Molecular Formula | C29H37NO5 |
Molar Mass | 479.61 |
Density | 1.1490 (rough estimate) |
Melting Point | 218-223 °C |
Boling Point | 577.96°C (rough estimate) |
Flash Point | 85℃ |
Solubility | ethanol: 20mg/mL |
Vapor Presure | 4.98E-23mmHg at 25°C |
Appearance | powder |
Color | white |
Merck | 13,2819 |
BRN | 1096207 |
pKa | 13.60±0.70(Predicted) |
Storage Condition | -20°C |
Stability | Stable for 2 years from date of purchase as supplied. Solutions in DMSO may be stored at -20°C for up to 1 month. |
Refractive Index | 1.6290 (estimate) |
In vitro study | Cytochalasin B is a cell-permeable mycotoxin binding to the barbed end of actin filaments, inhibits the enlongation and shortening of actin filaments, with K d s of 2.2 nM and 1.4 nM for F-actin in the presence of MgCl 2 (2 mM) or MgCl 2 (2 mM) plus KCl, respectively. Cytochalasin B (0.1-10 μM) shows inhibitory effect on multiple murine cancer cell lines, with IC 50 s of 2.56 μM (M109c), 10.46 μM (B16BL6), 105.5 μM (P388/ADR), 51.9 μM (P388/S) and IC 80 s of 12.23 μM (M109c), 44.86 μM (B16BL6), 188.4 μM (P388/ADR), 84.1 μM (P388/S) after treatment for 3 h, with IC 50 s of 0.25 μM (M109c), 0.37 μM (B16F10), 0.87 μM (B16BL6), and IC 80 s of 0.75 μM (M109c), 1.21 μM (B16F10), 10.41 μM (B16BL6) after treatment for 4 days. Cytochalasin B (6 μM) increases the myofibrillar fragmentation index (MFI), which is attributed to the intensely breaking of myofibrillar proteins into short segments. Cytochalasin B also accelerates the disruption of actin filaments. In addition, Cytochalasin B accelerates the transformation from F-actin to G-actin, lowering the content of F-actin and significantly increasing G-actin bands during postmortem conditioning. |
In vivo study | Cytochalasin B (10, 25, 50 mg/kg, i.p.) dose-dependently increases the life expectancy of Balb/c mice bearing with P388/ADR leukemias. Cytochalasin B at 50 mg/kg produces 10 % long-term survival in the multidrug resistant P388/ADR cohort, and 40 % long-term survival in the drug sensitive P388/S cohort. |
Risk Codes | R26/27/28 - Very toxic by inhalation, in contact with skin and if swallowed. R63 - Possible risk of harm to the unborn child R23/24/25 - Toxic by inhalation, in contact with skin and if swallowed. |
Safety Description | S28 - After contact with skin, wash immediately with plenty of soap-suds. S36/37 - Wear suitable protective clothing and gloves. S45 - In case of accident or if you feel unwell, seek medical advice immediately (show the label whenever possible.) |
UN IDs | UN 1544 6.1/PG 2 |
WGK Germany | 3 |
RTECS | RO0205000 |
FLUKA BRAND F CODES | 10 |
HS Code | 29349990 |
Hazard Class | 6.1(a) |
Packing Group | I |
Toxicity | LD50 intraperitoneal in mouse: 30mg/kg |