Molecular Formula | C20H15N6NaO11S2 |
Molar Mass | 602.48 |
Storage Condition | under inert gas (nitrogen or Argon) at 2-8°C |
Use | Screening of Cell Proliferation Detects |
In vitro study | The generally used MTS-based assay is compared with a bioassay employing a water-soluble tetrazolium dye, WST-8, using NFS-60 cells at a concentration of 7×10 5 cells/mL against 800 IU/mL of PEGylated G-CSF at 24, 48, 72, and 72 h time points to determine the efficacy of PEGylated G-CSF. Further, the optimized WST-8 dye-based assay is used to test the potency of various commercially available PEGylated G-CSF preparations. The results demonstrate enhanced sensitivity of the WST-8-based assay over the conventional MTS-based assay for determining the potency of PEGylated G-CSF using the NFS-60 cell line. |
Reference Show more | 1. Zhang, Zhe, et al. "Lactobacillus casei YRL577 combined with plant extracts reduce markers of non-alcoholic fatty liver disease in mice." British Journal of Nutrition 125.10 (2021): 1081-1091https://doi.org/10.1017/S0007114520003013 2. Li, Guangzhang, et al. "miR‐505 inhibits proliferation of osteosarcoma via HMGB1." FEBS open bio 10.7 (2020): 1251-1260.https://doi.org/10.1002/2211-5463.12868 |