|Molar Mass||504.44 g/mol|
|Melting Point||198-200 °C|
|Boling Point||902.9±65.0 °C(Predicted)|
|Physical and Chemical Properties||Chemical properties of white crystalline powder, soluble in methanol, ethanol, DMSO and other organic solvents, derived from sugarcane, Morinda officinalis.|
|Safety Description||24/25 - Avoid contact with skin and eyes.|
objective to establish a dual wavelength thin layer chromatography scanning method for the determination of the content of GF2 in the different time and the origin of the sugar. Methods with GF2 as the standard, using dual wavelength thin layer scanning analysis, the developing solvent was glacial acetic acid-chloroform-95% ethanol (9:11:11), the color reagent is diphenylamine-aniline-acetone-phosphoric acid (1:1:50:5), the maximum absorption wavelength is 510nm, the reference wavelength is 610nm. The result showed that the linear range of GF2 was 0.642 8~15.975 2 μg (r = 0.999 2). The average recovery was 102.5% (RSD = 1.55%). The content of GF2 was 12.957 3~36.328 1 mg/g in different producing areas. Conclusion The dual wavelength TLC scanning method has good separation effect and simple operation, and can be used for the qualitative and quantitative analysis of GF2 in spinose.
objective: to establish a method for the determination of fructose triose (GF2) in pricking sugar by high performance liquid chromatography with refractive index detection (HPLC-RID), the content of GF2 in 10 different batches of thorn sugar was determined. Methods a YMC-Pack NH2 column (250 × 4.6mm) was used. D., s-5μm, 12 nm); Mobile phase: acetonitrile-water = 75:25, flow rate: 1.0/min, injection volume: 30 μL. Results the linear range of GF2 was 0.808~1.616 mg/mL, r = 0.9994; The relative standard deviation of precision was 0.57%; The recovery rate of low, medium and high concentration was 97.8%,96.1%, respectively, 104.3%, the relative standard deviation were 1.8%,1.8%,1.6%. The highest content of GF2 in 10 batches was 454.57 mg/g, and the lowest was 115.90 mg/g. Conclusion The method has good precision and accuracy, and can be used for the qualitative and quantitative analysis of GF2 in Thorn sugar.
fructosyl transferase from Aspergillus oryzae was used to catalyze the process of sucrose reaction to produce sucrose triose in ionic liquid. The effects of salt balance, ratio of substrate sucrose and enzyme amount, temperature, time, pH, ratio of ionic liquid and enzyme amount on the catalytic yield were investigated, the water activity of the ionic liquid is controlled by the hydrated salt composed of high hydrate and low hydrate of the salt. The results showed that the optimal reaction conditions were as follows: reaction temperature 40 ℃,pH 6.2, substrate concentration 60%, reaction time 24h. With regard to the reuse of ionic liquids and enzymes, the properties of ionic liquids and the catalytic activity of enzymes need to be further studied.