取猪血2kg,加入柠檬酸钠溶液200mL(内含柠檬酸钠38g),混匀,离心分离。取上清液血浆,加入蒸馏水稀释,用300mL的1%乙酸调节pH为5.3,待沉淀完全,收集沉淀,得凝血酶原。取凝血酶原,在25~30℃下,加入氯化钠溶液使其溶解,再在每升溶液中加入15g氯化钙,充分搅拌后,冷室静置th,离心分离。上清液中加冷冻丙酮,搅拌均匀后,静置过夜。过滤,沉淀用乙醚洗涤,干燥,得凝血酶粗品。粗品中加入氯化钠溶液使其溶解,在O℃放置6h,过滤。滤液再用乙酸调节pH为5.5,离心分离,然后取上清液加入2倍量冷冻丙酮,静置2h,待沉淀完全后,离心分离。沉淀再分别用无水乙醇、乙醚洗涤,真空干燥,可制得凝血酶。
中文名 | 凝血酶 |
英文名 | Thrombin |
别名 | 凝血酶 凝血酵素 凝血醇素 凝血酶,牛血浆 凝血酶,凝血因子 凝血酶(牛血浆) 凝血酶,老鼠中提取 凝血酶,人体中提取 |
英文别名 | C00752 Thrombin THROMBIN thrombin time THROMBIN, BOVINE Thrombin, Bovine Thrombin, Human Plasma thrombin from human plasma thrombin from bovine plasma Blood coagulation factor IIa Thrombin, Plasminogen-Free, Bovine Thrombin, Citrate-Free, HumanPlasma Thrombin from human plasma,Factor IIa Thrombin from murine plasma,Factor IIa Thrombin from bovine plasma,Factor IIa |
CAS | 9002-04-4 |
EINECS | 232-648-7 |
熔点 | 47.7 °C |
溶解度 | H2O: 1毫升/小瓶澄清至轻微混浊,无色溶液 |
存储条件 | -20°C |
外观 | 粉末 |
颜色 | white |
Merck | 13,9461 |
物化性质 | 凝血酶为牛血或猪血中提取的凝血酶原,经激活而得凝血酶的无菌冻干品,为白色或类白色的冻干块状物或粉末。干粉在2~80C储存很稳定,干粉易溶于水,0.9%的氯化钠溶液显浑浊,不溶于有机溶剂。在室温水溶液8h内失活。故须临用时新鲜配制,遇热、称酸、碱、金属等活力降低。 |
MDL号 | MFCD00166053 |
危险品标志 | Xn - 有害物品 Xi - 刺激性物品 |
风险术语 | R36/37/38 - 刺激眼睛、呼吸系统和皮肤。 R42 - 吸入可能致敏。 |
安全术语 | S22 - 切勿吸入粉尘。 S24/25 - 避免与皮肤和眼睛接触。 S36/37 - 穿戴适当的防护服和手套。 S26 - 不慎与眼睛接触后,请立即用大量清水冲洗并征求医生意见。 S24 - 避免皮肤接触。 |
WGK Germany | 2 |
RTECS | XO8950000 |
TSCA | Yes |
海关编号 | 30021099 |
上游原料 | 二水合柠檬酸钠 |
参考资料 展开查看 | 1. 白景芝 刘永杰. 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取猪血2kg,加入柠檬酸钠溶液200mL(内含柠檬酸钠38g),混匀,离心分离。取上清液血浆,加入蒸馏水稀释,用300mL的1%乙酸调节pH为5.3,待沉淀完全,收集沉淀,得凝血酶原。取凝血酶原,在25~30℃下,加入氯化钠溶液使其溶解,再在每升溶液中加入15g氯化钙,充分搅拌后,冷室静置th,离心分离。上清液中加冷冻丙酮,搅拌均匀后,静置过夜。过滤,沉淀用乙醚洗涤,干燥,得凝血酶粗品。粗品中加入氯化钠溶液使其溶解,在O℃放置6h,过滤。滤液再用乙酸调节pH为5.5,离心分离,然后取上清液加入2倍量冷冻丙酮,静置2h,待沉淀完全后,离心分离。沉淀再分别用无水乙醇、乙醚洗涤,真空干燥,可制得凝血酶。
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