Name | dihydrofluorescein diacetate |
Synonyms | dihydrofluorescein diacetate Dihydroflourescein Diacetate [for Detection of Alcohol and Amines] 2-[3,6-Diacetoxy-9H-xanth-9-yl]benzoic acid 2-(3,6-Diacetoxy-9H-xanthen-9-yl)benzoic acid 2-(3,6-Diacetoxy-9H-xanthen-9-yl)benzenecarboxylic acid Diacetyldihydrofluorescein [for Detection of Alcohol and Amines] Diacetyldihydrofluorescein [for detection of alcohols and amines] |
CAS | 35340-49-9 |
InChI | InChI=1/C24H18O7/c1-13(25)29-15-7-9-19-21(11-15)31-22-12-16(30-14(2)26)8-10-20(22)23(19)17-5-3-4-6-18(17)24(27)28/h3-12,23H,1-2H3,(H,27,28) |
Molecular Formula | C24H18O7 |
Molar Mass | 418.4 |
Density | 1.353±0.06 g/cm3(Predicted) |
Melting Point | 213-215°C(lit.) |
Boling Point | 554.3±50.0 °C(Predicted) |
Flash Point | 190.3°C |
Vapor Presure | 4.01E-13mmHg at 25°C |
Appearance | Powder |
BRN | 364167 |
pKa | 3.85±0.36(Predicted) |
Storage Condition | Room Temprature |
Refractive Index | 1.626 |
MDL | MFCD00005056 |
In vitro study | Dihydrofluorescein diacetate may be a superior fluorescent probe for many cell-based studies. It is a better fluorescent probe for detecting intracellular oxidants because it is more reactive toward specific oxidizing species. Dihydrofluorescein diacetate demonstrates fluorescence of linear structures, consistent with mitochondria, in reoxygenated endothelium. Dihydrofluorescein diacetate is able to detect the presence of ROS in mitochondria. Dihydrofluorescein diacetate fluorescence was sharp and delineated thin filaments which corresponded in all details to TMRM-stained mitochondria. It enters mitochondria and reacts with ROS released in the matrix. Dihydrofluorescein diacetate could be an useful and quantitative method for measuring the oxidative potential of nanoparticle-treated cells. |
Safety Description | S22 - Do not breathe dust. S24/25 - Avoid contact with skin and eyes. |
WGK Germany | 3 |
FLUKA BRAND F CODES | 8-10-23 |
HS Code | 29329990 |
biological activity | Dihydrofluorescein diacetate are fluorescent probes used to measure oxidative stress in cell-free systems and cell models. |