2594-14-1 - Names and Identifiers
Name | trypsin
|
Synonyms | Tryprar Trypsin trypsin Trypsevas Trypsin TPCK α-and β-trypsin Trypsin, Bovine Pancreas Trypsin porcine pancreas trypsin from hog pancreas Trypsin, bovine, USP Grade Trypsin-EDTA Solution 1X trypsin from human pancreas Trypsin TPCK,pancreas basic Trypsin from bovine pancreas Trrpsin,sequence,recombinant Trrpsin,sequence, recombinant Trypsin,recombinant from E.coli Recombinant Trypsin,from E.coli TRYPSIN, PROTEOMICS SEQUENCING GRADE trypsin type ix from porcine pancreas Recombinant Human Trypsin,from E.coli Trypsin, TPCK treated, bovine pancreas Trypsin, DPCC treated, bovine pancreas trypsin tablets 1 mg with buffer salts trypsin type xii-S from bovine pancreas trypsin 1X solution cell culture tested Trypsin, Excision Grade, Bovine Pancreas trypsin 10X solution cell*culture tested trypsin tpck treated from*bovine pancreas Trypsin, Iodination Grade, Human Pancreas trypsin-edta solution cell*culture tested Sequencing recombinant trypsin,from E.coli trypsin-edta solution (1X)*cell culture tested trypsin type xi dpcc treated from*bovine pancreas trypsin-edta solution for endothelial*cell cultur trypsin from porcine pancreas cell*culture tested TRYPSIN-EDTA SOLUTION FOR ENDOTHELIAL*CE LL CULTURES
|
CAS | 9002-07-7 2594-14-1
|
EINECS | 232-650-8 |
2594-14-1 - Physico-chemical Properties
Molecular Formula | C35H47N7O10
|
Molar Mass | 725.78858 |
Density | 1.37[at 20℃] |
Melting Point | 115°C |
Water Solubility | Soluble in water (10 mg/ml), phosphate buffers (10 mg/ml), and balanced salt solutions (1 mg/ml). |
Solubility | Reconstitute in aqueous buffer |
Vapor Presure | 0Pa at 25℃ |
Appearance | lyophilized powder |
Color | White powder |
Odor | Odorless |
Merck | 13,9865 |
pKa | pK1:6.25 (25°C,μ=0.1) |
Storage Condition | -20°C |
Stability | Stable. Incompatible with strong oxidizing agents. |
MDL | MFCD00082094 |
Physical and Chemical Properties | Melting Point: 115°C |
Use | Used for various surgical ulcers and inflammatory gangrene, wound azole injury, fistula hole and other edema, worm |
2594-14-1 - Risk and Safety
Risk Codes | R36/37/38 - Irritating to eyes, respiratory system and skin.
R42 - May cause sensitization by inhalation
R42/43 - May cause sensitization by inhalation and skin contact.
|
Safety Description | S22 - Do not breathe dust.
S24 - Avoid contact with skin.
S26 - In case of contact with eyes, rinse immediately with plenty of water and seek medical advice.
S36/37 - Wear suitable protective clothing and gloves.
S45 - In case of accident or if you feel unwell, seek medical advice immediately (show the label whenever possible.)
S23 - Do not breathe vapour.
|
WGK Germany | 2 |
RTECS | GC3050000 |
FLUKA BRAND F CODES | 1-3-10 |
TSCA | Yes |
HS Code | 35079090 |
2594-14-1 - Nature
Open Data Verified Data
- The proteolytic enzyme extracted from bovine, ovine or porcine pancreas of this strain is white or off-white crystalline powder. Soluble in water, insoluble in ethanol, glycerol, chloroform and ether. It is stable at room temperature on drying. At a pH of 1.8, short boiling is hardly inactivated. The aqueous solution lost 75% of efficacy at room temperature for 3H. The aqueous solution is unstable at pH 6 and quite stable at pH 2~3. The protein precipitates with the addition of salt in the hot solution and the filtrate shows no enzymatic action. Caz + has protective and activating effects. Isoelectric point pl = 10.1.
- bovine trypsinogen consists of 229 amino acid residues, containing 6 pairs of disulfide bonds. The peptide bond between the N-terminal lysine and the isoleucine residue of the zymogen is hydrolyzed under the catalysis of enterokinase or autocatalysis, and the peptide "day" day "day" day "Lai 6 is released, became active trypsin, molecular weight 24000, is a single peptide chain consisting of 223 amino acid residues. Porcine trypsin is more stable than trypsin, and the unit is higher than that of cattle. Its isoelectric point pl = 10.8.
- trypsin acts exclusively on a peptide bond composed of the basic amino acids arginine and leucine carboxyl. The enzyme itself is very easy to dissolve, by the original B trypsin into a-trypsin, and then further degradation of trypsin and even fragments, the vitality is gradually decreased and lost. Since serum contains non-specific trypsin inhibitors, trypsin does not digest normal tissues. It can improve tissue permeability, strongly inhibit experimental edema, inhibit inflammation around the thrombus; Can quickly dissolve blood clots, exudate and necrotic tissue; Intravenous injection can prolong clotting time, but large doses instead promote blood coagulation, and even cause heart disorders; Decomposition of sputum, pus and other viscous secretions; Can promote the penetration of antibiotics, chemotherapy and other drugs to the lesion; A significant solution of snake venom.
Last Update:2024-01-02 23:10:35
2594-14-1 - Preparation Method
Open Data Verified Data
- extraction method using bovine pancreas as raw material.
- extraction method using sheep pancreas as raw material.
- extraction method using porcine pancreas as raw material.
- after the pig pancreas was washed and cleaned, it was minced, and an aqueous acetic acid solution having a pH of 4 was added thereto. The mixture was stirred and extracted at 4 ° C. Or lower for 24 hours, followed by filtration. Ammonium sulfate was added to the filtrate to bring the concentration to 75% of saturation, and the mixture was centrifuged to obtain a salting-out precipitate. The supernatant was then salted out and precipitated [(NH4)2 S04 85% saturation], and separated by adsorption, and ribonuclease A was produced. 90g of the salting out precipitate was dissolved in 1L of distilled water, then 30g of CaCl2 powder was added, and the pH was adjusted to 8, then 5mg of crystalline trypsin was added, activated at 4 °c for 24h, filtered, filter off the precipitate and adjust the pH to 7.8, add p-aminobenzamidine-Sepharose 6B resin for Batch adsorption, then use 2 times the resin volume of pH 7.8 buffer (containing CaCl2,0.1 mol/L) washing, then loading column, and then formic acid (0.1 mol/L) -KCl(0.05 mol/L), pH 2.2 buffer solution elution, activity peak collection, dialysis, freeze drying, available trypsin.
Last Update:2022-01-01 11:10:49
2594-14-1 - Standard
Authoritative Data Verified Data
proteolytic enzymes extracted from pig, sheep or bovine pancreas in this line. Calculated on a dry basis, trypsin activity per 1 mg should not be less than 2500 units.
Last Update:2024-01-02 23:10:35
2594-14-1 - Trait
Authoritative Data Verified Data
This product is white or off-white crystalline powder.
Last Update:2022-01-01 15:38:07
2594-14-1 - Use
Open Data Verified Data
- proteolytic enzyme. Surgery for a variety of inflammation, ulcers, gangrene, trauma, fistula hole caused by local abscess, edema, hematoma, but also for snake bites and other diseases. Dermatology is used to treat ringworm and other skin disorders. Internal medicine for empyema, emphysema, bronchitis, bronchial asthma and other diseases. It is also used in chemical industry, light industry, textile and other industries.
- usage and dosage before use, add appropriate amount of sodium chloride injection to dissolve. Intramuscular injection: Local injection or spray inhalation, the amount of the disease depending on the decision.
Last Update:2022-01-01 11:10:50
2594-14-1 - Differential diagnosis
Authoritative Data Verified Data
take about 2mg of this product, put it on a white drip plate, add 0.2 of p-Toluenesulfonyl-L-arginine methyl ester hydrochloride test solution, stir well, then appear purple.
Last Update:2022-01-01 15:38:10
2594-14-1 - Safety
Open Data Verified Data
- pain at the site of intramuscular injection, with induration, chills, Fever, Head Pain, chest pain, Abdominal Pain, Dyspnea, rash, angioedema, elevated intraocular pressure, leukopenia and other adverse reactions, but does not affect the drug; Generally given antihistamines and antidotes can be controlled; Rare allergic shock; Tuberculous empyema, tracheal pleural fistula in patients with caution. Can not be used for acute inflammation, bleeding cavity, pulmonary hemorrhage within one week; Liver, kidney injury, blood coagulation dysfunction and bleeding in patients with no use.
- light-shielding, closed, stored in a cool and dry place.
Last Update:2022-01-01 11:10:50
2594-14-1 - Exam
Authoritative Data Verified Data
acidity
take this product, add water to dissolve and dilute the solution containing 2mg per lml, according to the law (General 0631),pH value should be 5.0~7.0.
clarity of the solution
take this product, add 0.9% sodium chloride solution to dissolve and dilute the solution containing lOmg per lml, check according to law (General Principles 0902 first method), the solution should be clarified.
chymotrypsin
preparation of substrate solution N-acetyl-L-tyrosine ethyl ester 23.7mg was placed in a volumetric flask, add phosphate buffer (0.067mol/L potassium dihydrogen phosphate solution 38.9ml and 0.067mol/L disodium hydrogen phosphate solution 61.1ml, mixed, pH 7.0)50ml, warm to dissolve, after cooling, dilute to the scale and shake. Frozen storage, but not repeated freeze-thaw. Preparation of Test Solution take appropriate amount of this product, precision weighing, add 0.OOlmol/L hydrochloric acid solution to dissolve and quantitatively dilute to prepare a solution containing 0.25mg per 1 ml.
2.0ml of the substrate solution and 7.0 M L of a 0. O01 mol/l hydrochloric acid solution were mixed with 1 ml of the above phosphate buffer (pH) to prepare a blank. Take 0.2ml of the test solution accurately, add 0.5 ml of the substrate solution (preheated to 25°C ± 3.0°C), immediately Time and shake, keep the temperature in the cuvette at 25°C ± 0.5°C and read the absorbance at a wavelength of 237nm every 30 seconds for 5 minutes by UV-Vis spectrophotometry (General 0401 ) , the rate of change of absorbance should be constant every 30 seconds, and the constant time should not be less than 3 minutes.
loss on drying
take an appropriate amount of this product, take phosphorus pentoxide as desiccant, and dry under reduced pressure at 60°C for 4 hours. The weight loss shall not exceed 5.0% (General rule 0831).
Last Update:2022-01-01 15:38:12
2594-14-1 - Titer determination
Authoritative Data Verified Data
preparation of substrate solution
take N-benzoyl-L-Arginine ethyl ester hydrochloride 85.7mg, add water to make 100ml, as a substrate stock solution; Take 10ml, dilute with phosphate buffer (0.067mol/L potassium dihydrogen phosphate solution 13ml mixed with 0.067mol/L disodium hydrogen phosphate solution 87ML, pH 7.6) to ML, according to UV-visible spectrophotometry (General rule 0401), constant temperature at 25.0°C ± 0.5°C, with water as blank, absorbance was measured at the wavelength of 253nm, if necessary, the substrate solution can be used by adjusting the above substrate stock solution or phosphate buffer so that the absorbance is between 0.575 and 0.585. Should be used within 2 hours after preparation.
preparation of test solution
precision weigh the appropriate amount of this product, add 0.001mol/L hydrochloric acid solution to dissolve and quantitatively dilute to make a solution containing 50 to 60 trypsin units per 1 ml.
assay
3.0 of the substrate solution was collected, and 200 u1 of a 0. O01 mol/L hydrochloric acid solution was added thereto, and the mixture was mixed uniformly to prepare a blank. Another precision sample 200ul solution, plus substrate solution (constant temperature at 25.0°C ± 0.5°C)3.0, immediately timing, mixing, the temperature in the cuvette was maintained at 25.0 ° C. ± 0.5 ° C., and the absorbance was read at a wavelength of 0401 NM every 30 seconds for 5 minutes by UV-visible spectrophotometry (general). The absorbance is plotted on the ordinate and the time is plotted on the abscissa; The change of absorbance should be constant between 0.015 and 0.018 every 30 seconds, and the time in a linear relationship should not be less than 3 minutes.
Last Update:2022-01-01 15:38:14
2594-14-1 - Category
Authoritative Data Verified Data
Last Update:2022-01-01 15:38:14
2594-14-1 - Storage
Authoritative Data Verified Data
shade, seal, and store in a cool and dry place.
Last Update:2022-01-01 15:38:14
2594-14-1 - Legal requirements
Authoritative Data Verified Data
This product should be extracted from Quarantine qualified cattle, sheep or pig pancreas, the species of animals used should be clear, the production process should comply with the current version of the "good manufacturing practice" requirements.
Last Update:2022-01-01 15:38:15
2594-14-1 - Trypsin for injection
Authoritative Data Verified Data
This product is a sterile lyophilized product of trypsin. Trypsin-containing viability units should be between 90.0% and 120.0% of the labeled amount.
trait
This product is white or off-white lyophilized cake or powder.
identification
take about 5000 units of this product and show the same reaction according to the identification test under trypsin.
examination
- acidity take this product, add water to dissolve and dilute to make a solution containing 5000 units per lml, and determine it according to law (General 0631). The pH value should be 5.0~7.0.
- the color of the solution is taken from this product, and 0.9% sodium chloride solution is added to dissolve and dilute to make a solution containing 25,000 units per 1 ml, which should be colorless; If color development occurs, comparison with yellow No. 2 Standard Colorimetric solution (General rule 0901 first method), not deeper.
- loss on drying: take about 0.2g of this product, use phosphorus pentoxide as desiccant, and dry under reduced pressure at 60°C for 4 hours. The loss of weight shall not exceed 8.0% (General rule 0831).
- abnormal toxicity take this product, add Sterile Water for Injection dissolved and diluted into 125 units per lml of solution, according to law inspection (General 1141), should comply with the provisions.
- sterile take this product, after dissolving with appropriate solvent, the membrane filtration method, according to the law inspection (General 1101), should comply with the provisions.
- others should comply with the relevant provisions under injection (General 0102).
titer determination
take 5 pieces of this product, add appropriate amount of 0.001mol/L hydrochloric acid solution to dissolve, and transfer the whole amount to the same 100ml measuring flask, dilute to the scale with the above hydrochloric acid solution, shake, an appropriate amount was accurately measured and quantitatively diluted with the above hydrochloric acid solution to prepare a solution containing about 50 to 60 units per 1 ml. The assay was performed according to the method under trypsin.
category
Same as trypsin.
specification
(1) 12,500 units (2) 25,000 units (3) 50,000 units (4) 100,000 units
storage
sealed and kept in a cool dark place.
Last Update:2022-01-01 15:38:15
2594-14-1 - Trait
White or off-white powder.
Last Update:2023-08-16 21:32:38
2594-14-1 - Source
Bovine pancreas.
Last Update:2023-08-16 21:32:38
2594-14-1 - Loss on drying
≤5.0%。
Last Update:2023-08-16 21:32:38