Nature:
2-C-Methyl-1,3, is a white crystalline solid with good solubility and can be dissolved in water and most organic solvents. Its molecular formula is C21H22N2O6 and its molecular weight is 398.41g/mol.
Use:
2-C-Methyl-1,3, is mainly used as a chromogenic substrate for enzymes in biochemical experiments. It can be enzymatically reduced to a purple product, a property that makes it very useful in studying cell proliferation and cytotoxicity. In addition, MTT can also be used for the determination of cell viability and cell proliferation.
Method:
The preparation method of 2-C-Methyl-1,3, is relatively complicated. The general synthetic route involves multiple reaction steps including the synthesis and acylation of the furan ring. Due to the chemical reactions involved, the synthesis process needs to be carried out under appropriate experimental conditions to ensure safety and high purity of the product.
Safety Information:
2-C-Methyl-1,3, is generally relatively safe under normal experimental conditions, but the following matters still need to be paid attention:
-It is an organic compound with certain toxicity. Direct contact with skin and eyes should be avoided.
-In the use of the process should follow the laboratory safety procedures, the use of appropriate protective measures, such as wearing gloves, protective glasses and laboratory exhaust equipment.
-Observe relevant chemical storage and waste handling regulations when storing and handling.