中文名 | 碘化丙啶 |
英文名 | propidium iodide |
别名 | 碘丙啶 碘化丙碇 碘化丙啶 PI染色液 碘化丙碇(PI) 碘化丙啶PI *10MM水溶液* 碘化丙啶 PROPIDIUM IODIDE |
英文别名 | propidium iodide Propidium iodide solution 3,8-DIAMINO-5-[3-(DIETHYLMETHYLAMMONIO)PROPYL]-6-PHENYLPHENANTHRIDINIUM 3,8-diamino-5-(3-(diethylmethylammonio)propyl)-6-phenyl-phenanthridiniudii 3,8-DIAMINO-5-(3-[DIETHYLMETHYLAMMONIO])-6-PHENYLPHENAN-THRIDINIUM DIIODIDE 3,8-diamino-5-(3-diethylaminopropyl)-6-phenylphenanthridiniumiodidemethiodi 3,8-diamino-5-(3-(diethylmethylammonio)propyl)-6-phenylphenanthridiniumdiiod 3,8-DIAMINO-5-(3-DIETHYL-AMINOPROPYL)-6-PHENYLPHENANTHRIDIUM IODIDE METHIODIDE 3,8-DIAMINO-5-(3-DIETHYLAMINOPROPYL)-6-PHENYLPHENANTHRIDINIUM IODIDE METHIODIDE 3,8-DIAMINO-5-[3-(DIETHYLMETHYLAMMONIO)PROPYL]-6-PHENYLPHENANTHRIDINIUM DIIODIDE 3,8-diamino-5-{3-[diethyl(methyl)ammonio]propyl}-6-phenylphenanthridinium diiodide 3-(3,8-diamino-6-phenylphenanthridin-5(6H)-yl)-N,N-diethyl-N-methylpropan-1-aminium |
CAS | 25535-16-4 |
EINECS | 247-081-0 |
化学式 | C27H34IN4+ |
分子量 | 541.5 |
InChI | InChI=1/C27H35N4/c1-4-31(3,5-2)17-9-16-30-26-19-22(29)13-15-24(26)23-14-12-21(28)18-25(23)27(30)20-10-7-6-8-11-20/h6-8,10-15,18-19,27H,4-5,9,16-17,28-29H2,1-3H3/q+1 |
密度 | 1.5072 (estimate) |
熔点 | 220-225 °C (dec.) (lit.) |
水溶性 | Soluble in water at 1mg/ml |
溶解度 | 很难混合。 |
存储条件 | 2-8°C |
敏感性 | Light Sensitive & Hygroscopic |
外观 | 片状粉末 |
颜色 | Dark red |
最大波长(λmax) | ['493 nm, 535 nm'] |
BRN | 3843838 |
MDL号 | MFCD00011921 |
体外研究 | Propidium Iodide is a cell-membrane impermeable dye with characteristic excitation maximum at 535 nm and emission maximum at 617 nm which intercalates with nucleic acids with a stoichiometry of one dye per 4-5 base pairs with little sequence preference. Propidium Iodide has evidenced of having no toxic effects on neurons, being today’s most common marker for membrane integrity and cell viability when applied prior to fixation (pre-fixation Propidium Iodide staining method). The pre-fixation staining has been widely used for quantitative assessments of neuronal cell decline in models of acute neurodegeneration, visualized as intensely labeled PI + -pycnotic nuclei of degenerating neurons . Propidium Iodide cannot cross the membrane of live cells, making it useful to measure the percentage of apoptotic cells by flow-cytometric analysis. The flow cytometric data shows an excellent correlation with the results obtained with both electrophoretic and colorimetric methods. This new rapid, simple and reproducible method proves useful for assessing apoptosis of specific cell populations in heterogeneous tissues such as bone marrow, thymus and lymph nodes. |
危险品标志 | Xi - 刺激性物品 Xn - 有害物品 |
风险术语 | R36/37/38 - 刺激眼睛、呼吸系统和皮肤。 R68 - 可能有不可逆后果的危险。 R67 - 蒸汽可能引起困倦和眩晕。 R48/20/22 - R40 - 少数报道有致癌后果。 R36/38 - 刺激眼睛和皮肤。 R22 - 吞食有害。 R20 - 吸入有害。 R63 - 可能有对胎儿造成伤害的危险。 |
安全术语 | S26 - 不慎与眼睛接触后,请立即用大量清水冲洗并征求医生意见。 S36 - 穿戴适当的防护服。 S22 - 切勿吸入粉尘。 S36/37 - 穿戴适当的防护服和手套。 |
WGK Germany | 3 |
RTECS | SF7949600 |
FLUKA BRAND F CODES | 3-8-10-23 |
海关编号 | 29339900 |
参考资料 展开查看 | 1. 翁亚菡 鲍伟杰 梁瑞敏 等. MAPK信号通路调控子宫内膜癌细胞增殖、侵袭和迁移的影响[J]. 临床与病理杂志 2019 000(003):P.470-478. 2. 史文艳 孙震. ε-聚赖氨酸盐酸盐与Nisin对蜡状芽孢杆菌的协同作用及机理[J]. 食品与机械 2019 v.35;No.209(03):21-25+109. 3. 张珂, 邓清华, 马胜林. 重楼醇提取物对胃癌SGC-7901细胞增殖和凋亡的影响[J]. 中华中医药学刊, 2016. 4. 曼,蔡林衡,杨海平,杨学文,司晓慧.DR4去甲基化对髓性白血病K562细胞增殖凋亡作用的研究[J].中国实验血液学杂志,2021,29(02):422-427. 5. Yang, Huirong, et al. "Improvement of multiple-stress tolerance and ethanol production in yeast during very-high-gravity fermentation by supplementation of wheat-gluten hydrolysates and their ultrafiltration fractions." Journal of agricultural and food che 6. [IF=6.953] Yu-Heng Mao et al."Protection of Bifidobacterial cells against antibiotics by a high molecular weight exopolysaccharide of a medicinal fungus Cs-HK1 through physical interactions."Int J Biol Macromol. 2018 Nov;119:312 7. [IF=5.279] Huirong Yang et al."Improvement of Multiple-Stress Tolerance and Ethanol Production in Yeast during Very-High-Gravity Fermentation by Supplementation of Wheat-Gluten Hydrolysates and Their Ultrafiltration Fractions."J Agr Food Chem. 2018;66(39):10233–1024 8. [IF=4.952] Huirong Yang et al."Efficient fermentation of very high-gravity worts by brewer's yeast with wheat gluten hydrolysates and their ultrafiltration fractions supplementations."Lwt Food Sci Technol. 2019 Jun;106:151 9. [IF=3.31] Xiaoxiao Wang et al."2D Dendritic Gold Nanostructures Formed on Silica Nanosheets: Transferability, Clean Surface, and Their Biomedical Application."Part Part Syst Char. 2018 Oct;35(10):1800268 10. [IF=15.419] Wei Wang et al."Gold-Nanoshell-Functionalized Polymer Nanoswimmer for Photomechanical Poration of Single-Cell Membrane."J Am Chem Soc. 2019;141(16):6601–6608 11. [IF=7.79] Simeng Liu et al."Adoptive CD8+T-cell grafted with liposomal immunotherapy drugs to counteract the immune suppressive tumor microenvironment and enhance therapy for melanoma."Nanoscale. 2021 Oct;13(37):15789-15803 12. [IF=7.446] Jun Liu et al."Novel 2-Substituted 3-Hydroxy-1,6-dimethylpyridin-4(1H)-ones as Dual-Acting Biofilm Inhibitors of Pseudomonas aeruginosa."J Med Chem. 2020;63(19):10921–10945 13. [IF=7.086] Yinghan Liu et al."Inactivation of antibiotic resistant bacterium Escherichia coli by electrochemical disinfection on molybdenum carbide electrode."Chemosphere. 2022 Jan;287:132398 14. [IF=6.953] Zhou Dong et al."Purification and comparative study of bioactivities of a natural selenized polysaccharide from Ganoderma Lucidum mycelia."Int J Biol Macromol. 2021 Nov;190:101 15. [IF=4.375] Zou Lei et al."miR-651-3p Enhances the Sensitivity of Hepatocellular Carcinoma to Cisplatin via Targeting ATG3-Mediated Cell Autophagy."J Oncol. 2021;2021:5391977 16. [IF=3.463] Yuan-Shan Zhang et al."Induction of tetraploids in ‘Red Flash’ caladium using colchicine and oryzalin: Morphological, cytological, photosynthetic and chilling tolerance analysis."Sci Hortic-Amsterdam. 2020 Oct;272:109524 17. [IF=11.277] Sun Guiling et al."The maize single-nucleus transcriptome comprehensively describes signaling networks governing movement and development of grass stomata."Plant Cell. 2022 Feb;: |