transl Re. 2021 Oct;11(5):1958-1968 4. [IF=3.882] Chenghao Liu et al.Pressure-Controlled Encapsulation of Graphene Quantum Dots into Liposomes by the Reverse-Phase Evaporation Method.Langmuir. 2021;37(48):14096–14104 5. [IF=6.4] Yichi Chen et al."RNA-Seq Explores the Mechanism of Oxygen-Boosted Sonodynamic Therapy Based on All-in-One Nanobubbles to Enhance Ferroptosis for the Treatment of HCC."Int J Nanomed. 2022; 17: 105–123 6. [IF=6.843] Pei-Ying Huang et al."Autophagy-inhibiting biomimetic nanodrugs enhance photothermal therapy and boost antitumor immunity."Biomater Sci-Uk. 2022 Jan;: |
This product is 2-[7-[l, l-dimethyl-3-(4-sulfobutyl)] benzoindolyl)-l,3, 5-heptatrienyl]-1, 1-dimethyl-3-(4-sulfobutyl)-lH-benzo [e] dihydroindole inner salt, sodium salt. The content of C43H47N2NaO6S2 shall be between 94.0% and 105.0% based on the dry product.
take about 0.2g of this product, weigh it accurately, add 0701 ml of water to dissolve it, add 1ml of nitric acid, shake it well, and measure it according to the potentiometric titration method (general rule), with silver nitrate titration solution (0.Olmol/L) titration. Each 1ml of silver nitrate titration solution (0.Olmol/L) is equivalent to 5.0% mg of NaL according to the dry product calculation, containing sodium iodide shall not exceed.
take about 50mg of this product, put it in a 100ml measuring flask, add an appropriate amount of mobile phase, shake to dissolve, dilute to the scale with mobile phase, shake well, and use it as a test solution; take 1 ml accurately, put it in a 100ml measuring flask, dilute it to the scale with mobile phase, shake it well, and use it as a control solution. According to the high performance liquid chromatography (General 0512) test, using eighteen alkyl silane bonded silica gel as filler, phosphate buffer (pH 5.8)-acetonitrile-methanol (50:47:3) as mobile phase, the detection wavelength was 263nm. The number of theoretical plates shall not be less than 1000 calculated by the peak of indocyanine green, and the separation degree between the peak of indocyanine green and the adjacent impurity peaks shall meet the requirements. 10ul of the control solution was injected into the liquid chromatograph, and 10ul of the test solution and the control solution were respectively injected into the liquid chromatograph. The chromatogram was recorded to 2 times of the retention time of the main component peak. If there are impurity peaks in the chromatogram of the test solution, the sum of each impurity peak area shall not be more than 3 times (3.0%) of the main peak area of the control solution.
take this product and dry under reduced pressure at 50°C for 3 hours, and the weight loss shall not exceed 6.0% (General rule 0831).
take this product l. Put it in the crucible, heat it with small fire to carbonize, cool it, add 2ml of nitric acid and 5 drops of sulfuric acid, heat it until the white smoke is removed, and burn it at 500~600°C, make it into ash completely, let it cool down, add 2ml of hydrochloric acid, dry it on a water bath, wet the residue with 3 drops of hydrochloric acid, heat 7ml of water for 2 minutes, add 1 drop of phenolphthalein indicator solution, adjust to slightly red color with ammonia solution, add 0.4ml of dilute acetic acid, transfer to Nessler's colorimetric tube, filter if necessary, wash the Crucible with 2ml of water, and dilute to 10ml of water, as a test solution; another 2ml of nitric acid, 5 drops of sulfuric acid and 2ml of hydrochloric acid were taken, evaporated to dryness, 3 drops of hydrochloric acid were added to wet, 7ml of water was heated for 2 minutes, and 1 drop of phenolphthalein indicator solution was added, adjust to slightly red color with ammonia test solution, add 0.4ml of dilute acetic acid, transfer to another Nessler Cuvette, add 1.0ml of standard lead solution, and dilute to 10ml with water as control solution; add sodium sulfide glycerin solution (take 5g of sodium sulfide, add 10ml of water to dissolve, add 30ml of glycerin to mix, and keep it in the refrigerator for 3 months) 1 drop, shake, place for 5 minutes, check according to law (General 0821), containing heavy metals not more than 10 parts per million.
take 0.25g of this product, add 0.5g of sodium hydroxide, mix, add a small amount of water, stir evenly, after drying, burn with small fire to make charring, then it should be heated at 600~700°C to make it ash completely, and then cooled. Add 5ml of hydrochloric acid and 23ml of water to dissolve the solution, and check according to law (General rule 0822), and it should comply with the regulation (0.0008%).
take an appropriate amount of this product, accurately weigh, add an appropriate amount of methanol to dissolve and quantitatively dilute to prepare a solution containing about 2ug per lml, according to UV-Vis spectrophotometry (General 0401), the absorbance was measured at a wavelength of 784mn and calculated as the absorption coefficient of C43H47N2Na06S2 was 3120.
diagnostic medication.
shade, seal, and store in a cool and dry place.
This product is a sterile freeze-dried product of indocyanine green, containing indocyanine green (C43H47N2Na06S2) should be 90.0% to 110.0% of the label.
This product is a dark green cyan loose solid; Easy to deteriorate when exposed to light and heat.
take 5 pieces of this product, add an appropriate amount of methanol to dissolve indocyanine green respectively, put them in 10ml measuring flask, wash the container with methanol, merge the washing solution into the measuring flask, dilute with methanol to the scale, shake well, take appropriate amount with precision, and dilute quantitatively with methanol to make a solution containing about 2.5ug of indocyanine green per 1 ml, according to UV-Vis spectrophotometry (General rule 0401), the absorbance was measured at a wavelength of 784nm, and the content of each branch was calculated based on the absorption coefficient of C43H47N2Na06S2 being 3120, and the average content of 5 branches was obtained.
Same as indocyanine green.
(l)10mg (2)25mg
light shielding, closed, stored in cold place.